Summary of Study ST000346
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000278. The data can be accessed directly via it's Project DOI: 10.21228/M8030X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000346 |
Study Title | Metabolites detected from human bronchoalveolar lavage |
Study Summary | This is a preliminary trial to determine how viable this system will be to use on a much larger number of samples (up to 150). We would like to determine the range, number of metabolite species, and relative concentrations than can be detected in human bronchoalveolar lavage. We would also like to determine how clear the distinction is between the 3 patient groups as this will inform us as to how many of the 150 samples we need to run in the study proper. |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2016-02-11 |
Num Groups | 8 |
Total Subjects | 32 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-03-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000278 |
Project DOI: | doi: 10.21228/M8030X |
Project Title: | Metabolites detected from human bronchoalveolar lavage |
Project Summary: | This is a preliminary trial to determine how viable this system will be to use on a much larger number of samples (up to 150). We would like to determine the range, number of metabolite species, and relative concentrations than can be detected in human bronchoalveolar lavage. We would also like to determine how clear the distinction is between the 3 patient groups as this will inform us as to how many of the 150 samples we need to run in the study proper. |
Institute: | University of California, Davis |
Last Name: | Fiehn |
First Name: | Oliver |
Address: | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
Email: | ofiehn@ucdavis.edu |
Phone: | (530) 754-8258 |
Funding Source: | NIH U24DK097154 |
Subject:
Subject ID: | SU000366 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Condition |
---|---|---|
SA015294 | BAL_Mild_5148_009 | Mild Asthma |
SA015295 | BAL_Mild_7693_006 | Mild Asthma |
SA015296 | BAL_Mild_7223_007 | Mild Asthma |
SA015297 | BAL_Mild_5965_010 | Mild Asthma |
SA015298 | BAL_Mild_6816_008 | Mild Asthma |
SA015299 | BAL_Normal_7230_013 | No Asthma |
SA015300 | BAL_Normal_7376_011 | No Asthma |
SA015301 | BAL_Normal_7042_014 | No Asthma |
SA015302 | BAL_Normal_7295_012 | No Asthma |
SA015303 | BAL_Normal_6880_015 | No Asthma |
SA015304 | Kailko_Biorec01 | Quality Control |
SA015305 | Kailko_Biorec01_1 | Quality Control |
SA015306 | BAL_Severe_7747_007 | Severe Asthma |
SA015307 | BAL_Severe_7277_002 | Severe Asthma |
SA015308 | BAL_Severe_5842_005 | Severe Asthma |
SA015309 | BAL_Severe_6247_004 | Severe Asthma |
SA015310 | BAL_Severe_6339_003 | Severe Asthma |
SA015311 | BAL_Severe_7747_001 | Severe Asthma |
Showing results 1 to 18 of 18 |
Collection:
Collection ID: | CO000360 |
Collection Summary: | 50ml of hospital grade sterile saline was instilled into the lungs of human volunteers. As much liquid as possible is then gently suctioned out. The sample is then centrifuged to remove cellular material and debris, and supernatant collected and frozen at -80C |
Collection Protocol Filename: | StudyDesign-GerardKaiko10202015.pdf |
Sample Type: | Bronchoalveolar lavage supernatant |
Treatment:
Treatment ID: | TR000380 |
Treatment Summary: | The 3 groups are as follows: 1. Healthy controls (5) 2. Subjects with mild asthma (5) 3. Subjects with severe asthma (5) |
Treatment Protocol Filename: | StudyDesign-GerardKaiko10202015.pdf |
Sample Preparation:
Sampleprep ID: | SP000373 |
Sampleprep Summary: | None |
Combined analysis:
Analysis ID | AN000561 | AN000562 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 6530 | Agilent 6550 |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000398 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
Instrument Name: | Agilent 6530 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 450-850 bar |
Column Temperature: | 65 C |
Flow Gradient: | 15% B to 99% B |
Flow Rate: | 0.6 mL/min |
Sample Injection: | 1.67 uL |
Solvent A: | 60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate |
Analytical Time: | 13 min |
Capillary Voltage: | 3500 |
Time Program: | 15 min |
Weak Wash Solvent Name: | Isopropanol |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | Same |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000399 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 450-850 bar |
Column Temperature: | 65 C |
Flow Gradient: | 15% B to 99% B |
Flow Rate: | 0.6 mL/min |
Sample Injection: | 5.0 uL |
Solvent A: | 60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate |
Analytical Time: | 13 min |
Capillary Voltage: | 3500 |
Time Program: | 15 min |
Weak Wash Solvent Name: | Isopropanol |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | Same |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000497 |
Analysis ID: | AN000561 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs Lipidomics runs are performed in negative and positve mode (two filenames) |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 200 |
Ion Spray Voltage: | 1000 |
Ionization: | Neg |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Dataformat: | .d |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |
MS ID: | MS000498 |
Analysis ID: | AN000562 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs Lipidomics runs are performed in negative and positve mode (two filenames) |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 13 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 175 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 200 |
Ion Spray Voltage: | 1000 |
Ionization: | Neg |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Dataformat: | .d |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |