Summary of Study ST000243
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000196. The data can be accessed directly via it's Project DOI: 10.21228/M82C7Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000243 |
Study Title | Metabolomic-based investigation on the effects of antifungal agents in Candida albicans |
Study Type | in vitro study/drug dosage |
Study Summary | Our aim is to determine the metabolic effects of increasing doses of an antifungal agent on C. albicans metabolism (untargeted, steady state metabolomics). We will culture in vitro Candida cells to the mid-logarithmic growth in liquid media (RPMI-1640) at 37°C and then inoculate biological replicates (1ml) onto 22mm nitrocellulose filters under vocuum filtration in sterile conditions. Subsequently, isolates will be cultivated to midlogarithmic phase of growth on the same agar (RPMI-1640) to which the antifungal agent has been added at a range of concentrations to achieve doses equivalent to 0 MIC (no drug), 0.0625 MIC, 0.125 MIC, 0.25 MIC, 0.5 MIC and 1.0 MIC at 37°C. At mid-logarithmic phase of growth (12h) replicates will be metabolically quenched by immersion into a solvent mixture of 40% acetonitrile: 40% methanol: 20% water precooled at -40°C. The resulting quenched isolate/solvent mixtrue will be mechanically lysed by bead-beating with 0.1mm Zirconia beads in a tissue homogenizer and then centrifuged to seperate out cell wall components. Supernatants will be removed and stored at -80°C until they will be sent to SECIM facility. |
Institute | University of Florida |
Department | SECIM |
Last Name | Katragkou |
First Name | Aspasia |
ask2012@med.cornell.edu | |
Submit Date | 2015-02-10 |
Num Groups | 96 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2016-09-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000196 |
Project DOI: | doi: 10.21228/M82C7Z |
Project Title: | Metabolomic-based investigation on the effects of antifungal agents in Candida albicans |
Project Summary: | Our aim is to determine the metabolic effects of increasing doses of an antifungal agent on C. albicans metabolism (untargeted, steady state metabolomics). We will culture in vitro Candida cells to the mid-logarithmic growth in liquid media (RPMI-1640) at 37°C and then inoculate biological replicates (1ml) onto 22mm nitrocellulose filters under vocuum filtration in sterile conditions. Subsequently, isolates will be cultivated to midlogarithmic phase of growth on the same agar (RPMI-1640) to which the antifungal agent has been added at a range of concentrations to achieve doses equivalent to 0 MIC (no drug), 0.0625 MIC, 0.125 MIC, 0.25 MIC, 0.5 MIC and 1.0 MIC at 37°C. At mid-logarithmic phase of growth (12h) replicates will be metabolically quenched by immersion into a solvent mixture of 40% acetonitrile: 40% methanol: 20% water precooled at -40°C. The resulting quenched isolate/solvent mixtrue will be mechanically lysed by bead-beating with 0.1mm Zirconia beads in a tissue homogenizer and then centrifuged to seperate out cell wall components. Supernatants will be removed and stored at -80°C until they will be sent to SECIM facility. |
