Summary of Study ST000290

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000232. The data can be accessed directly via it's Project DOI: 10.21228/M8P01G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000290
Study Title2014 Biotron Experiment Metabolites
Study TypeTime course
Study SummaryMaize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.
Institute
University of Florida
DepartmentSECIM
LaboratoryJon
Last NameStewart
First NameJon
Address102 Leigh Hall
Emailjds2@chem.ufl.edu
Phone352-846-0743
Submit Date2015-02-19
Num Groups3
Total Subjects47
Study CommentsNormal day / normal night (17 samples); Hot day / normal night (14 samples); Hot day / hot night (17 samples)
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Jon Stewart Jon Stewart
https://dx.doi.org/10.21228/M8P01G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000232
Project DOI:doi: 10.21228/M8P01G
Project Title:Climate Change and Starch Yield in Maize Part 2
Project Type:MS study
Project Summary:Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.
Institute:University of Florida
Department:Chemistry
Laboratory:Stewart
Last Name:Stewart
First Name:Jon
Address:126 Sisler Hall
Email:jds2@chem.ufl.edu
Phone:846-0743
Funding Source:USDA

Subject:

Subject ID:SU000310
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577
Species Group:Plants

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Temperature Days after pollination
SA013170H_H_plant7_14DAPBoth hot day and hot night 14
SA013171H_H_plant18_14DAPBoth hot day and hot night 14
SA013172H_H_plant16_14DAPBoth hot day and hot night 14
SA013173H_H_plant8_14DAPBoth hot day and hot night 14
SA013174H_H_plant22_18DAPBoth hot day and hot night 18
SA013175H_H_plant17_18DAPBoth hot day and hot night 18
SA013176H_H_plant10_18DAPBoth hot day and hot night 18
SA013177H_H_plant24_22DAPBoth hot day and hot night 22
SA013178H_H_plant2_22DAPBoth hot day and hot night 22
SA013179H_H_plant15_22DAPBoth hot day and hot night 22
SA013180H_H_plant16_26DAPBoth hot day and hot night 26
SA013181H_H_plant14_26DAPBoth hot day and hot night 26
SA013182H_H_plant4_26DAPBoth hot day and hot night 26
SA013183H_H_plant21_30DAPBoth hot day and hot night 30
SA013184H_H_plant23_30DAPBoth hot day and hot night 30
SA013185H_H_plant3_30DAPBoth hot day and hot night 30
SA013186N_N_plant10_14DAPControl 14
SA013187N_N_plant20_14DAPControl 14
SA013188N_N_plant4_14DAPControl 14
SA013189N_N_plant5_18DAPControl 18
SA013190N_N_plant1_18DAPControl 18
SA013191N_N_plant17_18DAPControl 18
SA013192N_N_plant22_22DAPControl 22
SA013193N_N_plant14_22DAPControl 22
SA013194N_N_plant24_22DAPControl 22
SA013195N_N_plant21_22DAPControl 22
SA013196N_N_plant15_26DAPControl 26
SA013197N_N_plant16_26DAPControl 26
SA013198N_N_plant6_26DAPControl 26
SA013199N_N_plant8_26DAPControl 26
SA013200N_N_plant12_30DAPControl 30
SA013201N_N_plant3_30DAPControl 30
SA013202N_N_plant7_30DAPControl 30
SA013203H_N_plant22_14DAPHot day 14
SA013204H_N_plant1_14DAPHot day 14
SA013205H_N_plant2_14DAPHot day 14
SA013206H_N_plant6_18DAPHot day 18
SA013207H_N_plant20_18DAPHot day 18
SA013208H_N_plant23_18DAPHot day 18
SA013209H_N_plant13_22DAPHot day 22
SA013210H_N_plant21_22DAPHot day 22
SA013211H_N_plant4_26DAPHot day 26
SA013212H_N_plant5_26DAPHot day 26
SA013213H_N_plant9_26DAPHot day 26
SA013214H_N_plant17_26DAPHot day 26
SA013215H_N_plant3_30DAPHot day 30
SA013216H_N_plant8_30DAPHot day 30
Showing results 1 to 47 of 47

Collection:

Collection ID:CO000304
Collection Summary:n/a
Sample Type:Maize kernel
Collection Method:manual removal from maize ear
Collection Location:University of Wisconsin Biotron
Storage Conditions:Frozen at -80C

Treatment:

Treatment ID:TR000324
Treatment Summary:n/a
Plant Growth Location:Wisconsin Biotron Facility
Plant Plot Design:Random placement in growth facility
Plant Light Period:16 h per day
Plant Temp:Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C)
Plant Watering Regime:Fully watered
Plant Growth Stage:Varied
Plant Metab Quench Method:Frozen at -80C

Sample Preparation:

Sampleprep ID:SP000318
Sampleprep Summary:n/a
Sampleprep Protocol Filename:Maize_Tissue_sample_prep.pdf

Combined analysis:

Analysis ID AN000462 AN000463
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000329
Methods Filename:Instrument_Parameters.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350 ul/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000402
Analysis ID:AN000462
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Instrument_Parameters.pdf
  
MS ID:MS000403
Analysis ID:AN000463
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Instrument_Parameters.pdf
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