Summary of Study ST000312
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000251. The data can be accessed directly via it's Project DOI: 10.21228/M8BG63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000312 |
Study Title | IDH1R132H activity in glioma cell lines and tumnor tissue (2HG) |
Study Type | Regular |
Study Summary | We developed genetically engineered mice to generate brain tumors with especific genetic lessions. The animals were split in three groups: NRAS, P53 knockdown, IDH1-R132H and ATRX knock down (NPAID); NRAS, P53 knockdown, IDH1-R132H (NPI); NRAS, P53 knockdown, (NshP53). From these tumor we obtain and culture tumor cells growth like neurospheres and attached cells. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2015-03-05 |
Num Groups | 4 |
Total Subjects | 4 |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-06-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000251 |
Project DOI: | doi: 10.21228/M8BG63 |
Project Title: | IDH1 gliomas |
Project Summary: | Analyze IDH1R132H activity in glioma cell lines and tumnor tissue |
Institute: | University of Michigan |
Department: | Neurosurgery |
Laboratory: | Castro Lab |
Last Name: | Castro |
First Name: | Maria |
Address: | Ann Arbor, MI |
Email: | mariacas@umich.edu |
Phone: | 734-764-0850 |
Subject:
Subject ID: | SU000332 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Genetic makeup |
---|---|---|
SA014081 | S00017646 | NPAID |
SA014082 | S00017647 | NPI |
SA014083 | S00017648 | NshP53 |
SA014084 | S00020347 | Pooled cells |
Showing results 1 to 4 of 4 |
Collection:
Collection ID: | CO000326 |
Collection Summary: | None provided. |
Sample Type: | Cells |
Treatment:
Treatment ID: | TR000346 |
Treatment Summary: | None provided. |
Sample Preparation:
Sampleprep ID: | SP000340 |
Sampleprep Summary: | None provided. |
Sampleprep Protocol Filename: | 15DEC2015_2HG_cells_Castro.docx |
Combined analysis:
Analysis ID | AN000498 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Unspecified |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410A QQQ |
Ion Mode | NEGATIVE |
Units | µM/500µl extraction solvent |
Chromatography:
Chromatography ID: | CH000352 |
Methods ID: | DQM0033 |
Methods Filename: | 2015-04-07-2HG.m.zip |
Instrument Name: | Unspecified |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000436 |
Analysis ID: | AN000498 |
Instrument Name: | Agilent 6410A QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |