Summary of Study ST000249

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000201. The data can be accessed directly via it's Project DOI: 10.21228/M8DK5C This work is supported by NIH grant, U2C- DK119886.


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Study IDST000249
Study TitleMeasurement of free amino acid (AA) in response to MYC
Study Typetreatment
Study SummaryThe proposed study will provide us information how the different AAs are affected by MYC. MYC is known to cause increased levels of glutamin due to import and synthesis, but we hypothesize that the overall pool of free AA in the cells is reduced, especially when cells are starved.
University of Florida
Last NameSchaub
First NameFranz
Submit Date2015-09-22
Num Groups2
Total Subjects6
Raw Data AvailableNo
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Franz Schaub Franz Schaub application/zip

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Project ID:PR000201
Project DOI:doi: 10.21228/M8DK5C
Project Title:Myc-directed suppression of autophagy provides therapeutic vulnerabilities targeting amino acid homeostasis
Project Type:Targeted LCMS Metabolomics
Project Summary:Hypothesis:MYC is suppressing the autophagy pathway which leads to impaired recycling of proteins and reduced free AA in the cells.
Institute:Moffitt Cancer Center
Department:Tumor Biology
Laboratory:John Cleveland
Last Name:Cleveland
First Name:John
Address:12902 Magnolia Dr,Tampa, FL 33612
Phone:813 745 3888
Funding Source:NIH


Subject ID:SU000269
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human


Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA01138448hr_TET_248 hour Tet treatment
SA01138548hr_TET_348 hour Tet treatment
SA01138648hr_TET_148 hour Tet treatment
SA01138748hr_NoTET_3No Tet treatment
SA01138848hr_NoTET_2No Tet treatment
SA01138948hr_NoTET_1No Tet treatment
Showing results 1 to 6 of 6


Collection ID:CO000260
Collection Summary:Cells were counted, washed with cold PBS and then flash-frozen in liquid N2
Sample Type:Cells
Volumeoramount Collected:1.00E7


Treatment ID:TR000280
Treatment Summary:Cells were treated with TET for 48 hrs (TET samples) and then washed and cultured without TET for 48hrs (noTET samples)

Sample Preparation:

Sampleprep ID:SP000274
Sampleprep Summary:Amino acid
Sampleprep Protocol Filename:SB_AA_Assay.pdf

Combined analysis:

Analysis ID AN000393
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um)
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Units nmol/million cells


Chromatography ID:CH000279
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase


MS ID:MS000337
Analysis ID:AN000393
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole