Summary of Study ST000274
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000218. The data can be accessed directly via it's Project DOI: 10.21228/M86W2G This work is supported by NIH grant, U2C- DK119886.
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| Study ID | ST000274 |
| Study Title | HIF 1 alpha type 2 cells metabolomics |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | Lung contusion is a major risk factor for the development of acute respiratory distress syndrome. Hypoxia-inducible factor-1? is the primary transcription factor that is responsible for regulating the cellular response to changes in oxygen tension. We set to determine if hypoxia-inducible factor-1? plays a role in the pathogenesis of acute inflammatory response and injury in lung contusion. Nonlethal closed-chest unilateral lung contusion was induced in a hypoxia reporter mouse model and type 2 cell-specific hypoxia-inducible factor-1? conditional knockout mice. The mice were killed at 5-, 24-, 48-, and 72-hour time points, and the extent of systemic and tissue hypoxia was assessed. In addition, injury and inflammation were assessed by measuring bronchoalveolar lavage cells (flow cytometry and cytospin), albumin (permeability injury), and cytokines (inflammation). Isolated type 2 cells from the hypoxia-inducible factor-1? conditional knockout mice were isolated and evaluated for proinflammatory cytokines following lung contusion. Finally, the role of nuclear factor-?B and interleukin-1? as intermediates in this interaction was studied. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/ |
| Institute | University of Michigan |
| Department | Surgery |
| Laboratory | Raghavendran Lab |
| Last Name | Thomas |
| First Name | Bivin |
| Address | Ann Arbor, MI |
| bthomas@umich.edu | |
| Phone | 734-615-7142 |
| Submit Date | 2014-06-04 |
| Num Groups | 4 |
| Total Subjects | 8 |
| Study Comments | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/ : HIF1 (+/+) stands for hypoxia reporter mouse model HIF1 (-/-) stands for type 2 cell-specific hypoxia-inducible factor-1? conditional knockout |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000218 |
| Project DOI: | doi: 10.21228/M86W2G |
| Project Title: | HIF 1 alpha |
| Project Summary: | Glycolysis/TCA/Nucleotide analysis (AEC cells) |
| Institute: | University of Michigan |
| Department: | Surgery |
| Laboratory: | Raghavendran Lab |
| Last Name: | Raghavendran |
| First Name: | Krishnan |
| Address: | Ann Arbor, MI |
| Email: | kraghave@umich.edu |
| Phone: | 734-615-7142 |
Subject:
| Subject ID: | SU000294 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Treatment |
|---|---|---|---|
| SA012205 | S00014944 | HIF1 (+/+) | Control |
| SA012206 | S00014945 | HIF1 (+/+) | Control |
| SA012209 | S00014949 | HIF1 (-/-) | Control |
| SA012210 | S00014948 | HIF1 (-/-) | Control |
| SA012207 | S00014947 | HIF1 (+/+) | Lung contusion, 24h |
| SA012208 | S00014946 | HIF1 (+/+) | Lung contusion, 24h |
| SA012211 | S00014950 | HIF1 (-/-) | Lung contusion, 24h |
| SA012212 | S00014951 | HIF1 (-/-) | Lung contusion, 24h |
| SA012213 | S00020491 | Pooled sample | Pooled cells |
| Showing results 1 to 9 of 9 |
Collection:
| Collection ID: | CO000288 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000308 |
Sample Preparation:
| Sampleprep ID: | SP000302 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000437 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE |
| Units | pmol/µg protein |
Chromatography:
| Chromatography ID: | CH000306 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000378 |
| Analysis ID: | AN000437 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| Processing Parameters File: | EX00307-MassHunterQuant-GlyTCA-LCMS-DataAnalysis-Method.m |
