Summary of Study ST000250
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000202. The data can be accessed directly via it's Project DOI: 10.21228/M88W23 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000250 |
| Study Title | The Role of Obesity and Adipocytes in Immune Activation on Antiretroviral Therapy |
| Study Type | Observational, no intervention |
| Study Summary | Cross-sectional, three arms |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Koethe |
| First Name | John |
| Address | A2200-MCN, 1161 21st Ave South, Nashville, TN 37232-2582 |
| john.r.koethe@vanderbilt.edu | |
| Phone | 615-343-0533 |
| Submit Date | 2015-01-07 |
| Num Groups | 3 |
| Total Subjects | 100 patients, one sample per patient |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Thermo), d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000202 |
| Project DOI: | doi: 10.21228/M88W23 |
| Project Title: | The Role of Obesity and Adipocytes in Immune Activation on Antiretroviral Therapy |
| Project Type: | Clinical research |
| Project Summary: | The goal of this study is the use metabolomics to probe the role of BCAA metabolism in the apthogenisis of insulin-resistance associated with HIV-infection/ART. We will also quantify the plasma levels of 2-HBA in HIV-infected, ART -treated patients. These patients were substratified into obese or lean subjects |
| Institute: | Vanderbilt University |
| Department: | Division of Infectious Diseases |
| Last Name: | Koethe |
| First Name: | John |
| Address: | A2200-MCN, 1161 21st Ave South, Nashville, TN 37232-2582 |
| Email: | john.r.koethe@vanderbilt.edu |
| Phone: | 615-343-0533 |
| Funding Source: | National Institute of Allergy and Infectious Diseases |
Subject:
| Subject ID: | SU000270 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | HIV status | Obesity | Sex | Race |
|---|---|---|---|---|---|
| SA011390 | 523 | negative | Yes | F | B |
| SA011391 | 524 | negative | Yes | F | B |
| SA011392 | 520 | negative | Yes | F | B |
| SA011393 | 516 | negative | Yes | F | B |
| SA011394 | 509 | negative | Yes | F | B |
| SA011395 | 515 | negative | Yes | F | B |
| SA011396 | 527 | negative | Yes | F | B |
| SA011397 | 517 | negative | Yes | F | B |
| SA011398 | 514 | negative | Yes | F | W |
| SA011399 | 513 | negative | Yes | F | W |
| SA011400 | 505 | negative | Yes | F | W |
| SA011401 | 502 | negative | Yes | F | W |
| SA011402 | 534 | negative | Yes | F | W |
| SA011403 | 533 | negative | Yes | F | W |
| SA011404 | 521 | negative | Yes | F | W |
| SA011405 | 525 | negative | Yes | F | W |
| SA011406 | 503 | negative | Yes | F | W |
| SA011407 | 501 | negative | Yes | F | W |
| SA011408 | 512 | negative | Yes | M | B |
| SA011409 | 528 | negative | Yes | M | B |
| SA011410 | 531 | negative | Yes | M | B |
| SA011411 | 507 | negative | Yes | M | B |
| SA011412 | 510 | negative | Yes | M | W |
| SA011413 | 506 | negative | Yes | M | W |
| SA011414 | 511 | negative | Yes | M | W |
| SA011415 | 508 | negative | Yes | M | W |
| SA011416 | 519 | negative | Yes | M | W |
| SA011417 | 518 | negative | Yes | M | W |
| SA011418 | 530 | negative | Yes | M | W |
| SA011419 | 532 | negative | Yes | M | W |
| SA011420 | 42 | positive | No | F | B |
| SA011421 | 3 | positive | No | F | B |
| SA011422 | 41 | positive | No | F | B |
| SA011423 | 71 | positive | No | F | B |
| SA011424 | 23 | positive | No | F | B |
| SA011425 | 58 | positive | No | F | B |
| SA011426 | 24 | positive | No | F | B |
| SA011427 | 33 | positive | No | F | B |
| SA011428 | 34 | positive | No | F | B |
| SA011429 | 37 | positive | No | F | B |
| SA011430 | 60 | positive | No | F | W |
| SA011431 | 55 | positive | No | F | W |
| SA011432 | 61 | positive | No | F | W |
| SA011433 | 43 | positive | No | F | W |
