Summary of Study ST000218
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000160. The data can be accessed directly via it's Project DOI: 10.21228/M8JG6Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000218 |
Study Title | Role of Microbiome in Psoriatic Arthritis (SCFA in PsA) |
Study Type | Short-chain fatty acid analysis |
Study Summary | To characterize the diversity and taxonomic relative abundance of the gut microbiota in patients with never-treated, recent-onset psoriatic arthritis (PsA). Methods High-throughput 16S ribosomal RNA pyrosequencing was utilized to compare the community composition of gut microbiota in patients with PsA (n = 16), patients with psoriasis of the skin (n = 15), and healthy, matched control subjects (n = 17). Samples were further assessed for the presence and levels of fecal and serum secretory IgA (sIgA), proinflammatory proteins, and fatty acids. Results The gut microbiota observed in patients with PsA and patients with skin psoriasis was less diverse when compared to that in healthy controls. This could be attributed to the reduced presence of several taxa. Samples from both patient groups showed a relative decrease in abundance of Coprococcus species, while samples from PsA patients were also characterized by a significant reduction in Akkermansia, Ruminococcus, and Pseudobutyrivibrio. Supernatants of fecal samples from PsA patients revealed an increase in sIgA levels and decrease in RANKL levels. Analysis of fatty acids revealed low fecal quantities of hexanoate and heptanoate in both patients with PsA and patients with psoriasis. Conclusion Patients with PsA and patients with skin psoriasis had a lower relative abundance of multiple intestinal bacteria. Although some genera were concomitantly decreased in both conditions, PsA samples had a lower abundance of reportedly beneficial taxa. This gut microbiota profile in PsA was similar to that previously described in patients with inflammatory bowel disease and was associated with changes in specific inflammatory proteins unique to this group, and distinct from that in patients with skin psoriasis and healthy controls. Thus, the role of the gut microbiome in the continuum of psoriasis–PsA pathogenesis and the associated immune response merits further study. Research is published: http://onlinelibrary.wiley.com/doi/10.1002/art.38892/full |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-08 |
Num Groups | 4 |
Total Subjects | 89 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 16 M |
Analysis Type Detail | GC-MS |
Release Date | 2015-12-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000160 |
Project DOI: | doi: 10.21228/M8JG6Z |
Project Title: | Hirschprung Associated Enterocolitis Studies |
Project Summary: | Assess for correlations between SCFA content and history of HAEC |
Institute: | University of Michigan |
Department: | Surgery |
Laboratory: | Teitelbaum Lab |
Last Name: | Teitelbaum |
First Name: | Daniel |
Address: | Ann Arbor, MI |
Email: | dttbm@umich.edu |
Phone: | 734-936-8464 |
Subject:
Subject ID: | SU000237 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Disease Group |
---|---|---|
SA010619 | S00013315 | HLT |
SA010620 | S00013314 | HLT |
SA010621 | S00013313 | HLT |
SA010622 | S00013316 | HLT |
SA010623 | S00013318 | HLT |
SA010624 | S00013304 | HLT |
SA010625 | S00013319 | HLT |
SA010626 | S00013312 | HLT |
SA010627 | S00013317 | HLT |
SA010628 | S00013305 | HLT |
SA010629 | S00013311 | HLT |
SA010630 | S00013307 | HLT |
SA010631 | S00013306 | HLT |
SA010632 | S00013309 | HLT |
SA010633 | S00013310 | HLT |
SA010634 | S00013308 | HLT |
SA010635 | S00013375 | NORA |
SA010636 | S00013374 | NORA |
SA010637 | S00013376 | NORA |
SA010638 | S00013377 | NORA |
SA010639 | S00013373 | NORA |
SA010640 | S00013378 | NORA |
SA010641 | S00013369 | NORA |
SA010642 | S00013379 | NORA |
SA010643 | S00013368 | NORA |
SA010644 | S00013370 | NORA |
SA010645 | S00013371 | NORA |
SA010646 | S00013372 | NORA |
SA010647 | S00013383 | NORA |
SA010648 | S00013389 | NORA |
SA010649 | S00013388 | NORA |
SA010650 | S00013390 | NORA |
SA010651 | S00013380 | NORA |
SA010652 | S00013392 | NORA |
SA010653 | S00013387 | NORA |
SA010654 | S00013391 | NORA |
SA010655 | S00013381 | NORA |
SA010656 | S00013386 | NORA |
SA010657 | S00013382 | NORA |
SA010658 | S00013384 | NORA |
SA010659 | S00013385 | NORA |
SA010660 | S00013361 | Ps |
SA010661 | S00013360 | Ps |
SA010662 | S00013362 | Ps |
SA010663 | S00013359 | Ps |
SA010664 | S00013358 | Ps |
SA010665 | S00013365 | Ps |
SA010666 | S00013357 | Ps |
SA010667 | S00013367 | Ps |
SA010668 | S00013366 | Ps |
SA010669 | S00013364 | Ps |
SA010670 | S00013363 | Ps |
SA010671 | S00013348 | Ps |
SA010672 | S00013347 | Ps |
SA010673 | S00013349 | Ps |
SA010674 | S00013346 | Ps |
SA010675 | S00013345 | Ps |
SA010676 | S00013356 | Ps |
SA010677 | S00013350 | Ps |
SA010678 | S00013344 | Ps |
SA010679 | S00013351 | Ps |
SA010680 | S00013355 | Ps |
SA010681 | S00013354 | Ps |
SA010682 | S00013352 | Ps |
SA010683 | S00013353 | Ps |
SA010684 | S00013327 | PsA |
SA010685 | S00013326 | PsA |
SA010686 | S00013329 | PsA |
SA010687 | S00013330 | PsA |
SA010688 | S00013325 | PsA |
SA010689 | S00013328 | PsA |
SA010690 | S00013323 | PsA |
SA010691 | S00013331 | PsA |
SA010692 | S00013320 | PsA |
SA010693 | S00013321 | PsA |
SA010694 | S00013322 | PsA |
SA010695 | S00013324 | PsA |
SA010696 | S00013338 | PsA |
SA010697 | S00013340 | PsA |
SA010698 | S00013341 | PsA |
SA010699 | S00013342 | PsA |
SA010700 | S00013343 | PsA |
SA010701 | S00013339 | PsA |
SA010702 | S00013337 | PsA |
SA010703 | S00013333 | PsA |
SA010704 | S00013334 | PsA |
SA010705 | S00013335 | PsA |
SA010706 | S00013336 | PsA |
SA010707 | S00013332 | PsA |
Showing results 1 to 89 of 89 |
Collection:
Collection ID: | CO000225 |
Collection Summary: | - |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR000245 |
Sample Preparation:
Sampleprep ID: | SP000239 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | SCFA_analysis_protocol-2015-03-10.docx |
Combined analysis:
Analysis ID | AN000322 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | |
Column | Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975C |
Ion Mode | POSITIVE |
Units | pmol/mg sample dry weight |
Chromatography:
Chromatography ID: | CH000243 |
Methods ID: | AQM030 |
Methods Filename: | WAXPLUS_100-200C_2_SIM.M.txt |
Column Name: | Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS000271 |
Analysis ID: | AN000322 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | WAXPLUS_100-200C_2_SIM.M.txt |
Processing Parameters File: | EX00263-SCFA-Quant-methods.m.zip |