Summary of Study ST000299

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000240. The data can be accessed directly via it's Project DOI: 10.21228/M8N015 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000299
Study TitleConjugated linoleic acid (CLA) study in LDLR-/- mice
Study TypeAcylcarnitine analysis
Study SummaryLDLR-/- mice were fed a high fat high sucrose diet with either 9,11 CLA or 10,12 CLA. Control groups included no supplementation or caloric restriction to mirror weight loss seen in CLA group. Mice were sacrificed and blood was collected into EDTA tubes. Isolated plasma was immediately frozen at -80C. Adipose tissue from gonadal fat (epididymal white adipose tissue, EWAT) and subcutaneous fat (inguinal white adipose tissue, IWAT) and liver were harvested, snap frozen in liquid nitrogen, and immediately frozen at -80C. An aliquot of thawed plasma was prepared for this project and frozen in an eppendorf tube. Small pieces of frozen tissue were cut and weighed on dry ice and packaged in individual foil packets for this project. **Note: tissue weight is approximate**
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-02-25
Num Groups4
Total Subjects80
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8N015
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000240
Project DOI:doi: 10.21228/M8N015
Project Title:Conjugated linoleic acid (CLA) metabolomics
Project Summary:Effects of CLA on lipid metabolism in obese mice
Institute:University of Washington
Department:Medicine/Metabolism
Laboratory:Chait Lab
Last Name:den Hartigh
First Name:Laura
Address:Seattle, WA
Email:lauradh@uw.edu
Phone:206-543-2264

Subject:

Subject ID:SU000319
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample type Diet modification
SA013524S00017465Epididymal white adipose tissue 10,12-conjugated linoleic acid
SA013525S00017462Epididymal white adipose tissue 10,12-conjugated linoleic acid
SA013526S00017463Epididymal white adipose tissue 10,12-conjugated linoleic acid
SA013527S00017464Epididymal white adipose tissue 10,12-conjugated linoleic acid
SA013528S00017461Epididymal white adipose tissue 10,12-conjugated linoleic acid
SA013529S00017452Epididymal white adipose tissue 9,11-conjugated linoleic acid
SA013530S00017451Epididymal white adipose tissue 9,11-conjugated linoleic acid
SA013531S00017453Epididymal white adipose tissue 9,11-conjugated linoleic acid
SA013532S00017455Epididymal white adipose tissue 9,11-conjugated linoleic acid
SA013533S00017454Epididymal white adipose tissue 9,11-conjugated linoleic acid
SA013534S00017460Epididymal white adipose tissue Caloric restriction
SA013535S00017459Epididymal white adipose tissue Caloric restriction
SA013536S00017457Epididymal white adipose tissue Caloric restriction
SA013537S00017456Epididymal white adipose tissue Caloric restriction
SA013538S00017458Epididymal white adipose tissue Caloric restriction
SA013539S00017467Epididymal white adipose tissue None
SA013540S00017470Epididymal white adipose tissue None
SA013541S00017466Epididymal white adipose tissue None
SA013542S00017469Epididymal white adipose tissue None
SA013543S00017468Epididymal white adipose tissue None
SA013544S00017483Inguinal white adipose tissue 10,12-conjugated linoleic acid
SA013545S00017481Inguinal white adipose tissue 10,12-conjugated linoleic acid
SA013546S00017485Inguinal white adipose tissue 10,12-conjugated linoleic acid
SA013547S00017482Inguinal white adipose tissue 10,12-conjugated linoleic acid
SA013548S00017484Inguinal white adipose tissue 10,12-conjugated linoleic acid
SA013549S00017474Inguinal white adipose tissue 9,11-conjugated linoleic acid
SA013550S00017472Inguinal white adipose tissue 9,11-conjugated linoleic acid
SA013551S00017473Inguinal white adipose tissue 9,11-conjugated linoleic acid
SA013552S00017475Inguinal white adipose tissue 9,11-conjugated linoleic acid
SA013553S00017471Inguinal white adipose tissue 9,11-conjugated linoleic acid
SA013554S00017476Inguinal white adipose tissue Caloric restriction
SA013555S00017479Inguinal white adipose tissue Caloric restriction
SA013556S00017478Inguinal white adipose tissue Caloric restriction
SA013557S00017477Inguinal white adipose tissue Caloric restriction
SA013558S00017480Inguinal white adipose tissue Caloric restriction
SA013559S00017487Inguinal white adipose tissue None
SA013560S00017490Inguinal white adipose tissue None
SA013561S00017489Inguinal white adipose tissue None
SA013562S00017488Inguinal white adipose tissue None
SA013563S00017486Inguinal white adipose tissue None
SA013564S00017501Liver 10,12-conjugated linoleic acid
SA013565S00017503Liver 10,12-conjugated linoleic acid
SA013566S00017502Liver 10,12-conjugated linoleic acid
SA013567S00017504Liver 10,12-conjugated linoleic acid
SA013568S00017505Liver 10,12-conjugated linoleic acid
SA013569S00017493Liver 9,11-conjugated linoleic acid
SA013570S00017494Liver 9,11-conjugated linoleic acid
SA013571S00017495Liver 9,11-conjugated linoleic acid
SA013572S00017492Liver 9,11-conjugated linoleic acid
SA013573S00017491Liver 9,11-conjugated linoleic acid
SA013574S00017498Liver Caloric restriction
SA013575S00017499Liver Caloric restriction
SA013576S00017500Liver Caloric restriction
SA013577S00017497Liver Caloric restriction
SA013578S00017496Liver Caloric restriction
SA013579S00017508Liver None
SA013580S00017507Liver None
SA013581S00017506Liver None
SA013582S00017509Liver None
SA013583S00017510Liver None
SA013584S00017442Plasma 10,12-conjugated linoleic acid
SA013585S00017443Plasma 10,12-conjugated linoleic acid
SA013586S00017445Plasma 10,12-conjugated linoleic acid
SA013587S00017444Plasma 10,12-conjugated linoleic acid
SA013588S00017441Plasma 10,12-conjugated linoleic acid
SA013589S00017432Plasma 9,11-conjugated linoleic acid
SA013590S00017431Plasma 9,11-conjugated linoleic acid
SA013591S00017435Plasma 9,11-conjugated linoleic acid
SA013592S00017434Plasma 9,11-conjugated linoleic acid
SA013593S00017433Plasma 9,11-conjugated linoleic acid
SA013594S00017436Plasma Caloric restriction
SA013595S00017437Plasma Caloric restriction
SA013596S00017440Plasma Caloric restriction
SA013597S00017439Plasma Caloric restriction
SA013598S00017438Plasma Caloric restriction
SA013599S00017448Plasma None
SA013600S00017447Plasma None
SA013601S00017450Plasma None
SA013602S00017446Plasma None
SA013603S00017449Plasma None
Showing results 1 to 80 of 80

Collection:

Collection ID:CO000313
Collection Summary:-
Sample Type:Blood, tissues

Treatment:

Treatment ID:TR000333
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000327
Sampleprep Summary:-
Sampleprep Protocol Filename:AcylCarnitines_-_20150305.docx

Combined analysis:

Analysis ID AN000477
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters X BridgeC18 (50 x 1mm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000340
Methods Filename:QM_004XBridge2mm_ACAR_MRM20150412.m.zip
Instrument Name:Agilent 1260
Column Name:Waters X BridgeC18 (50 x 1mm)
Chromatography Type:Reversed phase

MS:

MS ID:MS000417
Analysis ID:AN000477
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:AcylCarnitines_-_20150305.docx
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