| SA011434 | 30 | positive | No | M | B |
| SA011435 | 13 | positive | No | M | B |
| SA011436 | 35 | positive | No | M | B |
| SA011437 | 14 | positive | No | M | B |
| SA011438 | 4 | positive | No | M | B |
| SA011439 | 27 | positive | No | M | B |
| SA011440 | 1 | positive | No | M | O |
| SA011441 | 67 | positive | No | M | W |
| SA011442 | 57 | positive | No | M | W |
| SA011443 | 16 | positive | No | M | W |
| SA011444 | 54 | positive | No | M | W |
| SA011445 | 8 | positive | No | M | W |
| SA011446 | 36 | positive | No | M | W |
| SA011447 | 7 | positive | No | M | W |
| SA011448 | 26 | positive | No | M | W |
| SA011449 | 6 | positive | No | M | W |
| SA011450 | 31 | positive | No | M | W |
| SA011451 | 5 | positive | No | M | W |
| SA011452 | 17 | positive | Yes | F | B |
| SA011453 | 20 | positive | Yes | F | B |
| SA011454 | 32 | positive | Yes | F | B |
| SA011455 | 25 | positive | Yes | F | B |
| SA011456 | 74 | positive | Yes | F | B |
| SA011457 | 56 | positive | Yes | F | B |
| SA011458 | 64 | positive | Yes | F | B |
| SA011459 | 69 | positive | Yes | F | B |
| SA011460 | 77 | positive | Yes | F | B |
| SA011461 | 15 | positive | Yes | F | B |
| SA011462 | 10 | positive | Yes | F | B |
| SA011463 | 62 | positive | Yes | F | B |
| SA011464 | 22 | positive | Yes | F | W |
| SA011465 | 45 | positive | Yes | F | W |
| SA011466 | 52 | positive | Yes | F | W |
| SA011467 | 66 | positive | Yes | F | W |
| SA011468 | 12 | positive | Yes | M | B |
| SA011469 | 48 | positive | Yes | M | B |
| SA011470 | 65 | positive | Yes | M | B |
| SA011471 | 44 | positive | Yes | M | B |
| SA011472 | 39 | positive | Yes | M | B |
| SA011473 | 63 | positive | Yes | M | B |
| SA011474 | 76 | positive | Yes | M | O |
| SA011475 | 75 | positive | Yes | M | O |
| SA011476 | 80 | positive | Yes | M | O |
| SA011477 | 72 | positive | Yes | M | W |
| SA011478 | 9 | positive | Yes | M | W |
| SA011479 | 47 | positive | Yes | M | W |
| SA011480 | 46 | positive | Yes | M | W |
| SA011481 | 40 | positive | Yes | M | W |
| SA011482 | 28 | positive | Yes | M | W |
| SA011483 | 11 | positive | Yes | M | W |
| SA011484 | 79 | positive | Yes | M | W |
| SA011485 | 19 | positive | Yes | M | W |
| SA011486 | 78 | positive | Yes | M | W |
| SA011487 | 50 | positive | Yes | M | W |
| SA011488 | 49 | positive | Yes | M | W |
| SA011489 | 59 | positive | Yes | M | W |
| Showing results 1 to 100 of 100 |
Collection:
| Collection ID: | CO000261 |
| Collection Summary: | - |
| Collection Protocol Filename: | Koethe_SECIM_collection.txt |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000281 |
| Treatment Protocol Filename: | Koethe_SECIM_treatments.txt |
Sample Preparation:
| Sampleprep ID: | SP000275 |
| Sampleprep Summary: | Amino acid|Organic acid|Acylcarnitines |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf SB_OA_Assay.pdf SB_Acylcarnitine_Assay.pdf |
Combined analysis:
| Analysis ID | AN000394 | AN000395 | AN000396 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Thermo Quantiva QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | uM | uM |
Chromatography:
| Chromatography ID: | CH001335 |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001336 |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001337 |
| Methods Filename: | SB_Acylcarnitine_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000338 |
| Analysis ID: | AN000394 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | POSITIVE |
| Ion Mode: | POSITIVE |
| MS ID: | MS000339 |
| Analysis ID: | AN000395 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | POSITIVE |
| Ion Mode: | POSITIVE |
| MS ID: | MS000340 |
| Analysis ID: | AN000396 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | POSITIVE |
| Ion Mode: | POSITIVE |
