Summary of Study ST000311
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000250. The data can be accessed directly via it's Project DOI: 10.21228/M8G881 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000311 |
Study Title | TC and B6 untreated plasma in lupus-prone mice |
Study Type | untreated |
Study Summary | compare plasma samples from 3 month old lupus-prone (TC) and control (B6) mice |
Institute | University of Florida |
Department | SECIM |
Laboratory | Laurence M. Morel Laboratory |
Last Name | Morel |
First Name | Laurence |
Address | College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275 |
morel@ufl.edu | |
Phone | 352-392-3790 |
Submit Date | 2016-01-17 |
Num Groups | 2 |
Total Subjects | 17 |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-02-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000250 |
Project DOI: | doi: 10.21228/M8G881 |
Project Title: | Lupus metabolomics |
Project Summary: | The purpose of this study was to compare the metabolic and lipidomic profile of plasma from 3 month old lupus-prone (TC) and control (B6) mice. |
Institute: | University of Florida |
Department: | Pathology, Immunology and Laboratory Medicine |
Laboratory: | Laurence M. Morel Laboratory |
Last Name: | Morel |
First Name: | Laurence |
Address: | College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275 |
Email: | morel@ufl.edu |
Phone: | 352-392-3790 |
Subject:
Subject ID: | SU000331 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | [C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)] |
Age Or Age Range: | 99 days/93-99 days |
Gender: | female |
Animal Animal Supplier: | in house/in house |
Animal Housing: | SPF/SPF |
Animal Light Cycle: | standard|standard |
Animal Feed: | standard|standard |
Animal Water: | standard|standard |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Strain | Treatment |
---|---|---|---|
SA014064 | TC_f3649_2 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014065 | TC_f3649 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014066 | TC_f3646_2 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014067 | TC_f3651 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014068 | TC_f3651_2 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014069 | TC_f3668_2 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014070 | TC_f3668 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014071 | TC_f3646 | B6.Sle1.Sle2.Sle3 (TC) | lupus prone |
SA014072 | B6_F5_2 | C57BL/6J | control |
SA014073 | B6_F3 | C57BL/6J | control |
SA014074 | B6_F2 | C57BL/6J | control |
SA014075 | B6_F1_2 | C57BL/6J | control |
SA014076 | B6_F3_2 | C57BL/6J | control |
SA014077 | B6_F4 | C57BL/6J | control |
SA014078 | B6_F5 | C57BL/6J | control |
SA014079 | B6_F4_2 | C57BL/6J | control |
SA014080 | B6_F1 | C57BL/6J | control |
Showing results 1 to 17 of 17 |
Collection:
Collection ID: | CO000325 |
Collection Summary: | None |
Collection Protocol ID: | IACUC 201308189 |
Collection Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Sample Type: | blood |
Collection Method: | heart puncture |
Collection Time: | terminal |
Storage Conditions: | -20C |
Collection Vials: | heparinized tubes |
Blood Serum Or Plasma: | plasma |
Treatment:
Treatment ID: | TR000345 |
Treatment Summary: | no treatement (controls for KD diet study) |
Sample Preparation:
Sampleprep ID: | SP000339 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
Analysis ID | AN000494 | AN000495 | AN000496 | AN000497 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000351 |
Chromatography Summary: | None |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000432 |
Analysis ID: | AN000494 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI Positive Peak Area |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000433 |
Analysis ID: | AN000495 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI Negative Peak Area |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000434 |
Analysis ID: | AN000496 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI Positive Peak Height |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000435 |
Analysis ID: | AN000497 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI Negative Peak Height |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |