Summary of Study ST000209

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000176. The data can be accessed directly via it's Project DOI: 10.21228/M80K5N This work is supported by NIH grant, U2C- DK119886.

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Study IDST000209
Study TitleGlycolysis/TCA/Nucleotide_Wt_KO_TimeCourse_LPS
Study SummaryWild type and AMPK KO bone marrow derived macrophage were stimulated with LPS (100ng/ml) for various time period and plates were frozen in liquid nitrogen after washing with sodium acetate (200uM) solution and kept in deep freezer for further analysis.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-11
Num Groups12
Total Subjects48
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size988 M
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M80K5N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000176
Project DOI:doi: 10.21228/M80K5N
Project Title:Wt and KO macrophage studies
Project Summary:Temperol metabolites profile of Wt and KO macrophage in response to LPS
Institute:Henry Ford Health System
Department:Neurology
Laboratory:Giri Lab
Last Name:Giri
First Name:Shailendra
Address:Detroit, MI
Email:sgiri1@hfhs.org
Phone:313-916-7725

Subject:

Subject ID:SU000228
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id time course Cell type Treatment
SA010358S000182210h KO macrophage untreated
SA010359S000182190h KO macrophage untreated
SA010360S000182220h KO macrophage untreated
SA010361S000182200h KO macrophage untreated
SA010362S000181980h Wt macrophage untreated
SA010363S000181960h Wt macrophage untreated
SA010364S000181950h Wt macrophage untreated
SA010365S000181970h Wt macrophage untreated
SA010366S0001823112h KO macrophage LPS
SA010367S0001823412h KO macrophage LPS
SA010368S0001823312h KO macrophage LPS
SA010369S0001823212h KO macrophage LPS
SA010370S0001821012h Wt macrophage LPS
SA010371S0001820912h Wt macrophage LPS
SA010372S0001820812h Wt macrophage LPS
SA010373S0001820712h Wt macrophage LPS
SA010374S0001823718h KO macrophage LPS
SA010375S0001823818h KO macrophage LPS
SA010376S0001823518h KO macrophage LPS
SA010377S0001823618h KO macrophage LPS
SA010378S0001821418h Wt macrophage LPS
SA010379S0001821218h Wt macrophage LPS
SA010380S0001821118h Wt macrophage LPS
SA010381S0001821318h Wt macrophage LPS
SA010382S0001824224h KO macrophage LPS
SA010383S0001824124h KO macrophage LPS
SA010384S0001824024h KO macrophage LPS
SA010385S0001823924h KO macrophage LPS
SA010386S0001821824h Wt macrophage LPS
SA010387S0001821524h Wt macrophage LPS
SA010388S0001821724h Wt macrophage LPS
SA010389S0001821624h Wt macrophage LPS
SA010390S000182263h KO macrophage LPS
SA010391S000182243h KO macrophage LPS
SA010392S000182233h KO macrophage LPS
SA010393S000182253h KO macrophage LPS
SA010394S000181993h Wt macrophage LPS
SA010395S000182023h Wt macrophage LPS
SA010396S000182003h Wt macrophage LPS
SA010397S000182013h Wt macrophage LPS
SA010398S000182306h KO macrophage LPS
SA010399S000182276h KO macrophage LPS
SA010400S000182296h KO macrophage LPS
SA010401S000182286h KO macrophage LPS
SA010402S000182056h Wt macrophage LPS
SA010403S000182066h Wt macrophage LPS
SA010404S000182046h Wt macrophage LPS
SA010405S000182036h Wt macrophage LPS
Showing results 1 to 48 of 48

Collection:

Collection ID:CO000216
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000236

Sample Preparation:

Sampleprep ID:SP000230
Sampleprep Summary:-
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000311
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units pmol/µg protein

Chromatography:

Chromatography ID:CH000232
Methods ID:AQM021
Methods Filename:QTOF-002-HILIC-35min-1mm_bottompheater.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000260
Analysis ID:AN000311
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm_bottompheater.m.zip
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