Summary of Study ST000212

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000178. The data can be accessed directly via it's Project DOI: 10.21228/M8VS3Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000212
Study TitleMurine gut bile acid analysis
Study TypeChanges to bile acids in the murine gut after antibiotics Bile acid analysis (colon contents/plasma)
Study SummarySamples were collected at the time of necropsy. Contents from the lumen of the gastrointestinal tract of C57BL/6 mice were collected, flash frozen and stored in the -80C until processing for metabolomics assay. C57BL/6 mice treated with cefoperazone for 5 days with two days of water (n=5) were harvested along with non-antibiotic treated mice (n=5). Contents from the small intesine (duodenum, jejunum and ileum), the large intestine (cecum, colon) and stool were collected for targeted bile acid analysis. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333439/
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-12
Num Groups12
Total Subjects60
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size32 M
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VS3Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000178
Project DOI:doi: 10.21228/M8VS3Q
Project Title:Bile Acid Studies in Murine Gut
Project Summary:Targeted bile acid analysis throughout the murine GI tract before and after antibiotics
Institute:University of Michigan
Department:Int Med-Infectious Diseases
Laboratory:Theriot Lab
Last Name:Theriot
First Name:Casey
Address:Ann Arbor, MI
Email:caseythe@umich.edu
Phone:734-763-4133

Subject:

Subject ID:SU000231
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GI tract location treatment
SA010429S00012887cecum cefoperazone
SA010430S00012884cecum cefoperazone
SA010431S00012885cecum cefoperazone
SA010432S00012886cecum cefoperazone
SA010433S00012883cecum cefoperazone
SA010434S00012914cecum vehicle
SA010435S00012917cecum vehicle
SA010436S00012916cecum vehicle
SA010437S00012915cecum vehicle
SA010438S00012913cecum vehicle
SA010439S00012891colon cefoperazone
SA010440S00012890colon cefoperazone
SA010441S00012889colon cefoperazone
SA010442S00012888colon cefoperazone
SA010443S00012892colon cefoperazone
SA010444S00012922colon vehicle
SA010445S00012919colon vehicle
SA010446S00012920colon vehicle
SA010447S00012921colon vehicle
SA010448S00012918colon vehicle
SA010449S00012868duodenum cefoperazone
SA010450S00012872duodenum cefoperazone
SA010451S00012869duodenum cefoperazone
SA010452S00012870duodenum cefoperazone
SA010453S00012871duodenum cefoperazone
SA010454S00012901duodenum vehicle
SA010455S00012902duodenum vehicle
SA010456S00012899duodenum vehicle
SA010457S00012898duodenum vehicle
SA010458S00012900duodenum vehicle
SA010459S00012880ileum cefoperazone
SA010460S00012878ileum cefoperazone
SA010461S00012881ileum cefoperazone
SA010462S00012879ileum cefoperazone
SA010463S00012882ileum cefoperazone
SA010464S00012909ileum vehicle
SA010465S00012910ileum vehicle
SA010466S00012911ileum vehicle
SA010467S00012908ileum vehicle
SA010468S00012912ileum vehicle
SA010469S00012874jejunum cefoperazone
SA010470S00012876jejunum cefoperazone
SA010471S00012873jejunum cefoperazone
SA010472S00012875jejunum cefoperazone
SA010473S00012877jejunum cefoperazone
SA010474S00012907jejunum vehicle
SA010475S00012903jejunum vehicle
SA010476S00012904jejunum vehicle
SA010477S00012905jejunum vehicle
SA010478S00012906jejunum vehicle
SA010479S00012897stool cefoperazone
SA010480S00012896stool cefoperazone
SA010481S00012893stool cefoperazone
SA010482S00012894stool cefoperazone
SA010483S00012895stool cefoperazone
SA010484S00012927stool vehicle
SA010485S00012926stool vehicle
SA010486S00012923stool vehicle
SA010487S00012924stool vehicle
SA010488S00012925stool vehicle
Showing results 1 to 60 of 60

Collection:

Collection ID:CO000219
Collection Summary:-
Sample Type:Cecum

Treatment:

Treatment ID:TR000239

Sample Preparation:

Sampleprep ID:SP000233
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000314
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units µg/100mg sample

Chromatography:

Chromatography ID:CH000235
Methods ID:AQM040
Methods Filename:2014-03-27-Bile_acid-full-ESN-insert.m.zip

MS:

MS ID:MS000263
Analysis ID:AN000314
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:2014-03-27-Bile_acid-full-ESN-insert.m.zip
Processing Parameters File:EX00256-Bile-Acids-Quant-method.m.zip
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