Summary of Study ST000239
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000192. The data can be accessed directly via it's Project DOI: 10.21228/M8K88Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000239 |
| Study Title | Sexual antagonism in exuded non-volatile metabolites in C. purpureus |
| Study Type | male - female |
| Study Summary | The experimental approach seeks to test for sexual dimorphism in exuded non-volatile metabolites in C. purpureus. The proposed research is creative and original both in its inter-disciplinary approach and its use of a biochemically tractable phenotype to develop a much-needed link between natural selection for sexual dimorphism and the molecular targets of that selection pressure. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | McDaniel |
| First Name | Stuart |
| stuartmcdaniel@ufl.edu | |
| Submit Date | 2015-07-01 |
| Total Subjects | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000192 |
| Project DOI: | doi: 10.21228/M8K88Z |
| Project Title: | Sexual antagonism in exuded non-volatile metabolites in C. purpureus |
| Project Summary: | To study the mechanisms responsible for the evolution of sexual dimorphism by identifying sex differences in metabolite production. The PIs recently demonstrated that female mosses produce large numbers of volatile organic compounds (VOCs) that specifically attract microarthropods, and that the presence of these insects increases fertilization rates (ie, a previously unknown fertilization syndrome). Male mosses produce many fewer VOCs, suggesting that VOC production may be costly. Collectively, these results suggest that alleles controlling VOC production may experience ongoing sexual antagonism. Here the PIs propose to generate pilot data to rigorously establish a link between dimorphisms in metabolite profile and its underlying genetic bases in the moss Ceratodon purpureus and related species. |
| Institute: | University of Florida;Pennsylvania State University |
| Department: | Biology |
| Last Name: | McDaniel |
| First Name: | Stuart |
| Email: | stuartmcdaniel@ufl.edu |
| Funding Source: | Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 |
Subject:
| Subject ID: | SU000259 |
| Subject Type: | Plant |
| Subject Species: | Ceratodon purpureus |
| Taxonomy ID: | 3225 |
| Species Group: | Plants |
Factors:
Subject type: Plant; Subject species: Ceratodon purpureus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Gender |
|---|---|---|
| SA011092 | I180f | Female |
| SA011093 | H140f | Female |
| SA011094 | B150f | Female |
| SA011095 | O120f | Female |
| SA011096 | N170f | Female |
| SA011097 | G150f | Female |
| SA011098 | L100f | Female |
| SA011099 | E270f | Female |
| SA011100 | M280f | Female |
| SA011101 | F140f | Female |
| SA011102 | M270m | Male |
| SA011103 | G100m | Male |
| SA011104 | H150m | Male |
| SA011105 | I190m | Male |
| SA011106 | E280m | Male |
| SA011107 | B190m | Male |
| SA011108 | F110m | Male |
| SA011109 | L120m | Male |
| SA011110 | O110m | Male |
| SA011111 | N110m | Male |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO000249 |
| Collection Summary: | Flash frozen and lyophilzed |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR000269 |
| Treatment Summary: | standard growth conditions - 6 day old protonema |
Sample Preparation:
| Sampleprep ID: | SP000263 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol.pdf |
Combined analysis:
| Analysis ID | AN000369 | AN000370 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000268 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000315 |
| Analysis ID: | AN000369 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000316 |
| Analysis ID: | AN000370 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
