Summary of Study ST000195

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000166. The data can be accessed directly via it's Project DOI: 10.21228/M8S01D This work is supported by NIH grant, U2C- DK119886.

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Study IDST000195
Study TitleACSL5 Whole Body Bile Acids
Study SummaryUnderstand the differences in Bile Acid composition between knockout and floxed mice at each intestinal segment. Also difference in metabolites between these two groups at each level of the intestine.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-09
Num Groups7
Total Subjects52
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size128 M
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8S01D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000166
Project DOI:doi: 10.21228/M8S01D
Project Title:ASCL5 Bile Acids Analysis
Project Summary:Analysis of Bile Acids in Intestinal Segments of Chow Fed Male ACSL5 Knockout Mice
Institute:Tufts University
Department:Endocrinology, Diabetes, and Metabolism
Laboratory:Greenberg Lab
Last Name:Greenberg
First Name:Andrew
Address:Medford, MA
Email:andrew.greenberg@tufts.edu
Phone:617-556-3144

Subject:

Subject ID:SU000214
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Intestinal Segment
SA009785S00015200FLOX Duodenum
SA009786S00015236FLOX Duodenum
SA009787S00015233FLOX Duodenum
SA009788S00015218FLOX Duodenum
SA009789S00015224FLOX Duodenum
SA009790S00015215FLOX Duodenum
SA009791S00015209FLOX Duodenum
SA009792S00015239FLOX Duodenum
SA009793S00015220FLOX Illeum
SA009794S00015226FLOX Illeum
SA009795S00015202FLOX Illeum
SA009796S00015241FLOX Illeum
SA009797S00015238FLOX Illeum
SA009798S00015217FLOX Illeum
SA009799S00015235FLOX Illeum
SA009800S00015211FLOX Illeum
SA009801S00015216FLOX Jejunum
SA009802S00015234FLOX Jejunum
SA009803S00015240FLOX Jejunum
SA009804S00015237FLOX Jejunum
SA009805S00015225FLOX Jejunum
SA009806S00015219FLOX Jejunum
SA009807S00015210FLOX Jejunum
SA009808S00015201FLOX Jejunum
SA009809S00015227Knockout Duodenum
SA009810S00015203Knockout Duodenum
SA009811S00015242Knockout Duodenum
SA009812S00015206Knockout Duodenum
SA009813S00015197Knockout Duodenum
SA009814S00015230Knockout Duodenum
SA009815S00015194Knockout Duodenum
SA009816S00015212Knockout Duodenum
SA009817S00015221Knockout Duodenum
SA009818S00015232Knockout Illeum
SA009819S00015208Knockout Illeum
SA009820S00015205Knockout Illeum
SA009821S00015229Knockout Illeum
SA009822S00015244Knockout Illeum
SA009823S00015223Knockout Illeum
SA009824S00015196Knockout Illeum
SA009825S00015199Knockout Illeum
SA009826S00015214Knockout Illeum
SA009827S00015195Knockout Jejunum
SA009828S00015243Knockout Jejunum
SA009829S00015213Knockout Jejunum
SA009830S00015204Knockout Jejunum
SA009831S00015207Knockout Jejunum
SA009832S00015222Knockout Jejunum
SA009833S00015228Knockout Jejunum
SA009834S00015231Knockout Jejunum
SA009835S00015198Knockout Jejunum
Showing results 1 to 51 of 51

Collection:

Collection ID:CO000202
Collection Summary:-
Sample Type:Flushing

Treatment:

Treatment ID:TR000222

Sample Preparation:

Sampleprep ID:SP000216
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000297
Analysis type MS
Chromatography type Reversed phase
Chromatography system
Column Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units µM

Chromatography:

Chromatography ID:CH000218
Methods ID:AQM041
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-h.m.zip
Column Name:Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000246
Analysis ID:AN000297
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:2014-07-30-Bile_acid-full-BEH-C18-h.m.zip
Processing Parameters File:EX00322-Bile-Acids-Quant-method.m.zip
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