Summary of Study ST000300
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000241. The data can be accessed directly via it's Project DOI: 10.21228/M8H88B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000300 |
| Study Title | Chicken/C. jejuni Bile Acid Study |
| Study Summary | Cecal samples were isolated directly from the ceca of dissected chickens that were either experimentally infected with C. jejuni DRH212 or mock-infected with PBS. Cecal samples were re-suspended in Life Technologies 1X PBS based on weight of sample (1 ml/100 mg = 10-1 dilution) and stored at -80C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2015-03-17 |
| Num Groups | 2 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000241 |
| Project DOI: | doi: 10.21228/M8H88B |
| Project Title: | Identifiying Colonization Factors of Campylobacter jejuni |
| Project Summary: | Using next-generation approaches to identify both bacterial and host factors that influence colonization of the chicken gastrointestinal tract by C. jejuni |
| Institute: | University of Michigan |
| Department: | Microbiology and Immunology |
| Laboratory: | DiRita Lab |
| Last Name: | DiRita |
| First Name: | Victor |
| Address: | Ann Arbor, MI |
| Email: | vdirita@umich.edu |
| Phone: | 734-936-3804 |
Subject:
| Subject ID: | SU000320 |
| Subject Type: | Animal |
| Subject Species: | Gallus gallus |
| Taxonomy ID: | 9031 |
| Species Group: | Birds |
Factors:
Subject type: Animal; Subject species: Gallus gallus (Factor headings shown in green)
| mb_sample_id | local_sample_id | C. jejuni Infection |
|---|---|---|
| SA013604 | S00017777 | no |
| SA013605 | S00017776 | no |
| SA013606 | S00017775 | yes |
| SA013607 | S00017774 | yes |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000314 |
| Collection Summary: | - |
| Sample Type: | Cecum |
Treatment:
| Treatment ID: | TR000334 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000328 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Bile Acids-20150305.docx |
Combined analysis:
| Analysis ID | AN000478 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1260 |
| Column | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410 QQQ |
| Ion Mode | NEGATIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000341 |
| Methods Filename: | 2014-07-30-Bile_acid-full-BEH-C18-I.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000418 |
| Analysis ID: | AN000478 |
| Instrument Name: | Agilent 6410 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Bile_Acids-20150305.docx |
