Summary of Study ST000271
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000215. The data can be accessed directly via it's Project DOI: 10.21228/M8M59M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000271 |
| Study Title | C13 Pyruvate Flux in 3t3 L1 |
| Study Type | 13C mass isotopomer analysis (flux studies) |
| Study Summary | Pyruvate isotopically labeled by 13C either at position 1 or 2 was used to investigate carbon flux in 3T3-L1 mouse cells line in the presence of various combinations of drugs |
| Institute | University of Michigan |
| Department | Molecular and Integrative Physiology (MCTP) |
| Laboratory | Macdougald Lab (MCTP) |
| Last Name | MacDougald |
| First Name | Ormond |
| Address | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| macdouga@umich.edu | |
| Phone | 734-647-4880 |
| Submit Date | 2014-01-21 |
| Num Groups | 10 |
| Total Subjects | 10 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000215 |
| Project DOI: | doi: 10.21228/M8M59M |
| Project Title: | 13C mass isotopomer analysis (flux studies) |
| Project Summary: | 13 Flux in lipid metabolism |
| Institute: | University of Michigan |
| Department: | Molecular and Integrative Physiology (MCTP) |
| Laboratory: | Macdougald Lab |
| Last Name: | MacDougald |
| First Name: | Ormond |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | macdouga@umich.edu |
| Phone: | 734-647-4880 |
Subject:
| Subject ID: | SU000291 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Drug combination | 13C-label position in pyruvate |
|---|---|---|---|
| SA012142 | S00012694 | Isoproterenol | C1 |
| SA012143 | S00012695 | Isoproterenol | C2 |
| SA012140 | S00012692 | Isoproterenol + Leelamine | C1 |
| SA012141 | S00012693 | Isoproterenol + Leelamine | C2 |
| SA012144 | S00012688 | None | C1 |
| SA012145 | S00012689 | None | C2 |
| SA012148 | S00012696 | Rosiglitazone | C1 |
| SA012149 | S00012697 | Rosiglitazone | C2 |
| SA012146 | S00012690 | Rosiglitazone + Leelamine | C1 |
| SA012147 | S00012691 | Rosiglitazone + Leelamine | C2 |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000285 |
| Collection Summary: | - |
| Sample Type: | Cell Line |
Treatment:
| Treatment ID: | TR000305 |
Sample Preparation:
| Sampleprep ID: | SP000299 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000434 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000303 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000375 |
| Analysis ID: | AN000434 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| Processing Parameters File: | EX00242-flux-MassHunterQuant-GlyTCA-DataAnalysis-Method.m.zip |
