Summary of Study ST000354
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000283. The data can be accessed directly via it's Project DOI: 10.21228/M8BC8T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000354 |
Study Title | Metabolite comparison of mouse gastric tissue and glands |
Study Summary | The goal of this project was to compare the metabolite profiles of the: mouse gastric antrum and the mouse gastric corpus, the mouse gastric antrum and the mouse gastric antrum isolated glands, and the mouse gastric corpus and the mouse gastric corpus isolated glands. |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2016-02-09 |
Num Groups | 8 |
Total Subjects | 32 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-03-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000283 |
Project DOI: | doi: 10.21228/M8BC8T |
Project Title: | Metabolite comparison of mouse gastric tissue and glands |
Project Summary: | The goal of this project was to compare the metabolite profiles of the: mouse gastric antrum and the mouse gastric corpus, the mouse gastric antrum and the mouse gastric antrum isolated glands, and the mouse gastric corpus and the mouse gastric corpus isolated glands. |
Institute: | University of California, Davis |
Department: | Genome and Biomedical Sciences Facility |
Laboratory: | WCMC Metabolomics Core |
Last Name: | Fiehn |
First Name: | Oliver |
Address: | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
Email: | ofiehn@ucdavis.edu |
Phone: | (530) 754-8258 |
Funding Source: | NIH U24DK097154 |
Subject:
Subject ID: | SU000374 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Organ |
---|---|---|
SA015493 | 8 | Antrum |
SA015494 | 10 | Antrum |
SA015495 | 12 | Antrum |
SA015496 | 7 | Antrum |
SA015497 | 11 | Antrum |
SA015498 | 9 | Antrum |
SA015499 | 15 | Antrum Glands |
SA015500 | 16 | Antrum Glands |
SA015501 | 6 | Corpus |
SA015502 | 5 | Corpus |
SA015503 | 1 | Corpus |
SA015504 | 2 | Corpus |
SA015505 | 4 | Corpus |
SA015506 | 3 | Corpus |
SA015507 | 14 | Corpus Glands |
SA015508 | 13 | Corpus Glands |
SA015509 | Pool04 | Plasma |
SA015510 | Biorec02 | Plasma |
SA015511 | Pool03 | Plasma |
SA015512 | Pool02 | Plasma |
SA015513 | Biorec01 | Plasma |
SA015514 | Pool01 | Plasma |
SA015515 | Biorec03 | Plasma |
Showing results 1 to 23 of 23 |
Collection:
Collection ID: | CO000368 |
Collection Summary: | The gastric samples (A and C) were prepared by dissecting tissue from the mouse, rinsing in PBS, and flash freezing on liquid N2. The samples were stored at 80 C. The glands samples (Aglands and Cglands) by isolating gastric glands from mouse stomach tissue using EDTA and Sucrose and sorbitol. The glands were centrifuged and the supernatant removed, but there is probably still sucrose and sorbitol left. These were flash frozen on liquid N2 and stored at 80 C. |
Collection Protocol Filename: | StudyDesign-KarenOttemann-81215-1.pdf |
Sample Type: | Tissue |
Treatment:
Treatment ID: | TR000388 |
Treatment Summary: | A. Mouse gastric antrum (n=6) C. Mouse gastric corpus (n=6) Aglands. Mouse gastric antrum isolated glands (n=2) Cglands. Mouse gastric corpus isolated glands (n=2) |
Treatment Protocol Filename: | StudyDesign-KarenOttemann-81215-1.pdf |
Sample Preparation:
Sampleprep ID: | SP000381 |
Sampleprep Summary: | None |
Combined analysis:
Analysis ID | AN000577 | AN000578 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 6530 | Agilent 6550 |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000412 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
Instrument Name: | Agilent 6530 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 450-850 bar |
Column Temperature: | 65 C |
Flow Gradient: | 15% B to 99% B |
Flow Rate: | 0.6 mL/min |
Internal Standard: | See data dictionary |
Retention Time: | See data dictionary |
Sample Injection: | 1.67 uL |
Solvent A: | 60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate |
Analytical Time: | 13 min |
Capillary Voltage: | 3500 |
Time Program: | 15 min |
Weak Wash Solvent Name: | Isopropanol |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | Same |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000413 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 450-850 bar |
Column Temperature: | 65 C |
Flow Gradient: | 15% B to 99% B |
Flow Rate: | 0.6 mL/min |
Internal Standard: | See data dictionary |
Retention Time: | See data dictionary |
Sample Injection: | 5.0 uL |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 10mM acetic acid; 10mM ammonium acetate |
Analytical Time: | 13 min |
Capillary Voltage: | 3500 |
Time Program: | 15 min |
Weak Wash Solvent Name: | Isopropanol |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | Same |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000513 |
Analysis ID: | AN000577 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 |
Ion Spray Voltage: | 1000 |
Ionization: | Pos |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Dataformat: | .d |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |
MS ID: | MS000514 |
Analysis ID: | AN000578 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are performed in negative and positve mode (two filenames) |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 13 l/min |
Dry Gas Temp: | 200 |
Fragment Voltage: | 175 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 |
Ion Spray Voltage: | 1000 |
Ionization: | Neg |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Dataformat: | .d |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |