Summary of Study ST000270

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000214. The data can be accessed directly via it's Project DOI: 10.21228/M8QW24 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000270
Study TitleMetabolomics in AML
Study TypeSerum samples from Mla patients responsive or not responsive to chemotherapy
Study SummaryIn the current study we will perform global metabolic profiling on serum samples obtained at diagnosis from pediatric AML patients (n=20) treated under St. Jude AML02 clinical trial to identify potential biomarkers of clinical significance. These patients include 10 responders and 10 non responders. In a subset of patients (n=7), we have matched samples that were obtained at remission allowing us to determine the change in serum metabolome at diagnosis and after remission followed by investigation of the metabolome change analysis with clinical response.
Institute
University of Florida
DepartmentSECIM
LaboratoryPharmacotherpy and Translational Research
Last NameLamba
First NameJatinder
Emailjlamba@ufl.edu
Phone3522736425
Submit Date2015-08-27
Total Subjects27
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Jatinder Lamba Jatinder Lamba
https://dx.doi.org/10.21228/M8QW24
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000214
Project DOI:doi: 10.21228/M8QW24
Project Title:Metabolomics in AML
Project Summary:In the current study we will perform global metabolic profiling on serum samples obtained at diagnosis from pediatric AML patients
Institute:University of Florida
Department:Pharmacotherapy and Translational Research
Last Name:Lamba
First Name:Jatinder
Email:jlamba@ufl.edu
Phone:3522736425

Subject:

Subject ID:SU000290
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:9606
Weight Or Weight Range:0-21
Human Exclusion Criteria:Pediatric Patients with AML (Age<21 years)
Animal Animal Supplier:Aml patients >21 years of age
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA012113Lamba_TB-05-2769Diagnosis -No treatment
SA012114Lamba_TB-02-2506Diagnosis -No treatment
SA012115Lamba_TB-05-2681Diagnosis -No treatment
SA012116Lamba_TB-05-2581Diagnosis -No treatment
SA012117Lamba_TB-06-2927Diagnosis -No treatment
SA012118Lamba_TB-07-0918Diagnosis -No treatment
SA012119Lamba_TB-05-0552Diagnosis -No treatment
SA012120Lamba_TB-07-1889Diagnosis -No treatment
SA012121Lamba_TB-07-1864Diagnosis -No treatment
SA012122Lamba_TB-05-2457Diagnosis -No treatment
SA012123Lamba_TB-05-2702Diagnosis -No treatment
SA012124Lamba_TB-03-2137Diagnosis -No treatment
SA012125Lamba_TB-05-0422Diagnosis -No treatment
SA012126Lamba_TB-03-0147Diagnosis -No treatment
SA012127Lamba_TB-03-2257Diagnosis -No treatment
SA012128Lamba_TB-03-0684Diagnosis -No treatment
SA012129Lamba_TB-04-1048Diagnosis -No treatment
SA012130Lamba_TB-04-1495Diagnosis -No treatment
SA012131Lamba_TB-04-1584Diagnosis -No treatment
SA012132Lamba_TB-04-1340Diagnosis -No treatment
SA012133Lamba_TB-13-3871Post remission
SA012134Lamba_TB-12-0873Post remission
SA012135Lamba_TB-12-3284Post remission
SA012136Lamba_TB-10-0259Post remission
SA012137Lamba_TB-09-0224Post remission
SA012138Lamba_TB-09-2654Post remission
SA012139Lamba_TB-10-1229Post remission
Showing results 1 to 27 of 27

Collection:

Collection ID:CO000284
Collection Summary:Blood samples were collected at diagnosis and after patients achieved remission after chemotherapy
Sample Type:Blood collected and serum separated
Collection Location:Memphis
Storage Conditions:-80 C
Blood Serum Or Plasma:Blood Serum

Treatment:

Treatment ID:TR000304
Treatment Summary:Most of the samples are at diagnosis without any treatment, 7 samples were received after pateints had treatment with cytarabine, Daunorubicin, and etoposide

Sample Preparation:

Sampleprep ID:SP000298
Sampleprep Summary:-
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000432 AN000433
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000302
Methods Filename:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000373
Analysis ID:AN000432
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000374
Analysis ID:AN000433
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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