Summary of Study ST000361

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000287. The data can be accessed directly via it's Project DOI: 10.21228/M8Z310 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000361
Study TitleCharacterization and Plasticity of the Metabolome in Peripheral Cells in Bipolar I Disorder
Study TypeBroad Spectrum LCMS
Study SummaryPatients with Bipolar I Disorder (BPI) and matched non-affected controls were recruited. Males and females from all races and ethnicities between the ages of 19-65 participated in the research. Skin biopsies were obtained and fibroblasts were isolated from each of these biopsies. A total of 1 x 〖10〗^7 fibroblasts cells were used for metabolomics. Cell pellets were flash frozen in liquid nitrogen and shipped on dry ice to the NIH Eastern Regional Comprehensive Metabolomic Resource Core at RTI International in North Carolina. Metabolomics will be determined using a broad spectrum metabolomics protocol by RTI
Institute
University of North Carolina
DepartmentDiscovery Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-03-05
Num Groups2
Total Subjects18
Num Males7
Num Females11
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2017-03-03
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8Z310
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000287
Project DOI:doi: 10.21228/M8Z310
Project Title:Characterization and Plasticity of the Metabolome in Peripheral Cells in Bipolar I Disorder
Project Type:Broad Spectrum LCMS
Project Summary:Bipolar disorder is a serious condition characterized by severe and debilitating elevations and depressions in mood and energy (1). Bipolar I disorder (BPI) is a particularly severe phenotype which is generally defined as having periods of abnormal moods ranging from “highs,” (mania or hypomania), to “lows” (depression), alternating with periods of relatively normal moods. Key problems in treating bipolar disorder relate to the fact that the pathophysiology of the disorder remains elusive; as a result, there are currently no laboratory tests for diagnosis, to aid treatment decisions, or to predict treatment responses. Recent advances in the study of bipolar disorder increasingly implicate mitochondrial and bioenergetic dysfunction as a common feature of the pathophysiology of the disorder (2). Shifts in metabolism from mitochondrial oxidative phosphorylation toward glycolysis have been observed in the brains of bipolar patients experiencing depressive mood states (3). Increased oxidative stress has also been shown in postmortem brain from bipolar patients (4), and in cultured peripheral fibroblasts from patients with major depression (5). However, little is known about the inherent metabolic state of peripheral cells during bipolar disorder, which may reflect the overall metabolic profile of the individual and may also impact the supply of nutrients to the brain. We propose to characterize the baseline metabolomic profiles of peripheral cells in cultured fibroblasts from patients with BPI and matched non-affected controls. Patients with Bipolar I Disorder (BPI) and matched non-affected controls were recruited. Males and females from all races and ethnicities between the ages of 19-65 participated in the research. Skin biopsies were obtained and fibroblasts were isolated from each of these biopsies. A total of 1 x 〖10〗^7 fibroblasts cells were used for metabolomics. Cell pellets were flash frozen in liquid nitrogen and shipped on dry ice to the NIH Eastern Regional Comprehensive Metabolomic Resource Core at RTI International in North Carolina. Metabolomics will be determined using a broad spectrum metabolomics protocol by RTI.
Institute:University of Alabama, Birmingham
Department:School of Medicine
Laboratory:Department of Pathology
Last Name:Landar
First Name:Aimee
Address:BMR2 506 901 9th St South, Birmingham AL 35205
Email:landar@uab.edu
Phone:205-975-9507
Funding Source:NIH, Common Fund, Pilot and Feasibility

Subject:

Subject ID:SU000382
Subject Type:human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:27-61 years
Gender:male/female
Human Race:caucasian, hispanic, african american
Species Group:Human

Factors:

Subject type: human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA016011Total_Pool_02-
SA016012Total_Pool_01-
SA016013Total_Pool_04-
SA016014Total_Pool_03-
SA016015Total_Pool_05-
SA016016A_0131Bipolar
SA016017A_0276Bipolar
SA016018A_0293Bipolar
SA016019A_0110Bipolar
SA016020A_0245Bipolar
SA016021A_0191Bipolar
SA016022A_0302Bipolar
SA016023B_0312Control
SA016024B_0128Control
SA016025B_0247Control
SA016026B_0061Control
SA016027B_0255Control
SA016028B_0318Control
SA016029B_0183Control
SA016030B_0203Control
SA016031B_0107Control
SA016032B_0182Control
SA016033B_0151Control
Showing results 1 to 23 of 23

Collection:

Collection ID:CO000376
Collection Summary:For extraction, 500 µL of an ice-cold solution of 0.005 mg/mL Tryptohan-d5 in 90:10 Methanol:Chloroform (v/v) was added to the tubes containing the thawed human dermal fibroblasts cell pellets. MagNA Lyser ceramic beads were added to the tubes, and a MagNA Lyser was used to beat the samples for two thirty seconds pulses at 2,000 rpm, placing the samples on cold block for 5 minutes in between pulses. Sonication for five minutes was performed to the beaten cell samples, followed by centrifugation at room temperature and at 16,000 rcf for 4 minutes. A 300 µL aliquot of each experimental cell sample homogenized supernatant was transferred to new pre-labeled 2.0 mL LoBind Eppendorf tubes and stored at -80 °C for one hour.
Sample Type:fibroblasts

Treatment:

Treatment ID:TR000396
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP000389
Sampleprep Summary:Whole study pooled QC samples were created by combining 175 µL aliquot from each of the study samples into a 20 mL scintillation vial. The QC pooled sample was then vortexed for 30 seconds on an analog vortex mixer. Aliquots, 300 µL, were transferred to five new pre-labeled 2.0 mL LoBind Eppendorf tubes and stored at -80 °C for one hour. Next, the supernatants of the study and whole study pooled QC samples, generated above, were dried overnight using a lyophilizer. The residue was reconstituted in 75 µL of reconstitution buffer ( 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for five minutes at 5,000 rpm. Then, the samples were centrifuged at room temperature at 16,000 rcf for four minutes, and the supernatants were transferred to clean pre-labeled autosampler vials for data acquisition.

Combined analysis:

Analysis ID AN000592 AN000593
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters ACQUITY UPLC HSS T3 Waters ACQUITY UPLC HSS T3
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 Si QTOF Waters Synapt G2 Si QTOF
Ion Mode POSITIVE NEGATIVE
Units intensity intensity

Chromatography:

Chromatography ID:CH000424
Chromatography Summary:UPLC-MS spectra were collected for all samples. UPLC was performed on a Waters Acquity UPLC with an Acquity HSS T3 column (2.1x 100mm x 1.8 µm) at 50 °C using the reversed-phase separation. Water with 0.1% formic acid (mobile phase A) and methanol with 0.1% formic acid (mobile phase B) were used as mobile phases and the metabolites were chromatographically separated using a gradient separation: (see the 3. Sheridan-Mice-Microglia Cells_RP-Metadata and Analytical Metadata.xlsx file for the flow gradient). Mass spectroscopy analysis was performed using a Synapt G2 Si ESI-Q-TOF using a 10 µL injection volume. UPLC-MS data were collected over 70-1000 m/z in both positive and negative modes.
Methods Filename:RTI-RCMRC-RP
Instrument Name:Waters Acquity
Column Name:Waters ACQUITY UPLC HSS T3
Flow Rate:.4ml/min
Injection Temperature:8 C
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS000528
Analysis ID:AN000592
Instrument Name:Waters Synapt G2 Si QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:RTI-RCMRC-RP-POS
  
MS ID:MS000529
Analysis ID:AN000593
Instrument Name:Waters Synapt G2 Si QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:RTI-RCMRC-RP-NEG
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