Summary of Study ST000288

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000230. The data can be accessed directly via it's Project DOI: 10.21228/M8XG6C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000288
Study TitleMetabolomic profiling of AML Cell Lines
Study TypeIn vitro study
Study SummaryIn vitro study with AML cell lines that are treated with different concentrations of cytarabine (nucleoside analog). 8 AML cell lines were incubated for 24hr with 0uM, 1uM and 10uM ara-C. After 24hr the cells were washed and pellets were stored in -80°C for genomic and metabolomic analysis.
Institute
University of Florida
DepartmentSECIM
Last NameBhise
First NameNeha
Address-
Emailnbhise@cop.ufl.edu
Phone352-294-5745
Submit Date2015-02-15
Num Groups3
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Neha Bhise Neha Bhise
https://dx.doi.org/10.21228/M8XG6C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000230
Project DOI:doi: 10.21228/M8XG6C
Project Title:Identification of Factors Influencing Nucleoside Analog Sensitivity
Project Summary:The metabolomic profiles of cytogenetically different AML cell lines are associated with variability in response of these cell lines to nucleoside analogs. The treatment with nucleoside analog (cytarabine) would result in changes in the metabolomic profile of the AML cell lines.
Institute:University of Florida
Department:Pharmacotherapy and Translational Research
Last Name:Lamba
First Name:Jatinder
Address:-
Email:jlamba@cop.ufl.edu
Phone:352-273-6425

Subject:

Subject ID:SU000308
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:Lamba_Subjects_20150210.docx
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cytarabin Treatment (uM)
SA013134Lamba_HL60_10
SA013135Lamba_KG1_10
SA013136Lamba_ME1_10
SA013137Lamba_THP1_10
SA013138Lamba_MOLM16_10
SA013139Lamba_AML193_10
SA013140Lamba_MV-411_10
SA013141Lamba_Kasumi1_10
SA013142Lamba_KG1_21
SA013143Lamba_MOLM16_21
SA013144Lamba_ME1_21
SA013145Lamba_HL60_21
SA013146Lamba_Kasumi1_21
SA013147Lamba_MV-411_21
SA013148Lamba_THP1_21
SA013149Lamba_AML193_21
SA013150Lamba_ME1_310
SA013151Lamba_Kasumi1_310
SA013152Lamba_HL60_310
SA013153Lamba_THP1_310
SA013154Lamba_KG1_310
SA013155Lamba_MV-411_310
SA013156Lamba_AML193_310
SA013157Lamba_MOLM16_310
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000302
Collection Summary:See protocol file
Collection Protocol Filename:Lamba_Collection_20150210.docx
Sample Type:Cells

Treatment:

Treatment ID:TR000322
Treatment Summary:See treatment file
Treatment Protocol Filename:Lamba_Treatment_20150210.docx

Sample Preparation:

Sampleprep ID:SP000316
Sampleprep Summary:See files
Sampleprep Protocol Filename:cell_pellet_preparation.pdf, Appendix C_Protein_DNA_quant.pdf

Combined analysis:

Analysis ID AN000458 AN000459
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area peak area

Chromatography:

Chromatography ID:CH000327
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000398
Analysis ID:AN000458
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:ST286_Metabolomics_LCMSProtocol.pdf
  
MS ID:MS000399
Analysis ID:AN000459
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:ST286_Metabolomics_LCMSProtocol.pdf
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