Summary of Study ST000278
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000222. The data can be accessed directly via it's Project DOI: 10.21228/M8TP4R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000278 |
| Study Title | Viral Effect on Metabolism (part I) |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | Small airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer. |
| Institute | University of Michigan |
| Department | Department of Pediatrics |
| Laboratory | Casola Lab (University of Texas Medical Branch) |
| Last Name | Zhao |
| First Name | Yanhua |
| Address | Galveston, TX |
| yazhao1@utmb.edu | |
| Phone | 409-443-1461 |
| Submit Date | 2014-10-24 |
| Num Groups | 4 |
| Total Subjects | 12 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2016-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000222 |
| Project DOI: | doi: 10.21228/M8TP4R |
| Project Title: | Viral Effect on Metabolism |
| Project Type: | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Project Summary: | Global Metabolic profiling of infection by respiratory virus |
| Institute: | University of Texas |
| Department: | Department of Pediatrics |
| Laboratory: | Casola Lab |
| Last Name: | Casola |
| First Name: | Antonella |
| Address: | Galveston, TX |
| Email: | yazhao1@utmb.edu |
| Phone: | 409-772-2798 |
Subject:
| Subject ID: | SU000298 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | INFECTION TIME (0-24 Hrs) |
|---|---|---|
| SA012243 | S00016664 | 0 |
| SA012244 | S00016666 | 0 |
| SA012245 | S00016665 | 0 |
| SA012249 | S00016671 | 15 |
| SA012250 | S00016672 | 15 |
| SA012251 | S00016670 | 15 |
| SA012252 | S00016675 | 24 |
| SA012253 | S00016673 | 24 |
| SA012254 | S00016674 | 24 |
| SA012246 | S00016668 | 6 |
| SA012247 | S00016669 | 6 |
| SA012248 | S00016667 | 6 |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000292 |
| Collection Summary: | - |
| Sample Type: | Cell Line |
Treatment:
| Treatment ID: | TR000312 |
Sample Preparation:
| Sampleprep ID: | SP000306 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000443 | AN000444 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6530 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | pmol/µg protein | pmol/µg protein |
Chromatography:
| Chromatography ID: | CH000314 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001308 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000384 |
| Analysis ID: | AN000443 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| MS ID: | MS000385 |
| Analysis ID: | AN000444 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M |
| Processing Parameters File: | EX00368-MassHunterQuant-GlyTCA-GC-DataAnalysis-Method.m |
