Summary of Study ST000313
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000252. The data can be accessed directly via it's Project DOI: 10.21228/M86S3T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000313 |
| Study Title | Muscle Clock knock out mice metabolic changes (iMSBmal1-Exp1) |
| Study Type | Regular |
| Study Summary | My lab studies the function of the molecular clocks in skeletal muscle. We have an inducible genetic mouse model (C57Bl6 background) in which we knock out the core clock gene, Bmal1, only in adult skeletal muscle after treatment with tamoxifen. We have found that the mice maintain body mass but lose fat mass at 10 weeks after loss of Bmal1. We have done expression profiling on the skeletal muscles and gene expression changes (insulin signaling, CHO metabolism, fat metabolism) suggest significant changes in substrate metabolism. To analyze TCA, CHO metabolites we have collected gastrocnemius muscles from these mice following instructions from Dr. Burant. Mice were anaesthetized with isoflurane, the gastrocnemius muscle dissected and flash frozen with tongs cooled with liquid N2. They have been stored in cryovials in our -80 freezer for 2 months. |
| Institute | University of Michigan |
| Department | Biomedical Research Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2015-03-26 |
| Num Groups | 2 |
| Total Subjects | 14 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000252 |
| Project DOI: | doi: 10.21228/M86S3T |
| Project Title: | Muscle Clock knock out metabolic changes |
| Project Summary: | Inducible KO of Bmal1 in skeletal muscle; we see transcriptional changes suggesting significant effects in TCA/substrate metabolism |
| Institute: | University of Kentucky |
| Department: | Physiology |
| Laboratory: | Esser Lab |
| Last Name: | Esser |
| First Name: | Karyn |
| Address: | Lexington, KY |
| Email: | karyn.esser@uky.edu |
| Phone: | 859-323-8107 |
Subject:
| Subject ID: | SU000333 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | muscle Bmal1 |
|---|---|---|
| SA014085 | S00017952 | Bmal1 negative |
| SA014086 | S00017951 | Bmal1 negative |
| SA014087 | S00017953 | Bmal1 negative |
| SA014088 | S00017954 | Bmal1 negative |
| SA014089 | S00017955 | Bmal1 negative |
| SA014090 | S00017950 | Bmal1 negative |
| SA014091 | S00017949 | Bmal1 negative |
| SA014092 | S00017944 | Bmal1 positive |
| SA014093 | S00017943 | Bmal1 positive |
| SA014094 | S00017945 | Bmal1 positive |
| SA014095 | S00017946 | Bmal1 positive |
| SA014096 | S00017948 | Bmal1 positive |
| SA014097 | S00017947 | Bmal1 positive |
| SA014098 | S00017942 | Bmal1 positive |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000327 |
| Collection Summary: | None |
| Sample Type: | Muscle, Skeletal |
Treatment:
| Treatment ID: | TR000347 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000341 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Glycolysis-TCA-nucleotides-20150324.docx |
Combined analysis:
| Analysis ID | AN000499 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE |
| Units | µM |
Chromatography:
| Chromatography ID: | CH000353 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm_bottompheater.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000437 |
| Analysis ID: | AN000499 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
