Summary of Study ST000294
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000235. The data can be accessed directly via it's Project DOI: 10.21228/M88P4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000294 |
Study Title | Mechanisms of Metabolic Cycles in Diapausing Flesh Fly by Metabolomics Approach |
Study Type | time course |
Study Summary | Insects use diapause, a programmed period of dormancy, to avoid stressful times of the year and to exploit seasonal times of resource availability. Because most diapausing insects do not feed, they must live off their body reserves for several months and the proper use of metabolic reserves is critical for surviving diapause and performing after diapause termination. Across multiple insects, metabolic depression during diapause has been associated with a switch from aerobic metabolism to facultative anaerobic metabolism, despite insects not suffering environmental oxygen limitation. While metabolic rates are depressed during diapause overall to save energy, some insects show regular cyclical bouts of higher metabolic activity during diapause. The functional importance of these metabolic cycles and the mechanisms underlying these cycles are still unknown, but they may be critical for properly maintaining the balance between energy states and purge the accumulation of anaerobic metabolic byproducts. In the present study, we will test the hypothesis that periodic cycles of increased metabolism during insect diapause are associated with both regenerating organismal energetic states, particularly ATP that may decline during metabolic depression, and for purging metabolites associated with anaerobic metabolism. We will use a combination of non-targeted uHPLC-MS/MS metabolomics and targeted NMR-spectroscopy to identify and quantify metabolites that are altered during the cycles in diapausing pupae of the flesh fly, Sarcophaga crassipalpis. This work will allow us to propose specific biochemical and cellular hypotheses for the regulation of cyclic releases from metabolic depression in diapausing insects. Our work may not only reveal the physiological mechanisms regulating metabolic cycles during diapause in flesh fly, but also provide insight to understand the regulation of similar metabolic cycles in mammalian hibernators (i.e., periodic arousal), and also provide insights into how these cycles could be exploited to disrupt the diapause of insect pests. |
Institute | University of Florida |
Department | SECIM |
Last Name | Chen |
First Name | Chao |
Address | Department of Entomology and Nematology, Bldg. 970, 1881 Natural Area Dr., Gainesville, FL 32611 |
chaochenjxau@126.com | |
Phone | 352-273-3949 |
Submit Date | 2015-03-03 |
Num Groups | 5 |
Total Subjects | 45 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000235 |
Project DOI: | doi: 10.21228/M88P4S |
Project Title: | Mechanisms of Metabolic Cycles in Diapausing Flesh Fly by Metabolomics Approach |
Project Type: | Time course |
Project Summary: | Insects use diapause, a programmed period of dormancy, to avoid stressful times of the year and to exploit seasonal times of resource availability. Because most diapausing insects do not feed, they must live off their body reserves for several months and the proper use of metabolic reserves is critical for surviving diapause and performing after diapause termination. Across multiple insects, metabolic depression during diapause has been associated with a switch from aerobic metabolism to facultative anaerobic metabolism, despite insects not suffering environmental oxygen limitation. While metabolic rates are depressed during diapause overall to save energy, some insects show regular cyclical bouts of higher metabolic activity during diapause. The functional importance of these metabolic cycles and the mechanisms underlying these cycles are still unknown, but they may be critical for properly maintaining the balance between energy states and purge the accumulation of anaerobic metabolic byproducts. In the present study, we will test the hypothesis that periodic cycles of increased metabolism during insect diapause are associated with both regenerating organismal energetic states, particularly ATP that may decline during metabolic depression, and for purging metabolites