Summary of Study ST000228
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000185. The data can be accessed directly via it's Project DOI: 10.21228/M8BS32 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000228 |
| Study Title | Analysis of Dried Blood Spots by MALDI-MS |
| Study Type | Analysis of Dried Blood Spots by MALDI-MS |
| Study Summary | Concentrations of cocaine (5,3,2,1.5,1,0.75,0.5,0.25,0.125, 0.062, and 0.031 ppm) were spiked into whole human blood for MALDI-MS analysis. A 10 uL volume of blood/analyte mixture was allowed to dry on Whatman Grade 2 filter paper. A 1ppm concentration Cocaine-d3 was used as an internal standard. Parameters of the MALDI system were optimized, including laser energy, number of laser shots, and matrix. The internal standard was added on top of the dried blood spot sample. Wide isolation tandem MS was used to generate a calibration curve. |
| Institute | University of Florida |
| Department | Chemistry |
| Laboratory | Chemistry Lab Building |
| Last Name | Dhummakupt |
| First Name | Elizabeth |
| Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| emuffly@ufl.edu | |
| Phone | 352-392-0515 |
| Submit Date | 2015-07-16 |
| Num Groups | 11 |
| Total Subjects | 154 |
| Study Comments | 11 concentrations were run as 7 samples/concentration for 2 days |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 128 MB |
| Analysis Type Detail | MALDI-MS |
| Release Date | 2017-10-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000185 |
| Project DOI: | doi: 10.21228/M8BS32 |
| Project Title: | Direct Analysis of Dried Blood Spots by Mass Spectrometry |
| Project Type: | Direct analysis methods, dried blood spots, MALDI, paper spray |
| Project Summary: | The direct analysis methods of MALDI and paper spray ionization mass spectrometry were used to analyze dried blood spots without the need for punching out or extracting the samples. Ionization and instrument parameters were optimized, and a quantification method was developed for both techniques utilizing internal standards and a wide isolation mass window. Additionally, the continuous addition of solvent to the paper spray ionization setup was explored, as well as the use of a solvent gradient for potential sample clean-up. |
| Institute: | University of Florida |
| Department: | Chemistry |
| Laboratory: | Chemistry Lab Building |
| Last Name: | Yost |
| First Name: | Richard |
| Address: | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| Email: | ryost@chem.ufl.edu |
| Phone: | 352-392-0515 |
| Funding Source: | SECIM U24 DK097209 |
Subject:
| Subject ID: | SU000247 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cocaine conentration (ppb) |
|---|---|---|
| SA010827 | 1ppmCoc_1ppmCoc_intensity_AVG | 1000 |
| SA010823 | 125ppbCoc_1ppmCoc_intensity_AVG | 125 |
| SA010828 | 1.5ppmCoc_1ppmCoc_intensity_AVG | 1500 |
| SA010829 | 2ppmCoc_1ppmCoc_intensity_AVG | 2000 |
| SA010824 | 250ppbCoc_1ppmCoc_intensity_AVG | 250 |
| SA010830 | 3ppmCoc_1ppmCoc_intensity_AVG | 3000 |
| SA010821 | 31ppbCoc_1ppmCoc_intensity_AVG | 31 |
| SA010825 | 500ppbCoc_1ppmCoc_intensity_AVG | 500 |
| SA010831 | 5ppmCoc_1ppmCoc_intensity_AVG | 5000 |
| SA010822 | 62ppbCoc_1ppmCoc_intensity_AVG | 62 |
| SA010826 | 750ppbCoc_1ppmCoc_intensity_AVG | 750 |
| Showing results 1 to 11 of 11 |
Collection:
| Collection ID: | CO000236 |
| Collection Summary: | - |
| Sample Type: | Blood |
| Volumeoramount Collected: | 20 mL |
| Storage Conditions: | Stored at 4 C |
| Additives: | NaEDTA |
Treatment:
| Treatment ID: | TR000256 |
| Treatment Protocol Comments: | Whole blood stored at 4 C; in liquid form, blood samples viable for 6 weeks |
Sample Preparation:
| Sampleprep ID: | SP000250 |
| Sampleprep Summary: | Addition of cocaine to blood samples |
| Sample Spiking: | Unlabled cocaine was spiked into all samples. The deuterated cocaine was added on top of the dried spot. |
Combined analysis:
| Analysis ID | AN000341 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | |
| Column | |
| MS Type | MALDI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo LTQ XL |
| Ion Mode | POSITIVE |
| Units | Avg Intensity |
Chromatography:
| Chromatography ID: | CH000255 |
MS:
| MS ID: | MS000287 |
| Analysis ID: | AN000341 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Single quadrupole |
| MS Type: | MALDI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 85 |
| Collision Energy: | N2 |
| Ion Spray Voltage: | MALDI |
| Cdl Temperature: | .raw |
| It Side Octopoles Bias Voltage: | N2 gas laser, 10 uJ laser energy, 3 laser shots |
| Laser: | 2,5-DIHYDROXYBENZOIC ACID |
| Matrix: | Meinhard |
| Sample Dripping: | 80-500 |
| Acquisition Parameters File: | LizTuneFile_WideIsolation_forBlood |
