Summary of Study ST000312
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000251. The data can be accessed directly via it's Project DOI: 10.21228/M8BG63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000312 |
| Study Title | IDH1R132H activity in glioma cell lines and tumnor tissue (2HG) |
| Study Type | Regular |
| Study Summary | We developed genetically engineered mice to generate brain tumors with especific genetic lessions. The animals were split in three groups: NRAS, P53 knockdown, IDH1-R132H and ATRX knock down (NPAID); NRAS, P53 knockdown, IDH1-R132H (NPI); NRAS, P53 knockdown, (NshP53). From these tumor we obtain and culture tumor cells growth like neurospheres and attached cells. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2015-03-05 |
| Num Groups | 4 |
| Total Subjects | 4 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000251 |
| Project DOI: | doi: 10.21228/M8BG63 |
| Project Title: | IDH1 gliomas |
| Project Summary: | Analyze IDH1R132H activity in glioma cell lines and tumnor tissue |
| Institute: | University of Michigan |
| Department: | Neurosurgery |
| Laboratory: | Castro Lab |
| Last Name: | Castro |
| First Name: | Maria |
| Address: | Ann Arbor, MI |
| Email: | mariacas@umich.edu |
| Phone: | 734-764-0850 |
Subject:
| Subject ID: | SU000332 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genetic makeup |
|---|---|---|
| SA014081 | S00017646 | NPAID |
| SA014082 | S00017647 | NPI |
| SA014083 | S00017648 | NshP53 |
| SA014084 | S00020347 | Pooled cells |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000326 |
| Collection Summary: | None provided. |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000346 |
| Treatment Summary: | None provided. |
Sample Preparation:
| Sampleprep ID: | SP000340 |
| Sampleprep Summary: | None provided. |
| Sampleprep Protocol Filename: | 15DEC2015_2HG_cells_Castro.docx |
Combined analysis:
| Analysis ID | AN000498 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Unspecified |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410A QQQ |
| Ion Mode | NEGATIVE |
| Units | µM/500µl extraction solvent |
Chromatography:
| Chromatography ID: | CH000352 |
| Methods ID: | DQM0033 |
| Methods Filename: | 2015-04-07-2HG.m.zip |
| Instrument Name: | Unspecified |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000436 |
| Analysis ID: | AN000498 |
| Instrument Name: | Agilent 6410A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
