Summary of Study ST000233

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000233
Study TitleMetabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice (Part II)
Study TypeTime course
Study Summary12 C57BL/6J male mice were acclimatized for 1 week and then divided into two groups and trained to eat dough pills. One group was control and the other was exposed to TCDF through the dough pills. The mice were treated for 5 days at a dosage .15μg of TCDF for a final concentration of 5μg/kg body weight. Also looked at were AHR-/- mice and they were treated the same way as the C57BL/6J mice.
Institute
Pennsylvania State University
DepartmentDepartment of Veterinary and Biomedical Sciences
Last NamePatterson
First NameAndrew
Address101 Life Sciences, University Park, State college, 16802
Emailrgn5011@psu.edu
Submit Date2015-07-07
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size256 MB
Analysis Type DetailGC-MS
Release Date2015-08-14
Release Version1
Andrew Patterson Andrew Patterson
https://dx.doi.org/10.21228/M82P4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000120
Project DOI:doi: 10.21228/M82P4X
Project Title:Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice
Institute:Pennsylvania State University
Department:Molecular Toxicology
Last Name:Patterson
First Name:Andrew
Email:adp117@PSU.EDU; RGN5011@psu.edu

Subject:

Subject ID:SU000252
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment
SA010885101AHR -/- Control
SA010886105AHR -/- Control
SA010887104AHR -/- Control
SA010888102AHR -/- Control
SA010889103AHR -/- Control
SA010890204AHR -/- TCDF Treatment
SA010891203AHR -/- TCDF Treatment
SA010892205AHR -/- TCDF Treatment
SA010893202AHR -/- TCDF Treatment
SA010894201AHR -/- TCDF Treatment
SA010895303WT Control
SA010896305WT Control
SA010897304WT Control
SA010898302WT Control
SA010899301WT Control
SA010900405WT TCDF Treatment
SA010901403WT TCDF Treatment
SA010902401WT TCDF Treatment
SA010903402WT TCDF Treatment
Showing results 1 to 19 of 19

Collection:

Collection ID:CO000242
Collection Summary:Feces and urine were collected every other day. Then Liver, blood and intestinal tissue were collected after death
Sample Type:Feces, Urine, Liver, Blood, Intestinal tissue

Treatment:

Treatment ID:TR000262

Sample Preparation:

Sampleprep ID:SP000256
Sampleprep Protocol Filename:GC_Sample_Prep.docx
Processing Method:Homogenization
Extraction Method:Methanol-Chloroform
Extract Enrichment:centerfugation
Extract Cleanup:bottom layer was dried with N2 gas, resuspended in MEOH/HCL and then hexane and saline, top layer was collected
Sample Resuspension:resuspended in hexane
Sample Spiking:C15:0 free acid and methy ester of C17:0 at 5 ?L
Organ:Liver

Combined analysis:

Analysis ID AN000349 AN000350
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type EI EI
MS instrument type Single quadrupole Single quadrupole
MS instrument name Agilent 5975C Agilent 5975C
Ion Mode POSITIVE POSITIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000260
Chromatography Type:GC

MS:

MS ID:MS000295
Analysis ID:AN000349
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
  
MS ID:MS000296
Analysis ID:AN000350
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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