Summary of Study ST000282
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000224. The data can be accessed directly via it's Project DOI: 10.21228/M8K599 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000282 |
| Study Title | Pilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs) |
| Study Type | 13C mass isotopomer analysis (flux studies) |
| Study Summary | Rho-GTPases are small GTP-binding proteins that contribute to the epithelial-to-mesenchymal transition by regulating several cellular processes including organization of the actin cytoskeleton, cell motility, transcription, and cell proliferation. Overexpression of RhoC-GTPases (RhoC) in breast cancer has been implicated in poor disease prognosis due to increased cancer cells invasion, migration, and motility, which warranted its consideration as a therapeutic target for inhibiting breast cancer metastasis. Using silencing RNA (siRNA) molecules to knockdown RhoC expression is a promising approach to inhibit breast cancer metastases. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Laboratory | Merajver Lab |
| Last Name | Wynn |
| First Name | Michelle |
| Address | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| mlwynn@umich.edu | |
| Phone | 734-647-3408 |
| Submit Date | 2015-02-11 |
| Num Groups | 5 |
| Total Subjects | 16 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2016-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000224 |
| Project DOI: | doi: 10.21228/M8K599 |
| Project Title: | 13C mass isotopomer analysis |
| Project Summary: | Pilot studies before designing future experiments |
| Institute: | University of Michigan |
| Department: | Internal Medicine |
| Laboratory: | Merajver Lab |
| Last Name: | Merajver |
| First Name: | Sophia |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | smerajve@med.umich.edu |
| Phone: | (734)936-6884 |
Subject:
| Subject ID: | SU000302 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell line | 13C Label | RhoC overexpression | RhoA knockdown | RhoC knockdown | RhoC drug |
|---|---|---|---|---|---|---|---|
| SA012306 | S00017282 | MCF10A | Glutamine | no | no | no | no |
| SA012307 | S00017283 | MCF10A | Glutamine | yes | no | no | no |
| SA012308 | S00017284 | MDA-MB-231 | Glutamine | no | no | no | no |
| SA012309 | S00017287 | MDA-MB-231 | Glutamine | no | no | no | yes |
| SA012310 | S00017286 | MDA-MB-231 | Glutamine | no | no | yes | no |
| SA012311 | S00017285 | MDA-MB-231 | Glutamine | no | yes | no | no |
| SA012312 | S00017295 | SUM149 | Glucose | no | no | no | no |
| SA012313 | S00017293 | SUM149 | Glucose | no | no | no | no |
| SA012314 | S00017294 | SUM149 | Glucose | no | no | no | yes |
| SA012315 | S00017290 | SUM149 | Glutamine | no | no | no | no |
| SA012316 | S00017288 | SUM149 | Glutamine | no | no | no | no |
| SA012317 | S00017289 | SUM149 | Glutamine | no | no | no | yes |
| SA012318 | S00017296 | SUM190 | Glucose | no | no | no | no |
| SA012319 | S00017297 | SUM190 | Glucose | no | no | yes | no |
| SA012320 | S00017291 | SUM190 | Glutamine | no | no | no | no |
| SA012321 | S00017292 | SUM190 | Glutamine | no | no | yes | no |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000296 |
| Collection Summary: | - |
| Sample Type: | Cell Line |
Treatment:
| Treatment ID: | TR000316 |
Sample Preparation:
| Sampleprep ID: | SP000310 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000449 | AN000450 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6530 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Area | Area normalized |
Chromatography:
| Chromatography ID: | CH000320 |
| Methods ID: | AQM010 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001310 |
| Methods ID: | AQM012 |
| Methods Filename: | MAHMOUD-PALM_14ISOTOPE.M |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000390 |
| Analysis ID: | AN000449 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
| Processing Parameters File: | EX00403-flux-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip |
| MS ID: | MS000391 |
| Analysis ID: | AN000450 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | MAHMOUD-PALM_14ISOTOPE.M.zip |
