Summary of Study ST000215
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000169. The data can be accessed directly via it's Project DOI: 10.21228/M8N88K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000215 |
| Study Title | Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO GTT 1 mice |
| Study Type | 13C mass isotopomer analysis (flux studies) |
| Study Summary | Mice were fasted for 18 hr overnight then sacrificed or treated with 13C-U-glucose (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately freeze-clamped and stored in liquid N2 and then at -80 C. Wild type (IR and IR/FoxO1 floxed) mice were sacrificed after fasting and 1 hr post-glucose treatment. Liver-specific insulin receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO) mice were sacrificed 1 hr post glucose treatment. http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-12 |
| Num Groups | 5 |
| Total Subjects | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 2.5 G |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-12-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000169 |
| Project DOI: | doi: 10.21228/M8N88K |
| Project Title: | Regulation of hepatic metabolism |
| Project Summary: | Examine metabolite in liver and plasma before and after 13C-glucose load in wild type, LIRKO and LIRFKO mice |
| Institute: | University of Chicago |
| Department: | Jesse Brown VA Medical Center |
| Laboratory: | Unterman Lab |
| Last Name: | Unterman |
| First Name: | Terry |
| Address: | Chicago, IL |
| Email: | unterman@uic.edu |
| Phone: | 847-778-0943 |
Subject:
| Subject ID: | SU000234 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | treatment | Strain |
|---|---|---|---|
| SA010568 | S00013145 | glucose | IRF floxed |
| SA010565 | S00013143 | glucose | IR floxed |
| SA010566 | S00013144 | glucose | IR floxed |
| SA010567 | S00013142 | glucose | IR floxed |
| SA010569 | S00013152 | glucose | LIRFKO |
| SA010570 | S00013154 | glucose | LIRFKO |
| SA010571 | S00013153 | glucose | LIRFKO |
| SA010572 | S00013155 | glucose | LIRFKO |
| SA010573 | S00013149 | glucose | LIRKO |
| SA010574 | S00013150 | glucose | LIRKO |
| SA010575 | S00013151 | glucose | LIRKO |
| SA010576 | S00013148 | glucose | LIRKO |
| SA010577 | S00013147 | glucose | LIRKO |
| SA010578 | S00013146 | glucose | LIRKO |
| SA010579 | S00013139 | none | IR floxed |
| SA010580 | S00013141 | none | IR floxed |
| SA010581 | S00013138 | none | IR floxed |
| SA010582 | S00013137 | none | IR floxed |
| SA010583 | S00013136 | none | IR floxed |
| SA010584 | S00013140 | none | IR floxed |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO000222 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000242 |
Sample Preparation:
| Sampleprep ID: | SP000236 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000317 | AN000318 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Area | Area |
Chromatography:
| Chromatography ID: | CH000238 |
| Methods ID: | AQM010 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001306 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000266 |
| Analysis ID: | AN000317 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
| Processing Parameters File: | EX00258-flux-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip |
| MS ID: | MS000267 |
| Analysis ID: | AN000318 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Processing Parameters File: | EX00258-flux-MassHunterQuant-GlyTCA-DataAnalysis-GCMS-Method.m.zip |