associated with anaerobic metabolism. We will use a combination of non-targeted uHPLC-MS/MS metabolomics and targeted NMR-spectroscopy to identify and quantify metabolites that are altered during the cycles in diapausing pupae of the flesh fly, Sarcophaga crassipalpis. This work will allow us to propose specific biochemical and cellular hypotheses for the regulation of cyclic releases from metabolic depression in diapausing insects. Our work may not only reveal the physiological mechanisms regulating metabolic cycles during diapause in flesh fly, but also provide insight to understand the regulation of similar metabolic cycles in mammalian hibernators (i.e., periodic arousal), and also provide insights into how these cycles could be exploited to disrupt the diapause of insect pests. |
Institute: | The Institute of Food and Agricultural Sciences |
Department: | Department of Entomology and Nematology |
Last Name: | Hahn |
First Name: | Daniel |
Address: | P.O. Box 110620 Gainesville FL 32611-0620 |
Email: | dahahn@ufl.edu |
Phone: | 352-273-3968 |
Subject:
Subject ID: | SU000314 |
Subject Type: | Insect |
Subject Species: | Sarcophaga crassipalpis |
Taxonomy ID: | 59312 |
Genotype Strain: | Lab colony |
Age Or Age Range: | 15-25 day pupae |
Weight Or Weight Range: | 110-130 mg |
Animal Animal Supplier: | lab colony |
Animal Housing: | sterilite containers |
Animal Light Cycle: | 10:14 L:D |
Animal Feed: | beef liver |
Animal Inclusion Criteria: | must be in diapause |
Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Sarcophaga crassipalpis (Factor headings shown in green)
mb_sample_id | local_sample_id | Phase |
---|---|---|
SA013385 | B2P304 | AD |
SA013386 | B2P301 | AD |
SA013387 | B3P302 | AD |
SA013388 | B3P303 | AD |
SA013389 | B3P304 | AD |
SA013390 | B1P306 | AD |
SA013391 | B2P302 | AD |
SA013392 | B1P301 | AD |
SA013393 | B1P305 | AD |
SA013394 | B3P103 | ED |
SA013395 | B3P105 | ED |
SA013396 | B1P105 | ED |
SA013397 | B3P106 | ED |
SA013398 | B2P102 | ED |
SA013399 | B1P101 | ED |
SA013400 | B2P103 | ED |
SA013401 | B1P102 | ED |
SA013402 | B2P101 | ED |
SA013403 | B3P504 | EN |
SA013404 | B3P506 | EN |
SA013405 | B2P502 | EN |
SA013406 | B2P504 | EN |
SA013407 | B1P504 | EN |
SA013408 | B3P503 | EN |
SA013409 | B1P506 | EN |
SA013410 | B1P505 | EN |
SA013411 | B2P503 | EN |
SA013412 | B3P404 | IBA |
SA013413 | B3P403 | IBA |
SA013414 | B3P402 | IBA |
SA013415 | B2P403 | IBA |
SA013416 | B1P406 | IBA |
SA013417 | B1P403 | IBA |
SA013418 | B1P402 | IBA |
SA013419 | B2P402 | IBA |
SA013420 | B2P401 | IBA |
SA013421 | B3P205 | LD |
SA013422 | B3P204 | LD |
SA013423 | B3P202 | LD |
SA013424 | B2P201 | LD |
SA013425 | B2P202 | LD |
SA013426 | B1P202 | LD |
SA013427 | B1P205 | LD |
SA013428 | BEP203 | LD |
SA013429 | B1P203 | LD |
Showing results 1 to 45 of 45 |
Collection:
Collection ID: | CO000308 |
Collection Summary: | Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction. |
Collection Protocol Comments: | Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis. |
Sample Type: | whole fly |
Collection Method: | flash freezing in liquid nitrogen |
Collection Location: | Hahn Lab, 3137 Steinmetz |
Collection Frequency: | Daily |
Collection Time: | see treatments |
Volumeoramount Collected: | one pupa per sample |
Storage Conditions: | -80C |
Collection Vials: | 1.5 ml microcentrifuge tubes |
Storage Vials: | 1.5 ml microcentrifuge tubes |
Collection Tube Temp: | room temperature |
Treatment:
Treatment ID: | TR000328 |
Treatment Summary: | ED=Early metabolic depression (48h) LD=Late metabolic depression (144h) AD=Arousing from metabolic depression (6h) IBA=Interbout arousal (15h) EN=Entering metabolic depression (30h) |
Sample Preparation:
Sampleprep ID: | SP000322 |
Sampleprep Summary: | This sample set is FleshFly tissue. The samples were received dried and frozen. The samples were reconstituted in 1 mL 90:10 Water:Acetonitrile then diluted 10x. |
Combined analysis:
Analysis ID | AN000470 | AN000471 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000334 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000410 |
Analysis ID: | AN000470 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |
MS ID: | MS000411 |
Analysis ID: | AN000471 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |