Summary of Study ST000252

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000204. The data can be accessed directly via it's Project DOI: 10.21228/M81C7N This work is supported by NIH grant, U2C- DK119886.

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Study IDST000252
Study TitleImpact of recurrent hypoglycemia on brain metabolite profile
Study TypeInsulin treatment in diabetic rats
Study SummaryThe goal of this study is to determine the effect of mild / moderate hypoglycemia on brain metabolism. To achieve this goal insulin-treated diabetic rats will be subjected to recurrent mild / moderate hypoglycemia.
Institute
University of Florida
DepartmentSECIM
Last NameDave
First NameKunjan
EmailKdave@med.miami.edu
Phone305-243-3590
Submit Date2015-08-06
Num Groups2
Total Subjects40
Study CommentsTwo groups (i) Insulin treated diabetic + Recurrent hypoglycemia, (ii) Insulin treated diabetic + Recurrent hypoglycemia (only insulin injection) + Glucose infusion to maintain glucose to baseline levels.Total 40 samples. Description: Ten hippocampus samples and 10 hypothalamus sample from ITD+RH group of rats.Similarly, Ten hippocampus samples and 10 hypothalamus sample from ITD+RH+Glucose group of rats.
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size4.8 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Kunjan Dave Kunjan Dave
https://dx.doi.org/10.21228/M81C7N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000204
Project DOI:doi: 10.21228/M81C7N
Project Title:Impact of recurrent hypoglycemia on brain metabolite profile.
Institute:University of Florida
Department:SECIM
Last Name:Dave
First Name:Kunjan
Address:1420 N.W 9th Avenue, Two Story Lab, Room no 203, Locator code : D4-5
Email:Kdave@med.miami.edu
Funding Source:Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209

Subject:

Subject ID:SU000272
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Wistar
Gender:Male
Animal Animal Supplier:Charles River Laboratories
Animal Housing:Division of Veterenary Resources, University of Miami
Animal Light Cycle:12 hour day / light cycle
Animal Feed:Ad libitum
Animal Water:Ad libitum
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment sample type
SA011502KD_386927ITD+RH+Glucose Hippocampus
SA011503KD_386926ITD+RH+Glucose Hippocampus
SA011504KD_386165ITD+RH+Glucose Hippocampus
SA011505KD_382783ITD+RH+Glucose Hippocampus
SA011506KD_388421ITD+RH+Glucose Hippocampus
SA011507KD_386937ITD+RH+Glucose Hippocampus
SA011508KD_388433ITD+RH+Glucose Hippocampus
SA011509KD_388430ITD+RH+Glucose Hippocampus
SA011510KD_388432ITD+RH+Glucose Hippocampus
SA011511KD_388426ITD+RH+Glucose Hippocampus
SA011512KD_386927_2ITD+RH+Glucose Hypothalamus
SA011513KD_386926_2ITD+RH+Glucose Hypothalamus
SA011514KD_386165_2ITD+RH+Glucose Hypothalamus
SA011515KD_386937_2ITD+RH+Glucose Hypothalamus
SA011516KD_388421_2ITD+RH+Glucose Hypothalamus
SA011517KD_388433_2ITD+RH+Glucose Hypothalamus
SA011518KD_382783_2ITD+RH+Glucose Hypothalamus
SA011519KD_388432_2ITD+RH+Glucose Hypothalamus
SA011520KD_388426_2ITD+RH+Glucose Hypothalamus
SA011521KD_388430_2ITD+RH+Glucose Hypothalamus
SA011522KD_385112ITD+RH Hippocampus
SA011523KD_385113ITD+RH Hippocampus
SA011524KD_382772ITD+RH Hippocampus
SA011525KD_386171ITD+RH Hippocampus
SA011526KD_385115ITD+RH Hippocampus
SA011527KD_388427ITD+RH Hippocampus
SA011528KD_382781ITD+RH Hippocampus
SA011529KD_388422ITD+RH Hippocampus
SA011530KD_388420ITD+RH Hippocampus
SA011531KD_386935ITD+RH Hippocampus
SA011532KD_388422_2ITD+RH Hypothalamus
SA011533KD_385115_2ITD+RH Hypothalamus
SA011534KD_382772_2ITD+RH Hypothalamus
SA011535KD_382781_2ITD+RH Hypothalamus
SA011536KD_385112_2ITD+RH Hypothalamus
SA011537KD_385113_2ITD+RH Hypothalamus
SA011538KD_388420_2ITD+RH Hypothalamus
SA011539KD_386935_2ITD+RH Hypothalamus
SA011540KD_386171_2ITD+RH Hypothalamus
SA011541KD_388427_2ITD+RH Hypothalamus
Showing results 1 to 40 of 40

Collection:

Collection ID:CO000263
Collection Summary:Rats were euthanized under isoflurane anesthesia (30% O2 / 70% N2O)
Sample Type:Tissue
Collection Method:Hippocampus and Hypothalamus were disected out qucikly and were frozen in liquid nitrogen.
Volumeoramount Collected:Entire hippocampus and hypothalamus were collected.
Storage Conditions:Samples were stored at - 80 o C freezer and shipped to Metabolomics facility on dry ice.
Collection Vials:1.5 ml tubes
Storage Vials:1.5 ml tubes
Collection Tube Temp:RT at the time of sample collection
Tissue Cell Identification:Hippocampus and hypothalamus
Tissue Cell Quantity Taken:Entire hippocampus and hypothalamus were collected.

Treatment:

Treatment ID:TR000283
Treatment Summary:Two groups (i) Insulin-treated diabetic + Recurrent hypoglycemia, (ii) Insulin-treated diabetic + Recurrent hypoglycemia (only insulin injection without hypoglycemia) + Glucose infusion to maintain glucose to baseline levels.

Sample Preparation:

Sampleprep ID:SP000277
Sampleprep Summary:-
Sampleprep Protocol Filename:Brain_Tissue_Sampleprep.pdf
Sampleprep Protocol Comments:Appendix C_Protein_DNA_quant.pdf
Organ:Brain

Combined analysis:

Analysis ID AN000398 AN000399
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak Area

Chromatography:

Chromatography ID:CH000282
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000342
Analysis ID:AN000398
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000343
Analysis ID:AN000399
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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