Institute: | Weill Cornell Medicine |
Department: | Medicine/Infectious Diseases |
Last Name: | Walsh |
First Name: | Thomas |
Email: | thw2003@med.cornell.edu |
Funding Source: | Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 |
Subject:
Subject ID: | SU000263 |
Subject Type: | Yeast cells |
Subject Species: | Candida albicans |
Taxonomy ID: | 5476 |
Genotype Strain: | SC5314 |
Species Group: | Yeast |
Factors:
Subject type: Yeast cells; Subject species: Candida albicans (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | Experiment |
---|---|---|---|
SA011169 | EXP_1_0.0625MIC_3 | 0.0625MIC | 1 |
SA011170 | EXP_1_0.0625MIC_2 | 0.0625MIC | 1 |
SA011171 | EXP_1_0.0625MIC_4 | 0.0625MIC | 1 |
SA011172 | EXP_1_0.0625MIC_5 | 0.0625MIC | 1 |
SA011173 | EXP_1_0.0625MIC_7 | 0.0625MIC | 1 |
SA011174 | EXP_1_0.0625MIC_6 | 0.0625MIC | 1 |
SA011175 | EXP_1_0.0625MIC_1 | 0.0625MIC | 1 |
SA011176 | EXP_1_0.0625MIC_8 | 0.0625MIC | 1 |
SA011177 | EXP_2_0.0625MIC_8 | 0.0625MIC | 2 |
SA011178 | EXP_2_0.0625MIC_1 | 0.0625MIC | 2 |
SA011179 | EXP_2_0.0625MIC_2 | 0.0625MIC | 2 |
SA011180 | EXP_2_0.0625MIC_6 | 0.0625MIC | 2 |
SA011181 | EXP_2_0.0625MIC_7 | 0.0625MIC | 2 |
SA011182 | EXP_2_0.0625MIC_3 | 0.0625MIC | 2 |
SA011183 | EXP_2_0.0625MIC_4 | 0.0625MIC | 2 |
SA011184 | EXP_2_0.0625MIC_5 | 0.0625MIC | 2 |
SA011185 | EXP_1_0.125MIC_6 | 0.125MIC | 1 |
SA011186 | EXP_1_0.125MIC_8 | 0.125MIC | 1 |
SA011187 | EXP_1_0.125MIC_5 | 0.125MIC | 1 |
SA011188 | EXP_1_0.125MIC_7 | 0.125MIC | 1 |
SA011189 | EXP_1_0.125MIC_4 | 0.125MIC | 1 |
SA011190 | EXP_1_0.125MIC_1 | 0.125MIC | 1 |
SA011191 | EXP_1_0.125MIC_2 | 0.125MIC | 1 |
SA011192 | EXP_1_0.125MIC_3 | 0.125MIC | 1 |
SA011193 | EXP_2_0.125MIC_6 | 0.125MIC | 2 |
SA011194 | EXP_2_0.125MIC_1 | 0.125MIC | 2 |
SA011195 | EXP_2_0.125MIC_5 | 0.125MIC | 2 |
SA011196 | EXP_2_0.125MIC_7 | 0.125MIC | 2 |
SA011197 | EXP_2_0.125MIC_3 | 0.125MIC | 2 |
SA011198 | EXP_2_0.125MIC_8 | 0.125MIC | 2 |
SA011199 | EXP_2_0.125MIC_2 | 0.125MIC | 2 |
SA011200 | EXP_2_0.125MIC_4 | 0.125MIC | 2 |
SA011201 | EXP_1_0.25MIC_8 | 0.25MIC | 1 |
SA011202 | EXP_1_0.25MIC_6 | 0.25MIC | 1 |
SA011203 | EXP_1_0.25MIC_7 | 0.25MIC | 1 |
SA011204 | EXP_1_0.25MIC_4 | 0.25MIC | 1 |
SA011205 | EXP_1_0.25MIC_5 | 0.25MIC | 1 |
SA011206 | EXP_1_0.25MIC_3 | 0.25MIC | 1 |
SA011207 | EXP_1_0.25MIC_2 | 0.25MIC | 1 |
SA011208 | EXP_1_0.25MIC_1 | 0.25MIC | 1 |
SA011209 | EXP_2_0.25MIC_4 | 0.25MIC | 2 |
SA011210 | EXP_2_0.25MIC_3 | 0.25MIC | 2 |
SA011211 | EXP_2_0.25MIC_2 | 0.25MIC | 2 |
SA011212 | EXP_2_0.25MIC_5 | 0.25MIC | 2 |
SA011213 | EXP_2_0.25MIC_1 | 0.25MIC | 2 |
SA011214 | EXP_2_0.25MIC_8 | 0.25MIC | 2 |
SA011215 | EXP_2_0.25MIC_7 | 0.25MIC | 2 |
SA011216 | EXP_2_0.25MIC_6 | 0.25MIC | 2 |
SA011217 | EXP_1_0.5MIC_6 | 0.5MIC | 1 |
SA011218 | EXP_1_0.5MIC_3 | 0.5MIC | 1 |
SA011219 | EXP_1_0.5MIC_7 | 0.5MIC | 1 |
