Summary of Study ST000292

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000233. The data can be accessed directly via it's Project DOI: 10.21228/M8J590 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000292
Study TitleLC-MS Based Approaches to Investigate Metabolomic Differences in the Plasma of Young Women after Drinking Cranberry Juice or Apple Juice
Study Typedrug dosage
Study SummaryEighteen healthy female college students between 21-29 years old with a normal BMI of 18.5-25 were recruited. Each subject was provided with a list of foods that contained significant amount of procyanidins, such as cranberries, apples, grapes, blueberries, chocolate and plums. They were advised to avoid these foods during the 1-6th day and the rest of the study. On the morning of the 7th day, a first-morning baseline urine sample and blood sample were collected from all human subjects after overnight fasting. Participants were then randomly allocated into two groups (n=9) to consume cranberry juice or apple juice. Six bottles (250 ml/bottle) of juice were given to participants to drink in the morning and evening of the 7th, 8th, and 9th day. On the morning of 10th day, all subjects returned to the clinical unit to provide a first-morning urine sample after overnight fasting. The blood sample was also collected from participants 30 min later after they drank another bottle of juice in the morning. After two-weeks of wash out period, participants switched to the alternative regimen and repeated the protocol. One human subject was dropped off this study because she missed part of her appointments. Another two human subjects were removed from urine metabolomics analyses because they failed to provide required urine samples after juice drinking.The present study aimed to investigate overall metabolic changes caused by procyanidins concentrates from cranberries and apples using a global LCMS based metabolomics approach. All plasma and urine samples were stored at -80oC until analysis.
Institute
University of Florida
DepartmentSECIM
LaboratoryGu
Last NameLiu
First NameHaiyan
Address--
Emailhaiyan66@ufl.edu
Phone352-392-1991x210
Submit Date2014-11-17
Num Groups3
Total Subjects51
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Haiyan Liu Haiyan Liu
https://dx.doi.org/10.21228/M8J590
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000233
Project DOI:doi: 10.21228/M8J590
Project Title:LC-MS Based Approaches to Investigate Metabolomic Differences in the Urine and Plasma of Young Women after Drinking Cranberry Juice or Apple Juice
Project Summary:The present study aimed to investigate overall metabolic changes caused by cranberry juice or apple juice consumption using a global LC-MS based metabolomics approach.
Institute:University of Florida
Department:Food Science and Nutrition
Laboratory:Gu
Last Name:Liu
First Name:Haiyan
Address:--
Email:haiyan66@ufl.edu
Phone:352-392-1991x210

Subject:

Subject ID:SU000312
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:Gu_subjects_human.txt
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA013262b16Baseline plasma
SA013263b14Baseline plasma
SA013264b13Baseline plasma
SA013265b17Baseline plasma
SA013266b18Baseline plasma
SA013267b1Baseline plasma
SA013268b2Baseline plasma
SA013269b12Baseline plasma
SA013270b15Baseline plasma
SA013271b6Baseline plasma
SA013272b11Baseline plasma
SA013273b3Baseline plasma
SA013274b7Baseline plasma
SA013275b4Baseline plasma
SA013276b8Baseline plasma
SA013277b9Baseline plasma
SA013278b10Baseline plasma
SA013279a8Plasma after drinking apple juice
SA013280a9Plasma after drinking apple juice
SA013281a7Plasma after drinking apple juice
SA013282a4Plasma after drinking apple juice
SA013283a10Plasma after drinking apple juice
SA013284a1Plasma after drinking apple juice
SA013285a3Plasma after drinking apple juice
SA013286a6Plasma after drinking apple juice
SA013287a2Plasma after drinking apple juice
SA013288a16Plasma after drinking apple juice
SA013289a17Plasma after drinking apple juice
SA013290a18Plasma after drinking apple juice
SA013291a15Plasma after drinking apple juice
SA013292a14Plasma after drinking apple juice
SA013293a12Plasma after drinking apple juice
SA013294a13Plasma after drinking apple juice
SA013295a11Plasma after drinking apple juice
SA013296c10Plasma after drinking cranberry juice
SA013297c6Plasma after drinking cranberry juice
SA013298c7Plasma after drinking cranberry juice
SA013299c8Plasma after drinking cranberry juice
SA013300c4Plasma after drinking cranberry juice
SA013301c3Plasma after drinking cranberry juice
SA013302c1Plasma after drinking cranberry juice
SA013303c2Plasma after drinking cranberry juice
SA013304c9Plasma after drinking cranberry juice
SA013305c11Plasma after drinking cranberry juice
SA013306c16Plasma after drinking cranberry juice
SA013307c17Plasma after drinking cranberry juice
SA013308c15Plasma after drinking cranberry juice
SA013309c14Plasma after drinking cranberry juice
SA013310c12Plasma after drinking cranberry juice
SA013311c13Plasma after drinking cranberry juice
SA013312c18Plasma after drinking cranberry juice
Showing results 1 to 51 of 51

Collection:

Collection ID:CO000306
Collection Summary:n/a
Collection Protocol Filename:Gu_collection_human.txt
Sample Type:Blood

Treatment:

Treatment ID:TR000326
Treatment Summary:n/a
Treatment Protocol Filename:Gu_treatment_human.txt

Sample Preparation:

Sampleprep ID:SP000320
Sampleprep Summary:n/a
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000466 AN000467
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000331
Methods Filename:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000406
Analysis ID:AN000466
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000407
Analysis ID:AN000467
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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