Summary of Study ST000252
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000204. The data can be accessed directly via it's Project DOI: 10.21228/M81C7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000252 |
Study Title | Impact of recurrent hypoglycemia on brain metabolite profile |
Study Type | Insulin treatment in diabetic rats |
Study Summary | The goal of this study is to determine the effect of mild / moderate hypoglycemia on brain metabolism. To achieve this goal insulin-treated diabetic rats will be subjected to recurrent mild / moderate hypoglycemia. |
Institute | University of Florida |
Department | SECIM |
Last Name | Dave |
First Name | Kunjan |
Kdave@med.miami.edu | |
Phone | 305-243-3590 |
Submit Date | 2015-08-06 |
Num Groups | 2 |
Total Subjects | 40 |
Study Comments | Two groups (i) Insulin treated diabetic + Recurrent hypoglycemia, (ii) Insulin treated diabetic + Recurrent hypoglycemia (only insulin injection) + Glucose infusion to maintain glucose to baseline levels.Total 40 samples. Description: Ten hippocampus samples and 10 hypothalamus sample from ITD+RH group of rats.Similarly, Ten hippocampus samples and 10 hypothalamus sample from ITD+RH+Glucose group of rats. |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 4.8 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000204 |
Project DOI: | doi: 10.21228/M81C7N |
Project Title: | Impact of recurrent hypoglycemia on brain metabolite profile. |
Institute: | University of Florida |
Department: | SECIM |
Last Name: | Dave |
First Name: | Kunjan |
Address: | 1420 N.W 9th Avenue, Two Story Lab, Room no 203, Locator code : D4-5 |
Email: | Kdave@med.miami.edu |
Funding Source: | Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 |
Subject:
Subject ID: | SU000272 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Wistar |
Gender: | Male |
Animal Animal Supplier: | Charles River Laboratories |
Animal Housing: | Division of Veterenary Resources, University of Miami |
Animal Light Cycle: | 12 hour day / light cycle |
Animal Feed: | Ad libitum |
Animal Water: | Ad libitum |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | sample type |
---|---|---|---|
SA011502 | KD_386927 | ITD+RH+Glucose | Hippocampus |
SA011503 | KD_386926 | ITD+RH+Glucose | Hippocampus |
SA011504 | KD_386165 | ITD+RH+Glucose | Hippocampus |
SA011505 | KD_382783 | ITD+RH+Glucose | Hippocampus |
SA011506 | KD_388421 | ITD+RH+Glucose | Hippocampus |
SA011507 | KD_386937 | ITD+RH+Glucose | Hippocampus |
SA011508 | KD_388433 | ITD+RH+Glucose | Hippocampus |
SA011509 | KD_388430 | ITD+RH+Glucose | Hippocampus |
SA011510 | KD_388432 | ITD+RH+Glucose | Hippocampus |
SA011511 | KD_388426 | ITD+RH+Glucose | Hippocampus |
SA011512 | KD_386927_2 | ITD+RH+Glucose | Hypothalamus |
SA011513 | KD_386926_2 | ITD+RH+Glucose | Hypothalamus |
SA011514 | KD_386165_2 | ITD+RH+Glucose | Hypothalamus |
SA011515 | KD_386937_2 | ITD+RH+Glucose | Hypothalamus |
SA011516 | KD_388421_2 | ITD+RH+Glucose | Hypothalamus |
SA011517 | KD_388433_2 | ITD+RH+Glucose | Hypothalamus |
SA011518 | KD_382783_2 | ITD+RH+Glucose | Hypothalamus |
SA011519 | KD_388432_2 | ITD+RH+Glucose | Hypothalamus |
SA011520 | KD_388426_2 | ITD+RH+Glucose | Hypothalamus |
SA011521 | KD_388430_2 | ITD+RH+Glucose | Hypothalamus |
SA011522 | KD_385112 | ITD+RH | Hippocampus |
SA011523 | KD_385113 | ITD+RH | Hippocampus |
SA011524 | KD_382772 | ITD+RH | Hippocampus |
SA011525 | KD_386171 | ITD+RH | Hippocampus |
SA011526 | KD_385115 | ITD+RH | Hippocampus |
SA011527 | KD_388427 | ITD+RH | Hippocampus |
SA011528 | KD_382781 | ITD+RH | Hippocampus |
SA011529 | KD_388422 | ITD+RH | Hippocampus |
SA011530 | KD_388420 | ITD+RH | Hippocampus |
SA011531 | KD_386935 | ITD+RH | Hippocampus |
SA011532 | KD_388422_2 | ITD+RH | Hypothalamus |
SA011533 | KD_385115_2 | ITD+RH | Hypothalamus |
SA011534 | KD_382772_2 | ITD+RH | Hypothalamus |
SA011535 | KD_382781_2 | ITD+RH | Hypothalamus |
SA011536 | KD_385112_2 | ITD+RH | Hypothalamus |
SA011537 | KD_385113_2 | ITD+RH | Hypothalamus |
SA011538 | KD_388420_2 | ITD+RH | Hypothalamus |
SA011539 | KD_386935_2 | ITD+RH | Hypothalamus |
SA011540 | KD_386171_2 | ITD+RH | Hypothalamus |
SA011541 | KD_388427_2 | ITD+RH | Hypothalamus |
Showing results 1 to 40 of 40 |
Collection:
Collection ID: | CO000263 |
Collection Summary: | Rats were euthanized under isoflurane anesthesia (30% O2 / 70% N2O) |
Sample Type: | Tissue |
Collection Method: | Hippocampus and Hypothalamus were disected out qucikly and were frozen in liquid nitrogen. |
Volumeoramount Collected: | Entire hippocampus and hypothalamus were collected. |
Storage Conditions: | Samples were stored at - 80 o C freezer and shipped to Metabolomics facility on dry ice. |
Collection Vials: | 1.5 ml tubes |
Storage Vials: | 1.5 ml tubes |
Collection Tube Temp: | RT at the time of sample collection |
Tissue Cell Identification: | Hippocampus and hypothalamus |
Tissue Cell Quantity Taken: | Entire hippocampus and hypothalamus were collected. |
Treatment:
Treatment ID: | TR000283 |
Treatment Summary: | Two groups (i) Insulin-treated diabetic + Recurrent hypoglycemia, (ii) Insulin-treated diabetic + Recurrent hypoglycemia (only insulin injection without hypoglycemia) + Glucose infusion to maintain glucose to baseline levels. |
Sample Preparation:
Sampleprep ID: | SP000277 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Brain_Tissue_Sampleprep.pdf |
Sampleprep Protocol Comments: | Appendix C_Protein_DNA_quant.pdf |
Organ: | Brain |
Combined analysis:
Analysis ID | AN000398 | AN000399 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak Area |
Chromatography:
Chromatography ID: | CH000282 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000342 |
Analysis ID: | AN000398 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000343 |
Analysis ID: | AN000399 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |