Summary of study ST000335

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000267. The data can be accessed directly via it's Project DOI: 10.21228/M88K54 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000335
Study TitleMetabolomics of bovine uterine fluid at the onset of conceptus elongation
Study TypeProspective cohort study
Study SummaryThe objective is to investigate changes in metabolomics of uterine lumen content of lactating dairy cows associated with the onset of conceptus (embryo and associated membranes) elongation. Lactating dairy cows had estrous cycles synchronized and were subjected to induced ovulation and timed artificial insemination (AI). The day of AI was considered study d 0. On d 15, uteri were flushed by transcervical catheterization and infusion of 20 mL of phosphate buffered solution with 0.1% of polyvinyl acetate. Recovered conceptuses were classified based on morphology/length as ovoid (OV; 1-4 mm), tubular (TUB; 5-19 mm) and filamentous (FIL; 20-85 mm). The first 20 mL infused in the uterus were recovered, placed in conical tubes and centrifuged at 2,000 × g at 4ᵒC. The supernatant was collect, aliquoted and stored at -80ᵒC for later analyses of fluid composition, including measurement of IFN-τ concentration. Cows with no conceptus recovered and no detection of IFN-τ in uterine flushing were considered as nonpregnant (NPREG). The experimental design was then considered a prospective cohort study with 4 independent groups (NPREG, OV, TUB, and FIL). The additional 5th group represents a specific physiological condition of cows within the study and it will be compared to TUB and FIL groups combined, working as a pilot study for future research.
Institute
University of Florida
DepartmentSECIM
LaboratoryLaboratory of Reproduction and Nutrition of Dairy Cows
Last NameRibeiro
First NameEduardo
Address2250 Shealy Drive, Bldg. 499, Room 228, Gainesville, FL 32611-0910
Emailribeiro.es@ufl.edu
Phone3522227333
Submit Date2016-01-17
Num Groups5
Total Subjects30
Analysis Type DetailLC-MS
Release Date2017-01-17
Release Version1
Eduardo Ribeiro Eduardo Ribeiro
https://dx.doi.org/10.21228/M88K54
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000267
Project DOI:doi: 10.21228/M88K54
Project Title:Metabolomics of bovine uterine fluid at the onset of conceptus elongation
Project Type:Research
Project Summary:Research The objective is to investigate changes in metabolomics of uterine lumen content of lactating dairy cows associated with the onset of conceptus (embryo and associated membranes) elongation. Lactating dairy cows had estrous cycles synchronized and were subjected to induced ovulation and timed artificial insemination (AI). The day of AI was considered study d 0. On d 15, uteri were flushed by transcervical catheterization and infusion of 20 mL of phosphate buffered solution with 0.1% of polyvinyl acetate. Recovered conceptuses were classified based on morphology/length as ovoid (OV; 1-4 mm), tubular (TUB; 5-19 mm) and filamentous (FIL; 20-85 mm). The first 20 mL infused in the uterus were recovered, placed in conical tubes and centrifuged at 2,000 × g at 4ᵒC. The supernatant was collect, aliquoted and stored at -80ᵒC for later analyses of fluid composition, including measurement of IFN-τ concentration. Cows with no conceptus recovered and no detection of IFN-τ in uterine flushing were considered as nonpregnant (NPREG). The experimental design was then considered a prospective cohort study with 4 independent groups (NPREG, OV, TUB, and FIL). The additional 5th group represents a specific physiological condition of cows within the study and it will be compared to TUB and FIL groups combined, working as a pilot study for future research.
Institute:University of Florida
Department:Department of Animal Sciences
Laboratory:Laboratory of Reproduction and Nutrition of Dairy Cows
Last Name:Santos
First Name:José Eduardo
Address:2250 Shealy Drive, Bldg. 499, Room 204, Gainesville, FL 32611-0910
Email:jepsantos@ufl.edu
Phone:3522227333
Funding Source:Internal
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