Summary of Study ST000311

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000250. The data can be accessed directly via it's Project DOI: 10.21228/M8G881 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000311
Study TitleTC and B6 untreated plasma in lupus-prone mice
Study Typeuntreated
Study Summarycompare plasma samples from 3 month old lupus-prone (TC) and control (B6) mice
Institute
University of Florida
DepartmentSECIM
LaboratoryLaurence M. Morel Laboratory
Last NameMorel
First NameLaurence
AddressCollege of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Emailmorel@ufl.edu
Phone352-392-3790
Submit Date2016-01-17
Num Groups2
Total Subjects17
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-02-01
Release Version1
Laurence Morel Laurence Morel
https://dx.doi.org/10.21228/M8G881
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000250
Project DOI:doi: 10.21228/M8G881
Project Title:Lupus metabolomics
Project Summary:The purpose of this study was to compare the metabolic and lipidomic profile of plasma from 3 month old lupus-prone (TC) and control (B6) mice.
Institute:University of Florida
Department:Pathology, Immunology and Laboratory Medicine
Laboratory:Laurence M. Morel Laboratory
Last Name:Morel
First Name:Laurence
Address:College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Email:morel@ufl.edu
Phone:352-392-3790

Subject:

Subject ID:SU000331
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment
SA014064TC_f3649_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014065TC_f3649B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014066TC_f3646_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014067TC_f3651B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014068TC_f3651_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014069TC_f3668_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014070TC_f3668B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014071TC_f3646B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014072B6_F5_2C57BL/6J control
SA014073B6_F3C57BL/6J control
SA014074B6_F2C57BL/6J control
SA014075B6_F1_2C57BL/6J control
SA014076B6_F3_2C57BL/6J control
SA014077B6_F4C57BL/6J control
SA014078B6_F5C57BL/6J control
SA014079B6_F4_2C57BL/6J control
SA014080B6_F1C57BL/6J control
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000325
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Collection Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000345
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000339
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000494 AN000495 AN000496 AN000497
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak height Peak height

Chromatography:

Chromatography ID:CH000351
Chromatography Summary:None
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000432
Analysis ID:AN000494
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Area
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000433
Analysis ID:AN000495
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Area
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000434
Analysis ID:AN000496
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Height
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000435
Analysis ID:AN000497
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Height
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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