Summary of Study ST000294

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000235. The data can be accessed directly via it's Project DOI: 10.21228/M88P4S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000294
Study TitleMechanisms of Metabolic Cycles in Diapausing Flesh Fly by Metabolomics Approach
Study Typetime course
Study SummaryInsects use diapause, a programmed period of dormancy, to avoid stressful times of the year and to exploit seasonal times of resource availability. Because most diapausing insects do not feed, they must live off their body reserves for several months and the proper use of metabolic reserves is critical for surviving diapause and performing after diapause termination. Across multiple insects, metabolic depression during diapause has been associated with a switch from aerobic metabolism to facultative anaerobic metabolism, despite insects not suffering environmental oxygen limitation. While metabolic rates are depressed during diapause overall to save energy, some insects show regular cyclical bouts of higher metabolic activity during diapause. The functional importance of these metabolic cycles and the mechanisms underlying these cycles are still unknown, but they may be critical for properly maintaining the balance between energy states and purge the accumulation of anaerobic metabolic byproducts. In the present study, we will test the hypothesis that periodic cycles of increased metabolism during insect diapause are associated with both regenerating organismal energetic states, particularly ATP that may decline during metabolic depression, and for purging metabolites associated with anaerobic metabolism. We will use a combination of non-targeted uHPLC-MS/MS metabolomics and targeted NMR-spectroscopy to identify and quantify metabolites that are altered during the cycles in diapausing pupae of the flesh fly, Sarcophaga crassipalpis. This work will allow us to propose specific biochemical and cellular hypotheses for the regulation of cyclic releases from metabolic depression in diapausing insects. Our work may not only reveal the physiological mechanisms regulating metabolic cycles during diapause in flesh fly, but also provide insight to understand the regulation of similar metabolic cycles in mammalian hibernators (i.e., periodic arousal), and also provide insights into how these cycles could be exploited to disrupt the diapause of insect pests.
Institute
University of Florida
DepartmentSECIM
Last NameChen
First NameChao
AddressDepartment of Entomology and Nematology, Bldg. 970, 1881 Natural Area Dr., Gainesville, FL 32611
Emailchaochenjxau@126.com
Phone352-273-3949
Submit Date2015-03-03
Num Groups5
Total Subjects45
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Chao Chen Chao Chen
https://dx.doi.org/10.21228/M88P4S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000235
Project DOI:doi: 10.21228/M88P4S
Project Title:Mechanisms of Metabolic Cycles in Diapausing Flesh Fly by Metabolomics Approach
Project Type:Time course
Project Summary:Insects use diapause, a programmed period of dormancy, to avoid stressful times of the year and to exploit seasonal times of resource availability. Because most diapausing insects do not feed, they must live off their body reserves for several months and the proper use of metabolic reserves is critical for surviving diapause and performing after diapause termination. Across multiple insects, metabolic depression during diapause has been associated with a switch from aerobic metabolism to facultative anaerobic metabolism, despite insects not suffering environmental oxygen limitation. While metabolic rates are depressed during diapause overall to save energy, some insects show regular cyclical bouts of higher metabolic activity during diapause. The functional importance of these metabolic cycles and the mechanisms underlying these cycles are still unknown, but they may be critical for properly maintaining the balance between energy states and purge the accumulation of anaerobic metabolic byproducts. In the present study, we will test the hypothesis that periodic cycles of increased metabolism during insect diapause are associated with both regenerating organismal energetic states, particularly ATP that may decline during metabolic depression, and for purging metabolites associated with anaerobic metabolism. We will use a combination of non-targeted uHPLC-MS/MS metabolomics and targeted NMR-spectroscopy to identify and quantify metabolites that are altered during the cycles in diapausing pupae of the flesh fly, Sarcophaga crassipalpis. This work will allow us to propose specific biochemical and cellular hypotheses for the regulation of cyclic releases from metabolic depression in diapausing insects. Our work may not only reveal the physiological mechanisms regulating metabolic cycles during diapause in flesh fly, but also provide insight to understand the regulation of similar metabolic cycles in mammalian hibernators (i.e., periodic arousal), and also provide insights into how these cycles could be exploited to disrupt the diapause of insect pests.
Institute:The Institute of Food and Agricultural Sciences
Department:Department of Entomology and Nematology
Last Name:Hahn
First Name:Daniel
Address:P.O. Box 110620 Gainesville FL 32611-0620
Email:dahahn@ufl.edu
Phone:352-273-3968

Subject:

Subject ID:SU000314
Subject Type:Insect
Subject Species:Sarcophaga crassipalpis
Taxonomy ID:59312
Genotype Strain:Lab colony
Age Or Age Range:15-25 day pupae
Weight Or Weight Range:110-130 mg
Animal Animal Supplier:lab colony
Animal Housing:sterilite containers
Animal Light Cycle:10:14 L:D
Animal Feed:beef liver
Animal Inclusion Criteria:must be in diapause
Species Group:Insect

Factors:

Subject type: Insect; Subject species: Sarcophaga crassipalpis (Factor headings shown in green)

mb_sample_id local_sample_id Phase
SA013385B2P304AD
SA013386B2P301AD
SA013387B3P302AD
SA013388B3P303AD
SA013389B3P304AD
SA013390B1P306AD
SA013391B2P302AD
SA013392B1P301AD
SA013393B1P305AD
SA013394B3P103ED
SA013395B3P105ED
SA013396B1P105ED
SA013397B3P106ED
SA013398B2P102ED
SA013399B1P101ED
SA013400B2P103ED
SA013401B1P102ED
SA013402B2P101ED
SA013403B3P504EN
SA013404B3P506EN
SA013405B2P502EN
SA013406B2P504EN
SA013407B1P504EN
SA013408B3P503EN
SA013409B1P506EN
SA013410B1P505EN
SA013411B2P503EN
SA013412B3P404IBA
SA013413B3P403IBA
SA013414B3P402IBA
SA013415B2P403IBA
SA013416B1P406IBA
SA013417B1P403IBA
SA013418B1P402IBA
SA013419B2P402IBA
SA013420B2P401IBA
SA013421B3P205LD
SA013422B3P204LD
SA013423B3P202LD
SA013424B2P201LD
SA013425B2P202LD
SA013426B1P202LD
SA013427B1P205LD
SA013428BEP203LD
SA013429B1P203LD
Showing results 1 to 45 of 45

Collection:

Collection ID:CO000308
Collection Summary:Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction.
Collection Protocol Comments:Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis.
Sample Type:whole fly
Collection Method:flash freezing in liquid nitrogen
Collection Location:Hahn Lab, 3137 Steinmetz
Collection Frequency:Daily
Collection Time:see treatments
Volumeoramount Collected:one pupa per sample
Storage Conditions:-80C
Collection Vials:1.5 ml microcentrifuge tubes
Storage Vials:1.5 ml microcentrifuge tubes
Collection Tube Temp:room temperature

Treatment:

Treatment ID:TR000328
Treatment Summary:ED=Early metabolic depression (48h) LD=Late metabolic depression (144h) AD=Arousing from metabolic depression (6h) IBA=Interbout arousal (15h) EN=Entering metabolic depression (30h)

Sample Preparation:

Sampleprep ID:SP000322
Sampleprep Summary:This sample set is FleshFly tissue. The samples were received dried and frozen. The samples were reconstituted in 1 mL 90:10 Water:Acetonitrile then diluted 10x.

Combined analysis:

Analysis ID AN000470 AN000471
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000334
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000410
Analysis ID:AN000470
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:469_Metabolomics_LCMSProtocol.pdf
  
MS ID:MS000411
Analysis ID:AN000471
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:469_Metabolomics_LCMSProtocol.pdf
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