SA011220 | EXP_1_0.5MIC_4 | 0.5MIC | 1 |
SA011221 | EXP_1_0.5MIC_5 | 0.5MIC | 1 |
SA011222 | EXP_1_0.5MIC_8 | 0.5MIC | 1 |
SA011223 | EXP_1_0.5MIC_1 | 0.5MIC | 1 |
SA011224 | EXP_1_0.5MIC_2 | 0.5MIC | 1 |
SA011225 | EXP_2_0.5MIC_6 | 0.5MIC | 2 |
SA011226 | EXP_2_0.5MIC_8 | 0.5MIC | 2 |
SA011227 | EXP_2_0.5MIC_7 | 0.5MIC | 2 |
SA011228 | EXP_2_0.5MIC_5 | 0.5MIC | 2 |
SA011229 | EXP_2_0.5MIC_3 | 0.5MIC | 2 |
SA011230 | EXP_2_0.5MIC_1 | 0.5MIC | 2 |
SA011231 | EXP_2_0.5MIC_2 | 0.5MIC | 2 |
SA011232 | EXP_2_0.5MIC_4 | 0.5MIC | 2 |
SA011233 | EXP_1_1.0MIC_8 | 1.0MIC | 1 |
SA011234 | EXP_1_1.0MIC_5 | 1.0MIC | 1 |
SA011235 | EXP_1_1.0MIC_6 | 1.0MIC | 1 |
SA011236 | EXP_1_1.0MIC_4 | 1.0MIC | 1 |
SA011237 | EXP_1_1.0MIC_3 | 1.0MIC | 1 |
SA011238 | EXP_1_1.0MIC_2 | 1.0MIC | 1 |
SA011239 | EXP_1_1.0MIC_7 | 1.0MIC | 1 |
SA011240 | EXP_1_1.0MIC_1 | 1.0MIC | 1 |
SA011241 | EXP_2_1.0MIC_3 | 1.0MIC | 2 |
SA011242 | EXP_2_1.0MIC_2 | 1.0MIC | 2 |
SA011243 | EXP_2_1.0MIC_1 | 1.0MIC | 2 |
SA011244 | EXP_2_1.0MIC_5 | 1.0MIC | 2 |
SA011245 | EXP_2_1.0MIC_4 | 1.0MIC | 2 |
SA011246 | EXP_2_1.0MIC_7 | 1.0MIC | 2 |
SA011247 | EXP_2_1.0MIC_8 | 1.0MIC | 2 |
SA011248 | EXP_2_1.0MIC_6 | 1.0MIC | 2 |
SA011249 | EXP_1_0MIC_4 | no drug | 1 |
SA011250 | EXP_1_0MIC_3 | no drug | 1 |
SA011251 | EXP_1_0MIC_2 | no drug | 1 |
SA011252 | EXP_1_0MIC_5 | no drug | 1 |
SA011253 | EXP_1_0MIC_6 | no drug | 1 |
SA011254 | EXP_1_0MIC_1 | no drug | 1 |
SA011255 | EXP_1_0MIC_8 | no drug | 1 |
SA011256 | EXP_1_0MIC_7 | no drug | 1 |
SA011257 | EXP_2_0MIC_3 | no drug | 2 |
SA011258 | EXP_2_0MIC_2 | no drug | 2 |
SA011259 | EXP_2_0MIC_4 | no drug | 2 |
SA011260 | EXP_2_0MIC_6 | no drug | 2 |
SA011261 | EXP_2_0MIC_8 | no drug | 2 |
SA011262 | EXP_2_0MIC_7 | no drug | 2 |
SA011263 | EXP_2_0MIC_1 | no drug | 2 |
SA011264 | EXP_2_0MIC_5 | no drug | 2 |
Showing results 1 to 96 of 96 |
Collection:
Collection ID: | CO000254 |
Collection Summary: | Culture supernatants (appx. 1 ml from each replicate) will be removed and stored at -80C |
Sample Type: | Cells |
Volumeoramount Collected: | 1 mL |
Storage Conditions: | -80C |
Treatment:
Treatment ID: | TR000274 |
Treatment Summary: | Candida cells grown to the mid-logarithmic phase will be exposed in biological replicates (X8) to the antifungal agent (micafungin) to a range of concentrations (sub-concentrations of the strain's MIC, Minimal Inhibitory Concentration) 0.0625MIC 0.125MIC 0.25MIC 0.5MIC 1.0MIC. No drug exposed Candida cells will serve as controls |
Sample Preparation:
Sampleprep ID: | SP000268 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Sample_Preparation_of_Cell_Lysates.pdf |
Combined analysis:
Analysis ID | AN000379 | AN000380 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000273 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000325 |
Analysis ID: | AN000379 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000326 |
Analysis ID: | AN000380 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |