Summary of Study ST000301
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000242. The data can be accessed directly via it's Project DOI: 10.21228/M8CG6D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000301 |
| Study Title | GBM Cell Lines Reproducibility Pilot Study |
| Study Summary | GBM cell lines were plated onto 10 cm dishes at 100,000-200,000 cells per dish and allowed to grow for 3-4 days until confluency reached 50-70%. Media for all cell lines was DMEM with 10% FBS including 25 mM glucose, 6.2 mM glutamine and 200 uM Oleic Acid (conjugated to BSA). 1-2 hours prior to tracer incubation, media was aspirated and fresh media was used. Immediately prior to tracer incubation, media was again aspirated and then cells were washed with warm PBS to remove unlabeled metabolites. Cells were then incubated with labeled tracer (DMEM with 10% FBS including 25 mM Uniformly labeled 13C-6 glucose, 6.2 mM unlabeled glutamine and 200 uM unlabeled oleic acid). After 2 hours of incubation with tracer, media was aspirated, cells were quickly washed with 15 mL DI water, quenched with liquid nitrogen and then stored at -80oC until analysis. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2015-03-17 |
| Num Groups | 10 |
| Total Subjects | 28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000242 |
| Project DOI: | doi: 10.21228/M8CG6D |
| Project Title: | DRW GBM Cell Line Metabolomics |
| Project Summary: | Profiling 13C glucose flux-based metabolome across GBM cell lines to determine metabolic predictors of radiation sensitivity |
| Institute: | University of Michigan |
| Department: | Radiation Oncology |
| Last Name: | Lawrence |
| First Name: | Theodore |
| Address: | Ann Arbor, MI |
| Email: | tsl@umich.edu |
| Phone: | 734-647-9955 |
Subject:
| Subject ID: | SU000321 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell Line |
|---|---|---|
| SA013608 | S00017760 | L229 |
| SA013609 | S00017768 | L229 |
| SA013610 | S00017770 | L229 |
| SA013611 | S00017759 | L229 |
| SA013612 | S00017769 | L229 |
| SA013613 | S00017761 | L229 |
| SA013614 | S00017752 | L229 |
| SA013615 | S00017750 | L229 |
| SA013616 | S00017751 | L229 |
| SA013617 | S00017754 | LN18 |
| SA013618 | S00017771 | LN18 |
| SA013619 | S00017773 | LN18 |
| SA013620 | S00017772 | LN18 |
| SA013621 | S00017763 | LN18 |
| SA013622 | S00017764 | LN18 |
| SA013623 | S00017762 | LN18 |
| SA013624 | S00017753 | LN18 |
| SA013625 | S00017755 | LN18 |
| SA013626 | S00020346 | Pooled glioblastoma |
| SA013627 | S00017748 | U-138 |
| SA013628 | S00017756 | U-138 |
| SA013629 | S00017757 | U-138 |
| SA013630 | S00017758 | U-138 |
| SA013631 | S00017747 | U-138 |
| SA013632 | S00017765 | U-138 |
| SA013633 | S00017766 | U-138 |
| SA013634 | S00017767 | U-138 |
| SA013635 | S00017749 | U-138 |
| Showing results 1 to 28 of 28 |
Collection:
| Collection ID: | CO000315 |
| Collection Summary: | - |
| Sample Type: | Glioblastoma cells |
Treatment:
| Treatment ID: | TR000335 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000329 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000479 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000342 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm_bottompheater-no_insert.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000419 |
| Analysis ID: | AN000479 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Fluxomics_analysis_protocol-2015-03-11.docx |
