Summary of Study ST000310

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000249. The data can be accessed directly via it's Project DOI: 10.21228/M8M01V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000310
Study TitleTC and B6 untreated plasma in lupus-prone mice lipidomics (part-II)
Study Typeuntreated
Study Summarycompare plasma samples from 3 month old lupus-prone (TC) and control (B6) mice
Institute
University of Florida
DepartmentSECIM
LaboratoryLaurence M. Morel Laboratory
Last NameMorel
First NameLaurence
AddressCollege of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Emailmorel@ufl.edu
Phone352-392-3790
Submit Date2016-01-17
Num Groups2
Total Subjects17
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2016-01-18
Release Version1
Laurence Morel Laurence Morel
https://dx.doi.org/10.21228/M8M01V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000249
Project DOI:doi: 10.21228/M8M01V
Project Title:Lupus lipidomics
Project Summary:The purpose of this study was to compare the metabolic and lipidomic profile of plasma from 3 month old lupus-prone (TC) and control (B6) mice.
Institute:University of Florida
Department:Pathology, Immunology and Laboratory Medicine
Laboratory:Laurence M. Morel Laboratory
Last Name:Morel
First Name:Laurence
Address:College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Email:morel@ufl.edu
Phone:352-392-3790

Subject:

Subject ID:SU000330
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment
SA014047TC_f3649_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014048TC_f3649B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014049TC_f3646_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014050TC_f3651B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014051TC_f3651_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014052TC_f3668_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014053TC_f3668B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014054TC_f3646B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014055B6_F5_2C57BL/6J control
SA014056B6_F3C57BL/6J control
SA014057B6_F2C57BL/6J control
SA014058B6_F1_2C57BL/6J control
SA014059B6_F3_2C57BL/6J control
SA014060B6_F4C57BL/6J control
SA014061B6_F5C57BL/6J control
SA014062B6_F4_2C57BL/6J control
SA014063B6_F1C57BL/6J control
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000324
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000344
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000338
Sampleprep Summary:None
Sampleprep Protocol Filename:Lipidomics_LCMSProtocol.pdf

Combined analysis:

Analysis ID AN000490 AN000491 AN000492 AN000493
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak height Peak height

Chromatography:

Chromatography ID:CH000350
Chromatography Summary:None
Methods Filename:Lipidomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000428
Analysis ID:AN000490
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Area
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000429
Analysis ID:AN000491
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Area
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000430
Analysis ID:AN000492
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Height
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000431
Analysis ID:AN000493
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Height
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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