Summary of Study ST000201
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000156. The data can be accessed directly via it's Project DOI: 10.21228/M8XS3B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000201 |
| Study Title | Bone Marrow Metabolomics (part II) |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | Women of reproductive age are protected from metabolic disease relative to postmenopausal women and men. Most preclinical rodent studies are skewed toward the use of male mice to study obesity-induced metabolic dysfunction because of a similar protection observed in female mice. How sex differences in obesity-induced inflammatory responses contribute to these observations is unknown. We have compared and contrasted the effects of high fat diet-induced obesity on glucose metabolism and leukocyte activation in multiple depots in male and female C57Bl/6 mice. With both short term and long term high fat diet, male mice demonstrated increased weight gain and CD11c(+) adipose tissue macrophage content compared with female mice despite similar degrees of adipocyte hypertrophy. Competitive bone marrow transplant studies demonstrated that obesity induced a preferential contribution of male hematopoietic cells to circulating leukocytes and adipose tissue macrophages compared with female cells independent of the sex of the recipient. Sex differences in macrophage and hematopoietic cell in vitro activation in response to obesogenic cues were observed to explain these results. In summary, this report demonstrates that male and female leukocytes and hematopoietic stem cells have cell-autonomous differences in their response to obesity that contribute to an amplified response in males compared with females. The research is published, but data obtained by core not included in the paper: http://www.jbc.org/cgi/pmidlookup?view=long&pmid=25869128 |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-10 |
| Num Groups | 4 |
| Total Subjects | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 32 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000156 |
| Project DOI: | doi: 10.21228/M8XS3B |
| Project Title: | Bone Marrow Studies |
| Project Summary: | Assessing if there is a difference between groups |
| Institute: | University of Michigan |
| Department: | Pedatric Endocrinology |
| Laboratory: | Singer Lab |
| Last Name: | Singer |
| First Name: | Kanakadurga |
| Address: | Ann Arbor, MI |
| Email: | ksinger@umich.edu |
| Phone: | 734-764-5175 |
Subject:
| Subject ID: | SU000220 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet (Normal or High Fat) | KW |
|---|---|---|---|
| SA010054 | S00015382 | HF | N |
| SA010055 | S00015383 | HF | N |
| SA010056 | S00015381 | HF | N |
| SA010057 | S00015384 | HF | N |
| SA010058 | S00015392 | HF | Y |
| SA010059 | S00015389 | HF | Y |
| SA010060 | S00015391 | HF | Y |
| SA010061 | S00015390 | HF | Y |
| SA010062 | S00015380 | N | N |
| SA010063 | S00015377 | N | N |
| SA010064 | S00015379 | N | N |
| SA010065 | S00015378 | N | N |
| SA010066 | S00015386 | N | Y |
| SA010067 | S00015385 | N | Y |
| SA010068 | S00015387 | N | Y |
| SA010069 | S00015388 | N | Y |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000208 |
| Collection Summary: | - |
| Sample Type: | Bone Marrow |
Treatment:
| Treatment ID: | TR000228 |
Sample Preparation:
| Sampleprep ID: | SP000222 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx |
Combined analysis:
| Analysis ID | AN000303 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nM (norm to 100ug/ml cell protein) |
Chromatography:
| Chromatography ID: | CH000224 |
| Methods ID: | AQM051 |
| Methods Filename: | QM-001-Xbrdg2mm-Acr+CoA-18min.m.zip |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000252 |
| Analysis ID: | AN000303 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | QM-001-Xbrdg2mm-Acr+CoA-18min.m.zip |
