Summary of Study ST000240
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000193. The data can be accessed directly via it's Project DOI: 10.21228/M8FK5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000240 |
| Study Title | Global LC-MS of Ozone Stress in Maize: GLCMS |
| Study Type | ozone treatment |
| Study Summary | Global LC-MS of maize leaf samples collected from plants grown at ambient and elevated ozone concentrations in replicated field experiment |
| Institute | University of Florida |
| Department | Southeastern Center for Integrated Metabolomics (SECIM) |
| Last Name | Ainsworth |
| First Name | Elizabeth |
| ainswort@illinois.edu | |
| Submit Date | 2015-02-24 |
| Num Groups | 2 |
| Total Subjects | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000193 |
| Project DOI: | doi: 10.21228/M8FK5P |
| Project Title: | Global LC-MS of Ozone Stress in Maize: GLCMS |
| Project Summary: | Oxidative stress arising from O3 exposure is reducing potential maize yields by up to 10%. Yield losses to O3 are projected to as much as double by mid-century, and there is thought to be little potential for adaptation to rising O3 concentrations ([O3]) through altered crop management practices. Therefore, the only solution to current and future O3-induced yield loss is development of O3 tolerant maize through breeding and/or biotechnology. In this project, we will investigate the metabolite profiles that underpin O3 sensitivity in maize. |
| Institute: | University of Illinois Urbana-Champaign |
| Department: | Plant Biology |
| Last Name: | Ainsworth |
| First Name: | Elizabeth |
| Address: | 1201 W. Gregory Dr., Urbana, IL 61801 |
| Email: | ainswort@illinois.edu |
| Phone: | (217) 265-9887 |
| Funding Source: | Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 |
Subject:
| Subject ID: | SU000260 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Genotype Strain: | inbred line B73 |
| Species Group: | Plants |
Factors:
Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)
| mb_sample_id | local_sample_id | Ring No | Treatment |
|---|---|---|---|
| SA011112 | R10 B73 41021 | 10 | ambient |
| SA011113 | R10 B73 41023 | 10 | ambient |
| SA011114 | R10 B73 41044 | 10 | ambient |
| SA011115 | R13 B73 41344 | 13 | ozone |
| SA011116 | R13 B73 41321 | 13 | ozone |
| SA011117 | R13 B73 41324 | 13 | ozone |
| SA011118 | R14 B73 41424 | 14 | ambient |
| SA011119 | R14 B73 41421 | 14 | ambient |
| SA011120 | R14 B73 41444 | 14 | ambient |
| SA011121 | R1 B73 40104 | 1 | ambient |
| SA011122 | R1 B73 40143 | 1 | ambient |
| SA011123 | R1 B73 40122 | 1 | ambient |
| SA011124 | R2 B73 40243 | 2 | ozone |
| SA011125 | R2 B73 40222 | 2 | ozone |
| SA011126 | R2 B73 40204 | 2 | ozone |
| SA011127 | R5 B73 40525 | 5 | ambient |
| SA011128 | R5 B73 40542 | 5 | ambient |
| SA011129 | R5 B73 40520 | 5 | ambient |
| SA011130 | R6 B73 40642 | 6 | ozone |
| SA011131 | R6 B73 40625 | 6 | ozone |
| SA011132 | R6 B73 40620 | 6 | ozone |
| SA011133 | R9 B73 40944 | 9 | ozone |
| SA011134 | R9 B73 40921 | 9 | ozone |
| SA011135 | R9 B73 40923 | 9 | ozone |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO000250 |
| Collection Summary: | - |
| Collection Protocol Filename: | Collection_summary_ainsworth.txt |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR000270 |
| Treatment Summary: | Maize (inbred line B73) grown at ambient (~40 ppb) and elevated O3 concentrations ([O3]; 100 ppb). |
| Treatment Protocol Filename: | Treatment_summary_ainsworth.txt |
Sample Preparation:
| Sampleprep ID: | SP000264 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
| Sampleprep Protocol Comments: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
| Analysis ID | AN000371 | AN000372 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000269 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000317 |
| Analysis ID: | AN000371 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000318 |
| Analysis ID: | AN000372 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
