Summary of Study ST000251

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000203. The data can be accessed directly via it's Project DOI: 10.21228/M8530G This work is supported by NIH grant, U2C- DK119886.


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Study IDST000251
Study TitleAmino Acid levels in preB cells under starvation
Study Typetreatment
Study Summarypre B cells from wt and EuMyc mice were grown in normal media and then placed for 2hrs in EBSS.
University of Florida
Last NameSchaub
First NameFranz
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Submit Date2015-08-15
Num Groups4
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size70 M
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Franz Schaub Franz Schaub application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000203
Project DOI:doi: 10.21228/M8530G
Project Title:Intracellular Amino Acid levels in response to MYC
Project Type:Targeted LC-MS Metabolomics
Project Summary:Hypothesis:MYC is suppressing the autophagy pathway which leads to impaired recycling of proteins and reduced free Amino Acids in the cells.
Institute:Moffitt Cancer Center
Department:Tumor Biology
Last Name:Cleveland
First Name:John
Address:12902 Magnolia Dr
Phone:813 745 3888
Funding Source:NIH


Subject ID:SU000271
Subject Type:Animal cells
Subject Species:Mus musculus
Taxonomy ID:10090
Animal Animal Supplier:inhouse breeding
Cell Biosource Or Supplier:BM derived B cells
Cell Strain Details:wt and EuMyc
Cell Primary Immortalized:primary
Species Group:Mammal


Subject type: Animal cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment media
SA011490CC268_EBSS1EuMyc transgene EBSS
SA011491CC268_EBSS2EuMyc transgene EBSS
SA011492CC268_EBSS3EuMyc transgene EBSS
SA011493CC268_N2EuMyc transgene normal media
SA011494CC268_N3EuMyc transgene normal media
SA011495CC268_N1EuMyc transgene normal media
SA011496CC267_EBSS2wt EBSS
SA011497CC267_EBSS3wt EBSS
SA011498CC267_EBSS1wt EBSS
SA011499CC267_N2wt normal media
SA011500CC267_N3wt normal media
SA011501CC267_N1wt normal media
Showing results 1 to 12 of 12


Collection ID:CO000262
Collection Summary:Cells were counted, washed with cold PBS and then flash-frozen in liquid N2
Sample Type:cells
Volumeoramount Collected:0.5.00E+07 cells
Storage Vials:screw caps


Treatment ID:TR000282
Treatment Summary:Cells were incubated for 2hrs in EBSS at 37C.
Cell Media:45%DMEM, 45%IMDM, 10%FCS, Q, PS, Sodium Pyruvate, beta mercaptoethanol and EBSS with 10% dialized FBS
Cell Harvesting:washed, and pellet flashfroozen in liquid N2
Cell Pct Confluence:2xE06 per ml

Sample Preparation:

Sampleprep ID:SP000276
Sampleprep Summary:Amino acid

Combined analysis:

Analysis ID AN000397
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um)
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Units pmol/million cells


Chromatography ID:CH000281
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase


MS ID:MS000341
Analysis ID:AN000397
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole