Summary of Study ST000291

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000233. The data can be accessed directly via it's Project DOI: 10.21228/M8J590 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000291
Study TitleLC-MS Based Approaches to Investigate Metabolomic Differences in the Urine of Young Women after Drinking Cranberry Juice or Apple Juice
Study Typedrug dosage
Study SummaryEighteen healthy female college students between 21-29 years old with a normal BMI of 18.5-25 were recruited. Each subject was provided with a list of foods that contained significant amount of procyanidins, such as cranberries, apples, grapes, blueberries, chocolate and plums. They were advised to avoid these foods during the 1-6th day and the rest of the study. On the morning of the 7th day, a first-morning baseline urine sample and blood sample were collected from all human subjects after overnight fasting. Participants were then randomly allocated into two groups (n=9) to consume cranberry juice or apple juice. Six bottles (250 ml/bottle) of juice were given to participants to drink in the morning and evening of the 7th, 8th, and 9th day. On the morning of 10th day, all subjects returned to the clinical unit to provide a first-morning urine sample after overnight fasting. The blood sample was also collected from participants 30 min later after they drank another bottle of juice in the morning. After two-weeks of wash out period, participants switched to the alternative regimen and repeated the protocol. One human subject was dropped off this study because she missed part of her appointments. Another two human subjects were removed from urine metabolomics analyses because they failed to provide required urine samples after juice drinking.The present study aimed to investigate overall metabolic changes caused by procyanidins concentrates from cranberries and apples using a global LCMS based metabolomics approach. All plasma and urine samples were stored at -80oC until analysis.
Institute
University of Florida
DepartmentSECIM
LaboratoryGu
Last NameLiu
First NameHaiyan
Address--
Emailhaiyan66@ufl.edu
Phone352-392-1991x210
Submit Date2014-11-17
Num Groups3
Total Subjects45
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Haiyan Liu Haiyan Liu
https://dx.doi.org/10.21228/M8J590
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000233
Project DOI:doi: 10.21228/M8J590
Project Title:LC-MS Based Approaches to Investigate Metabolomic Differences in the Urine and Plasma of Young Women after Drinking Cranberry Juice or Apple Juice
Project Summary:The present study aimed to investigate overall metabolic changes caused by cranberry juice or apple juice consumption using a global LC-MS based metabolomics approach.
Institute:University of Florida
Department:Food Science and Nutrition
Laboratory:Gu
Last Name:Liu
First Name:Haiyan
Address:--
Email:haiyan66@ufl.edu
Phone:352-392-1991x210

Subject:

Subject ID:SU000311
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:Gu_subjects_human urine.txt
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA013217b15Baseline urine
SA013218b13Baseline urine
SA013219b16Baseline urine
SA013220b17Baseline urine
SA013221b1Baseline urine
SA013222b2Baseline urine
SA013223b12Baseline urine
SA013224b14Baseline urine
SA013225b7Baseline urine
SA013226b11Baseline urine
SA013227b4Baseline urine
SA013228b8Baseline urine
SA013229b6Baseline urine
SA013230b9Baseline urine
SA013231b10Baseline urine
SA013232a8Urine after drinking apple juice
SA013233a9Urine after drinking apple juice
SA013234a7Urine after drinking apple juice
SA013235a6Urine after drinking apple juice
SA013236a10Urine after drinking apple juice
SA013237a1Urine after drinking apple juice
SA013238a4Urine after drinking apple juice
SA013239a2Urine after drinking apple juice
SA013240a16Urine after drinking apple juice
SA013241a17Urine after drinking apple juice
SA013242a15Urine after drinking apple juice
SA013243a14Urine after drinking apple juice
SA013244a12Urine after drinking apple juice
SA013245a13Urine after drinking apple juice
SA013246a11Urine after drinking apple juice
SA013247c10Urine after drinking cranberry juice
SA013248c7Urine after drinking cranberry juice
SA013249c8Urine after drinking cranberry juice
SA013250c6Urine after drinking cranberry juice
SA013251c4Urine after drinking cranberry juice
SA013252c1Urine after drinking cranberry juice
SA013253c2Urine after drinking cranberry juice
SA013254c9Urine after drinking cranberry juice
SA013255c11Urine after drinking cranberry juice
SA013256c15Urine after drinking cranberry juice
SA013257c16Urine after drinking cranberry juice
SA013258c14Urine after drinking cranberry juice
SA013259c13Urine after drinking cranberry juice
SA013260c12Urine after drinking cranberry juice
SA013261c17Urine after drinking cranberry juice
Showing results 1 to 45 of 45

Collection:

Collection ID:CO000305
Collection Summary:n/a
Collection Protocol Filename:Gu_collection_human urine.txt
Sample Type:Urine

Treatment:

Treatment ID:TR000325
Treatment Summary:n/a
Treatment Protocol Filename:Gu_treatment_human urine.txt

Sample Preparation:

Sampleprep ID:SP000319
Sampleprep Summary:n/a
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_urine.pdf

Combined analysis:

Analysis ID AN000464 AN000465
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000330
Methods Filename:Metabolomics_LCMSProtocol_urine.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000404
Analysis ID:AN000464
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_urine.pdf
  
MS ID:MS000405
Analysis ID:AN000465
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_urine.pdf
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