Summary of Study ST000304

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000244. The data can be accessed directly via it's Project DOI: 10.21228/M87S34 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000304
Study TitleEarly in life exposure studies on human and mouse samples
Study SummaryExperiment1: 2 day old baby mice were exposed to hyperoxia (75% O2) continuously for 7 days. Control baby mice were housed in room air (normoxia). Plasma and bronchoalveolar lavage fluid (BALF) were harvested after 7 days of exposure (on Day of life 9). Experiment2: 2 day old baby mice were exposed to room air or hyperoxia for 14 days and subsequently treated with RV1 or sham.Plasma was collected 5 days after treatment. Human tracheal aspirates were collected from prematurely born infants undergoing mechanical ventilation for respiratory distress syndrome in the first week of life.Tracheal aspirate supernatants are submitted for the assay. We would like to measure adenosine, AMP, ADP and ATP levels.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-26
Num Groups7
Total Subjects52
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M87S34
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000244
Project DOI:doi: 10.21228/M87S34
Project Title:Early in life exposure studies
Project Summary:Early in life exposures affect innate immune cells activation
Institute:University of Michigan
Department:Pediatrics
Laboratory:Hershenson Lab
Last Name:Popova
First Name:Antonia
Address:Ann Arbor, MI
Email:antoniap@med.umich.edu
Phone:734-764-6736

Subject:

Subject ID:SU000324
Subject Type:Human/animal
Subject Species:Mus musculus;Homo sapiens
Taxonomy ID:10090;9606
Species Group:Mammals

Factors:

Subject type: Human/animal; Subject species: Mus musculus;Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Hyperoxia exposure RV infection Sample type
SA013705S00017939n/a n/a Trachea
SA013706S00017938n/a n/a Trachea
SA013707S00017933n/a n/a Trachea
SA013708S00017932n/a n/a Trachea
SA013709S00017934n/a n/a Trachea
SA013710S00017940n/a n/a Trachea
SA013711S00017935n/a n/a Trachea
SA013712S00017941n/a n/a Trachea
SA013713S00017936n/a n/a Trachea
SA013714S00017937n/a n/a Trachea
SA013715S00017907n n/a Bronchoalveolar Lavage Fluid
SA013716S00017908n n/a Bronchoalveolar Lavage Fluid
SA013717S00017906n n/a Bronchoalveolar Lavage Fluid
SA013718S00017901n n/a Bronchoalveolar Lavage Fluid
SA013719S00017898n n/a Bronchoalveolar Lavage Fluid
SA013720S00017899n n/a Bronchoalveolar Lavage Fluid
SA013721S00017900n n/a Bronchoalveolar Lavage Fluid
SA013722S00017893n n/a Plasma
SA013723S00017890n n/a Plasma
SA013724S00017891n n/a Plasma
SA013725S00017892n n/a Plasma
SA013726S00017913n n/a Plasma
SA013727S00017912n n/a Plasma
SA013728S00017911n n/a Plasma
SA013729S00017919n n Plasma
SA013730S00017920n n Plasma
SA013731S00017918n n Plasma
SA013732S00017917n n Plasma
SA013733S00017925n y Plasma
SA013734S00017927n y Plasma
SA013735S00017928n y Plasma
SA013736S00017926n y Plasma
SA013737S00017909y n/a Bronchoalveolar Lavage Fluid
SA013738S00017902y n/a Bronchoalveolar Lavage Fluid
SA013739S00017903y n/a Bronchoalveolar Lavage Fluid
SA013740S00017904y n/a Bronchoalveolar Lavage Fluid
SA013741S00017905y n/a Bronchoalveolar Lavage Fluid
SA013742S00017910y n/a Bronchoalveolar Lavage Fluid
SA013743S00017916y n/a Plasma
SA013744S00017897y n/a Plasma
SA013745S00017914y n/a Plasma
SA013746S00017896y n/a Plasma
SA013747S00017895y n/a Plasma
SA013748S00017894y n/a Plasma
SA013749S00017915y n/a Plasma
SA013750S00017921y n Plasma
SA013751S00017922y n Plasma
SA013752S00017923y n Plasma
SA013753S00017931y y Plasma
SA013754S00017929y y Plasma
SA013755S00017924y y Plasma
SA013756S00017930y y Plasma
Showing results 1 to 52 of 52

Collection:

Collection ID:CO000318
Collection Summary:-
Sample Type:Bronchoalveolar Lavage Fluid

Treatment:

Treatment ID:TR000338
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000332
Sampleprep Summary:-
Sampleprep Protocol Filename:Glycolysis-TCA-nucleotides-20150324.docx

Combined analysis:

Analysis ID AN000483
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode NEGATIVE
Units nM

Chromatography:

Chromatography ID:CH000346
Methods Filename:QQQ-ATP,ADP,AMP,A-update.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000423
Analysis ID:AN000483
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Glycolysis-TCA-nucleotides-20150324.docx
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