Summary of Study ST000252

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000204. The data can be accessed directly via it's Project DOI: 10.21228/M81C7N This work is supported by NIH grant, U2C- DK119886.


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Study IDST000252
Study TitleImpact of recurrent hypoglycemia on brain metabolite profile
Study TypeInsulin treatment in diabetic rats
Study SummaryThe goal of this study is to determine the effect of mild / moderate hypoglycemia on brain metabolism. To achieve this goal insulin-treated diabetic rats will be subjected to recurrent mild / moderate hypoglycemia.
University of Florida
Last NameDave
First NameKunjan
Submit Date2015-08-06
Num Groups2
Total Subjects40
Study CommentsTwo groups (i) Insulin treated diabetic + Recurrent hypoglycemia, (ii) Insulin treated diabetic + Recurrent hypoglycemia (only insulin injection) + Glucose infusion to maintain glucose to baseline levels.Total 40 samples. Description: Ten hippocampus samples and 10 hypothalamus sample from ITD+RH group of rats.Similarly, Ten hippocampus samples and 10 hypothalamus sample from ITD+RH+Glucose group of rats.
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size4.8 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Kunjan Dave Kunjan Dave application/zip

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Project ID:PR000204
Project DOI:doi: 10.21228/M81C7N
Project Title:Impact of recurrent hypoglycemia on brain metabolite profile.
Institute:University of Florida
Last Name:Dave
First Name:Kunjan
Address:1420 N.W 9th Avenue, Two Story Lab, Room no 203, Locator code : D4-5
Funding Source:Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209


Subject ID:SU000272
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Wistar
Animal Animal Supplier:Charles River Laboratories
Animal Housing:Division of Veterenary Resources, University of Miami
Animal Light Cycle:12 hour day / light cycle
Animal Feed:Ad libitum
Animal Water:Ad libitum
Species Group:Mammal


Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment sample type
SA011502KD_386927ITD+RH+Glucose Hippocampus
SA011503KD_386926ITD+RH+Glucose Hippocampus
SA011504KD_386165ITD+RH+Glucose Hippocampus
SA011505KD_382783ITD+RH+Glucose Hippocampus
SA011506KD_388421ITD+RH+Glucose Hippocampus
SA011507KD_386937ITD+RH+Glucose Hippocampus
SA011508KD_388433ITD+RH+Glucose Hippocampus
SA011509KD_388430ITD+RH+Glucose Hippocampus
SA011510KD_388432ITD+RH+Glucose Hippocampus
SA011511KD_388426ITD+RH+Glucose Hippocampus
SA011512KD_386927_2ITD+RH+Glucose Hypothalamus
SA011513KD_386926_2ITD+RH+Glucose Hypothalamus
SA011514KD_386165_2ITD+RH+Glucose Hypothalamus
SA011515KD_386937_2ITD+RH+Glucose Hypothalamus
SA011516KD_388421_2ITD+RH+Glucose Hypothalamus
SA011517KD_388433_2ITD+RH+Glucose Hypothalamus
SA011518KD_382783_2ITD+RH+Glucose Hypothalamus
SA011519KD_388432_2ITD+RH+Glucose Hypothalamus
SA011520KD_388426_2ITD+RH+Glucose Hypothalamus
SA011521KD_388430_2ITD+RH+Glucose Hypothalamus
SA011522KD_385112ITD+RH Hippocampus
SA011523KD_385113ITD+RH Hippocampus
SA011524KD_382772ITD+RH Hippocampus
SA011525KD_386171ITD+RH Hippocampus
SA011526KD_385115ITD+RH Hippocampus
SA011527KD_388427ITD+RH Hippocampus
SA011528KD_382781ITD+RH Hippocampus
SA011529KD_388422ITD+RH Hippocampus
SA011530KD_388420ITD+RH Hippocampus
SA011531KD_386935ITD+RH Hippocampus
SA011532KD_388422_2ITD+RH Hypothalamus
SA011533KD_385115_2ITD+RH Hypothalamus
SA011534KD_382772_2ITD+RH Hypothalamus
SA011535KD_382781_2ITD+RH Hypothalamus
SA011536KD_385112_2ITD+RH Hypothalamus
SA011537KD_385113_2ITD+RH Hypothalamus
SA011538KD_388420_2ITD+RH Hypothalamus
SA011539KD_386935_2ITD+RH Hypothalamus
SA011540KD_386171_2ITD+RH Hypothalamus
SA011541KD_388427_2ITD+RH Hypothalamus
Showing results 1 to 40 of 40


Collection ID:CO000263
Collection Summary:Rats were euthanized under isoflurane anesthesia (30% O2 / 70% N2O)
Sample Type:Tissue
Collection Method:Hippocampus and Hypothalamus were disected out qucikly and were frozen in liquid nitrogen.
Volumeoramount Collected:Entire hippocampus and hypothalamus were collected.
Storage Conditions:Samples were stored at - 80 o C freezer and shipped to Metabolomics facility on dry ice.
Collection Vials:1.5 ml tubes
Storage Vials:1.5 ml tubes
Collection Tube Temp:RT at the time of sample collection
Tissue Cell Identification:Hippocampus and hypothalamus
Tissue Cell Quantity Taken:Entire hippocampus and hypothalamus were collected.


Treatment ID:TR000283
Treatment Summary:Two groups (i) Insulin-treated diabetic + Recurrent hypoglycemia, (ii) Insulin-treated diabetic + Recurrent hypoglycemia (only insulin injection without hypoglycemia) + Glucose infusion to maintain glucose to baseline levels.

Sample Preparation:

Sampleprep ID:SP000277
Sampleprep Summary:-
Sampleprep Protocol Filename:Brain_Tissue_Sampleprep.pdf
Sampleprep Protocol Comments:Appendix C_Protein_DNA_quant.pdf

Combined analysis:

Analysis ID AN000398 AN000399
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units Peak area Peak Area


Chromatography ID:CH000282
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase


MS ID:MS000342
Analysis ID:AN000398
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS ID:MS000343
Analysis ID:AN000399
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap