Summary of Study ST000370
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000370 |
| Study Title | Primary T Cell Noxa Knockdown (Donor 8) III |
| Study Type | Regular |
| Study Summary | Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. For this part of the experiment, the spent medium was collected for analysis. All samples were immediately stored in -80oC. |
| Institute | University of Michigan |
| Department | Biomedical Research |
| Laboratory | Core Facilities Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2015-03-27 |
| Num Groups | 3 |
| Total Subjects | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000129 |
| Project DOI: | doi: 10.21228/M85C7W |
| Project Title: | Metabolic effects of Noxa |
| Project Summary: | labeled glutamine metabolomics on Noxa expressing cells |
| Institute: | University of Minnesota |
| Department: | Masonic Cancer Center |
| Laboratory: | Kelekar Lab |
| Last Name: | Kelekar |
| First Name: | Ameeta |
| Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
| Email: | ameeta@umn.edu |
| Phone: | 612-626-2358 |
Subject:
| Subject ID: | SU000391 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Glucose label | Glutamine label |
|---|---|---|---|
| SA016894 | S00017978 | no | no |
| SA016895 | S00017970 | no | no |
| SA016896 | S00017971 | no | yes |
| SA016897 | S00017964 | no | yes |
| SA016898 | S00017969 | no | yes |
| SA016899 | S00017965 | no | yes |
| SA016900 | S00017966 | no | yes |
| SA016901 | S00017967 | no | yes |
| SA016902 | S00017968 | no | yes |
| SA016903 | S00017979 | yes | no |
| SA016904 | S00017977 | yes | no |
| SA016905 | S00017972 | yes | no |
| SA016906 | S00017973 | yes | no |
| SA016907 | S00017974 | yes | no |
| SA016908 | S00017975 | yes | no |
| SA016909 | S00017976 | yes | no |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000385 |
| Collection Summary: | None |
| Sample Type: | Culture Media |
Treatment:
| Treatment ID: | TR000405 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000398 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Fluxomics analysis protocol-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000604 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000430 |
| Methods Filename: | DQM0208 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000537 |
| Analysis ID: | AN000604 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
