Summary of Study ST000293

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000234. The data can be accessed directly via it's Project DOI: 10.21228/M8DG6Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000293
Study TitleVitamin B6 Effects on one-carbon metabolism
Study TypeBefore-and-after
Study SummaryVitamin B6 supplementation with 10 mg/d pyridoxine-HCl for 28-d was given to oral contraceptive (OC) users who initially had vitamin B6 deficiency (PLP < 30 nmol/L). Samples are analyzed before and after supplementation. In addition samples from OC users with low (PLP < 30 nmol/L) , middle (PLP 31-99 nmol/L) and high (PLP > 100 nmol/L) vitamin B6 concentration are compared.
Institute
University of Florida
DepartmentSECIM
LaboratoryFood Science and Human Nutrition
Last NameGregory
First NameJesse
Address--
Emailjfgy@ufl.edu
Phone352-392-1991 ext 225
Submit Date2015-03-13
Num Groups3
Total Subjects72
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Jesse Gregory Jesse Gregory
https://dx.doi.org/10.21228/M8DG6Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000234
Project DOI:doi: 10.21228/M8DG6Q
Project Title:Metabolite profiling before and after vitamin B6 supplementation
Project Summary:Vitamin B6 Effects on one-carbon metabolism
Institute:University of Florida
Department:Food Science and Human Nutrition
Laboratory:Dr. Jesse Gregory
Last Name:Gregory
First Name:Jesse
Address:--
Email:jfgy@ufl.edu
Phone:352-392-1991 ext 225

Subject:

Subject ID:SU000313
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:Gregory_SECIM_Subjects_20150313.txt
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id B6 Status B6 Supplementation
SA013313209164High After
SA013314213304High After
SA013315214880High After
SA013316201676High After
SA013317200332High After
SA013318195259High After
SA013319199594High After
SA013320199917High After
SA013321202201High After
SA013322210061High After
SA013323186289High None
SA013324186385High None
SA013325184585High None
SA013326183601High None
SA013327183112High None
SA013328209037High None
SA013329208069High None
SA013330186428High None
SA013331200484High None
SA013332201343High None
SA013333203467High None
SA013334207652High None
SA013335191485High None
SA013336181435High None
SA013337183111High None
SA013338182346High None
SA013339181896High None
SA013340200912Low Before
SA013341201440Low Before
SA013342199899Low Before
SA013343208767Low Before
SA013344193557Low Before
SA013345212837Low Before
SA013346193152Low Before
SA013347198715Low Before
SA013348214460Low Before
SA013349209363Low Before
SA013350185631Low None
SA013351203581Low None
SA013352208554Low None
SA013353209828Low None
SA013354202463Low None
SA013355200433Low None
SA013356184721Low None
SA013357186373Low None
SA013358197265Low None
SA013359184606Low None
SA013360200967Medium None
SA013361196557Medium None
SA013362196356Medium None
SA013363210166Medium None
SA013364203115Medium None
SA013365197075Medium None
SA013366195024Medium None
SA013367193029Medium None
SA013368181749Medium None
SA013369210190Medium None
SA013370184673Medium None
SA013371190182Medium None
SA013372197255Medium None
SA013373197747Medium None
SA013374203245Medium None
SA013375203440Medium None
SA013376203777Medium None
SA013377203868Medium None
SA013378200215Medium None
SA013379202317Medium None
SA013380198028Medium None
SA013381198557Medium None
SA013382200037Medium None
SA013383202761Medium None
SA013384207787Medium None
Showing results 1 to 72 of 72

Collection:

Collection ID:CO000307
Collection Summary:n/a
Collection Protocol Filename:Gregory_SECIM_Collections_20150313.txt

Treatment:

Treatment ID:TR000327
Treatment Summary:n/a
Treatment Protocol Filename:Gregory_SECIM_Treatment_20150313.txt

Sample Preparation:

Sampleprep ID:SP000321
Sampleprep Summary:-
Sampleprep Protocol Filename:SB_AA_Assay.pdf, SB_OA_Assay.pdf

Combined analysis:

Analysis ID AN000468 AN000469
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Thermo Dionex Ultimate 3000
Column Waters AccQ Tag (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Thermo Quantiva QQQ
Ion Mode POSITIVE POSITIVE
Units uM uM

Chromatography:

Chromatography ID:CH000332
Chromatography Summary:Waters AccQ·Tag Amino Acid Assay
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase
  
Chromatography ID:CH000333
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:45
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS000408
Analysis ID:AN000468
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:SB_AA_Assay.pdf
  
MS ID:MS000409
Analysis ID:AN000469
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:SB_OA_Assay.pdf
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