Summary of Study ST000250

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000202. The data can be accessed directly via it's Project DOI: 10.21228/M88W23 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000250
Study TitleThe Role of Obesity and Adipocytes in Immune Activation on Antiretroviral Therapy
Study TypeObservational, no intervention
Study SummaryCross-sectional, three arms
University of Florida
Last NameKoethe
First NameJohn
AddressA2200-MCN, 1161 21st Ave South, Nashville, TN 37232-2582
Submit Date2015-01-07
Num Groups3
Total Subjects100 patients, one sample per patient
Raw Data AvailableNo
Raw Data File Type(s)raw(Thermo), d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
John Koethe John Koethe application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000202
Project DOI:doi: 10.21228/M88W23
Project Title:The Role of Obesity and Adipocytes in Immune Activation on Antiretroviral Therapy
Project Type:Clinical research
Project Summary:The goal of this study is the use metabolomics to probe the role of BCAA metabolism in the apthogenisis of insulin-resistance associated with HIV-infection/ART. We will also quantify the plasma levels of 2-HBA in HIV-infected, ART -treated patients. These patients were substratified into obese or lean subjects
Institute:Vanderbilt University
Department:Division of Infectious Diseases
Last Name:Koethe
First Name:John
Address:A2200-MCN, 1161 21st Ave South, Nashville, TN 37232-2582
Funding Source:National Institute of Allergy and Infectious Diseases


Subject ID:SU000270
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human


Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id HIV status Obesity Sex Race
SA011390523negative Yes F B
SA011391524negative Yes F B
SA011392520negative Yes F B
SA011393516negative Yes F B
SA011394509negative Yes F B
SA011395515negative Yes F B
SA011396527negative Yes F B
SA011397517negative Yes F B
SA011398514negative Yes F W
SA011399513negative Yes F W
SA011400505negative Yes F W
SA011401502negative Yes F W
SA011402534negative Yes F W
SA011403533negative Yes F W
SA011404521negative Yes F W
SA011405525negative Yes F W
SA011406503negative Yes F W
SA011407501negative Yes F W
SA011408512negative Yes M B
SA011409528negative Yes M B
SA011410531negative Yes M B
SA011411507negative Yes M B
SA011412510negative Yes M W
SA011413506negative Yes M W
SA011414511negative Yes M W
SA011415508negative Yes M W
SA011416519negative Yes M W
SA011417518negative Yes M W
SA011418530negative Yes M W
SA011419532negative Yes M W
SA01142042positive No F B
SA0114213positive No F B
SA01142241positive No F B
SA01142371positive No F B
SA01142423positive No F B
SA01142558positive No F B
SA01142624positive No F B
SA01142733positive No F B
SA01142834positive No F B
SA01142937positive No F B
SA01143060positive No F W
SA01143155positive No F W
SA01143261positive No F W
SA01143343positive No F W
SA01143430positive No M B
SA01143513positive No M B
SA01143635positive No M B
SA01143714positive No M B
SA0114384positive No M B
SA01143927positive No M B
SA0114401positive No M O
SA01144167positive No M W
SA01144257positive No M W
SA01144316positive No M W
SA01144454positive No M W
SA0114458positive No M W
SA01144636positive No M W
SA0114477positive No M W
SA01144826positive No M W
SA0114496positive No M W
SA01145031positive No M W
SA0114515positive No M W
SA01145217positive Yes F B
SA01145320positive Yes F B
SA01145432positive Yes F B
SA01145525positive Yes F B
SA01145674positive Yes F B
SA01145756positive Yes F B
SA01145864positive Yes F B
SA01145969positive Yes F B
SA01146077positive Yes F B
SA01146115positive Yes F B
SA01146210positive Yes F B
SA01146362positive Yes F B
SA01146422positive Yes F W
SA01146545positive Yes F W
SA01146652positive Yes F W
SA01146766positive Yes F W
SA01146812positive Yes M B
SA01146948positive Yes M B
SA01147065positive Yes M B
SA01147144positive Yes M B
SA01147239positive Yes M B
SA01147363positive Yes M B
SA01147476positive Yes M O
SA01147575positive Yes M O
SA01147680positive Yes M O
SA01147772positive Yes M W
SA0114789positive Yes M W
SA01147947positive Yes M W
SA01148046positive Yes M W
SA01148140positive Yes M W
SA01148228positive Yes M W
SA01148311positive Yes M W
SA01148479positive Yes M W
SA01148519positive Yes M W
SA01148678positive Yes M W
SA01148750positive Yes M W
SA01148849positive Yes M W
SA01148959positive Yes M W
Showing results 1 to 100 of 100


Collection ID:CO000261
Collection Summary:-
Collection Protocol Filename:Koethe_SECIM_collection.txt
Sample Type:Blood


Treatment ID:TR000281
Treatment Protocol Filename:Koethe_SECIM_treatments.txt

Sample Preparation:

Sampleprep ID:SP000275
Sampleprep Summary:Amino acid|Organic acid|Acylcarnitines
Sampleprep Protocol Filename:SB_AA_Assay.pdf

Combined analysis:

Analysis ID AN000394 AN000395 AN000396
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Thermo Dionex Ultimate 3000 Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Thermo Quantiva QQQ Agilent 6490 QQQ
Units uM uM


Chromatography ID:CH001335
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase
Chromatography ID:CH001336
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:45
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
Chromatography ID:CH001337
Methods Filename:SB_Acylcarnitine_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Chromatography Type:Reversed phase


MS ID:MS000338
Analysis ID:AN000394
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS ID:MS000339
Analysis ID:AN000395
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS ID:MS000340
Analysis ID:AN000396
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole