Summary of Study ST000316

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000255. The data can be accessed directly via it's Project DOI: 10.21228/M8TG7T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000316
Study TitleComparison of Metabolites Variation and Antiobesity Effects of a Mixture of Cudrania tricuspidata, Lonicera caerulea, and the Soybean According to Fermentation in vitro and in vivo
Study SummaryWe used ultra-performance-liquid-chromatography with quadrupole-time-of-flight mass spectrometry to study the changes in metabolites in the mixture of Cudrania tricuspidata, Lonicera caerulea, and soybean (CLM) during fermentation. Additionally, the antiobesity effects of CLM and fermented-CLM (FCLM) were studied based on the analysis of plasma from high-fat diet (HFD)-fed mice. The levels of cyanidin and the glycosides of luteolin, quercetin, and cyanidin derived from L. caerulea were decreased, whereas the levels of luteolin and quercetin were increased during fermentation. Isoflavone glycosides and soyasaponins originating from the soybean were decreased, whereas their aglycones such as daidzein, glycitein, and genistein were increased. As for prenylated flavonoids from C. tricuspidata, these metabolites were decreased at the early stage of fermentation, and were increased at end of the fermentation. In terms of the functional food product, various metabolites derived from diverse natural products in CLM had complementary effects and demonstrated higher antioxidant and pancreatic lipase inhibition activities by fermentation; these activities were closely related to flavonoid aglycones including genistein, daidzein, glycitein, luteolin, and quercetin. In vivo experiment, several clinical parameters affected by HFD were remarkably improved by the administration of either CLM or FCLM, but there was a difference in the antiobesity effects. The levels of lysoPCs with C20:4, C16:0, and C22:6 were significantly attenuated by CLM administration, while the attenuated levels of lysoPCs with C20:4 and C18:2 were significantly restored by FCLM administration. These metabolites may explain the above-mentioned differences in antiobesity effects. Although only the changes in plasma lysophospholipids could not fully explain antiobesity effects between non-fermented and fermented plant mixtures from our results, we suggest that metabolomics approach could provide a way to reveal the metabolite alterations in the complex fermentation process and understand the differences or changes in bioactivity according to fermentation.
Institute
Konkuk university
Last NameSuh
First NameDong Ho
AddressNeong-Dong-ro 120, Seoul, Kwang-Gin-gu, 05029, Korea, South
Emailsdh14031988@naver.com
Phone82-02-444-4290
Submit Date2016-01-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2016-01-20
Release Version1
Dong Ho Suh Dong Ho Suh
https://dx.doi.org/10.21228/M8TG7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000255
Project DOI:doi: 10.21228/M8TG7T
Project Title:Comparison of Metabolites Variation and Antiobesity Effects of a Mixture of Cudrania tricuspidata, Lonicera caerulea, and the Soybean According to Fermentation in vitro and in vivo
Project Summary:We used ultra-performance-liquid-chromatography with quadrupole-time-of-flight mass spectrometry to study the changes in metabolites in the mixture of Cudrania tricuspidata, Lonicera caerulea, and soybean (CLM) during fermentation. Additionally, the antiobesity effects of CLM and fermented-CLM (FCLM) were studied based on the analysis of plasma from high-fat diet (HFD)-fed mice. The levels of cyanidin and the glycosides of luteolin, quercetin, and cyanidin derived from L. caerulea were decreased, whereas the levels of luteolin and quercetin were increased during fermentation. Isoflavone glycosides and soyasaponins originating from the soybean were decreased, whereas their aglycones such as daidzein, glycitein, and genistein were increased. As for prenylated flavonoids from C. tricuspidata, these metabolites were decreased at the early stage of fermentation, and were increased at end of the fermentation. In terms of the functional food product, various metabolites derived from diverse natural products in CLM had complementary effects and demonstrated higher antioxidant and pancreatic lipase inhibition activities by fermentation; these activities were closely related to flavonoid aglycones including genistein, daidzein, glycitein, luteolin, and quercetin. In vivo experiment, several clinical parameters affected by HFD were remarkably improved by the administration of either CLM or FCLM, but there was a difference in the antiobesity effects. The levels of lysoPCs with C20:4, C16:0, and C22:6 were significantly attenuated by CLM administration, while the attenuated levels of lysoPCs with C20:4 and C18:2 were significantly restored by FCLM administration. These metabolites may explain the above-mentioned differences in antiobesity effects. Although only the changes in plasma lysophospholipids could not fully explain antiobesity effects between non-fermented and fermented plant mixtures from our results, we suggest that metabolomics approach could provide a way to reveal the metabolite alterations in the complex fermentation process and understand the differences or changes in bioactivity according to fermentation.
Institute:Konkuk university
Last Name:Suh
First Name:Dong Ho
Address:Neong-Dong-ro 120, Seoul, Kwang-Gin-gu, 05029, Korea, South
Email:sdh14031988@naver.com
Phone:82-02-444-4290

Subject:

Subject ID:SU000336
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA014424HCLM7HCLM
SA014425HCLM10HCLM
SA014426HCLM9HCLM
SA014427HCLM5HCLM
SA014428HCLM8HCLM
SA014429HCLM6HCLM
SA014430HCLM1HCLM
SA014431HCLM3HCLM
SA014432HCLM2HCLM
SA014433HCLM4HCLM
SA014434HD8HD
SA014435HD9HD
SA014436HD10HD
SA014437HD7HD
SA014438HD5HD
SA014439HD1HD
SA014440HD6HD
SA014441HD3HD
SA014442HD2HD
SA014443HD4HD
SA014444HFCLM5HFCLM
SA014445HFCLM6HFCLM
SA014446HFCLM7HFCLM
SA014447HFCLM4HFCLM
SA014448HFCLM1HFCLM
SA014449HFCLM3HFCLM
SA014450HFCLM2HFCLM
SA014451HX10HX
SA014452HX4HX
SA014453HX3HX
SA014454HX2HX
SA014455HX9HX
SA014456HX5HX
SA014457HX1HX
SA014458HX7HX
SA014459HX8HX
SA014460HX6HX
SA014461ND5ND
SA014462ND4ND
SA014463ND2ND
SA014464ND6ND
SA014465ND3ND
SA014466ND8ND
SA014467ND10ND
SA014468ND9ND
SA014469ND1ND
SA014470ND7ND
Showing results 1 to 47 of 47

Collection:

Collection ID:CO000330
Collection Summary:Plasma 200uL were extracted with 1mL of 80% MeOH
Sample Type:Blood

Treatment:

Treatment ID:TR000350
Treatment Summary:Seven-week-old C57BL6J male mice were purchased from Daehan bio-link (Chungbuk, Republic of Korea). All mice were acclimated for 1 week under controlled conditions (temperature: 25 ± 2°C, relative humidity: 50 ± 5%, and 12 h light/dark cycle). The animals had free access to a normal diet (AIN-76A, Research Diets, Inc., NJ, USA) and water. After 1 week, the mice were randomly distributed into 5 groups: (1) group normal-diet (ND) (n = 11) was fed a normal diet for 6 weeks, (2) group HFD (named HD; n = 10) was fed 60 kcal% fat (D1242, Research Diets, Inc., NJ, USA) for 6 weeks, (3) group HFD with xenical administration (HX, 50 mg?kg?1?day?1) n = 10, 6 weeks, (4) group HFD with CLM administration (HCLM, 2 g?kg?1?day?1) n = 10, 6 weeks; and (5) group HFD with FCLM (CLM fermented for 60 h) administration (HFCLM, 2 g?kg?1?day?1) n = 8, 6 weeks. In this study, we used xenical as a positive control for antiobesity effects. Xenical, CLM, and FCLM dissolved with saline were orally administered everyday into the stomach with an oral zonde needle. And, the equal volume of saline were applied for ND and HD groups. During the experimental periods, the feed intake and the body weight of the mice were measured daily.
Treatment Protocol Filename:DongHo_20160115_025706_PR_CO_The_animal_experiment.docx

Sample Preparation:

Sampleprep ID:SP000344
Sampleprep Summary:For blood collection, the mice were sacrificed by cardiac puncture. Plasma (100 µL) was subjected to extraction with cold methanol (500 µL) on a MM400 mixer mill (Retsch®, Haan, Germany) with the frequency 30 s?1 for 5 min. The suspension was centrifuged at 12,578 g for 10 min at 4°C. The supernatant was filtered through a 0.2-µm PTEE filter and evaporated in a speed vacuum concentrator. The final concentration of the plasma was 5 mg/mL in methanol for the UPLC-Q-TOF-MS analysis.

Combined analysis:

Analysis ID AN000502
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Micromass QTOF Premier
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000354
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000438
Analysis ID:AN000502
Instrument Name:Waters Micromass QTOF Premier
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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