Summary of Study ST000372

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000372
Study TitleMeasure change in metabolites based on diet and feeding status (part I)
Study TypeRegular
Study SummaryFlies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-18
Num Groups4
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HP4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000134
Project DOI:doi: 10.21228/M8HP4Z
Project Title:Fly Brain Studies
Project Summary:Measure change in metabolites according to diet and feeding state
Institute:University of Michigan
Department:MCDB LS&A
Laboratory:Dus Lab
Last Name:Dus
First Name:Monica
Address:Ann Arbor, MI
Email:mdus@umich.edu
Phone:347-891-2287

Subject:

Subject ID:SU000393
Subject Type:Animal
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Species Group:Insect

Factors:

Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id State Diet
SA016950S00018443sated high sugar
SA016951S00018447sated high sugar
SA016952S00018446sated high sugar
SA016953S00018444sated high sugar
SA016954S00018445sated high sugar
SA016955S00018451sated normal
SA016956S00018449sated normal
SA016957S00018450sated normal
SA016958S00018448sated normal
SA016959S00018458starved high sugar
SA016960S00018459starved high sugar
SA016961S00018460starved high sugar
SA016962S00018457starved high sugar
SA016963S00018452starved normal
SA016964S00018453starved normal
SA016965S00018454starved normal
SA016966S00018455starved normal
SA016967S00018456starved normal
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000387
Collection Summary:none
Sample Type:Tissues

Treatment:

Treatment ID:TR000407
Treatment Summary:none
Treatment Protocol Filename:Acyl-CoA_-20150305.docx

Sample Preparation:

Sampleprep ID:SP000400
Sampleprep Summary:none
Sampleprep Protocol Filename:Acyl-CoA_-20150305.docx

Combined analysis:

Analysis ID AN000606
Analysis type MS
Chromatography type Reversed phase
Chromatography system None
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode POSITIVE
Units pmol/µg tissue protein

Chromatography:

Chromatography ID:CH000432
Methods ID:DQM0127
Methods Filename:QM-005-Xbrdg2mm-CoA-6490.m.zip
Instrument Name:None
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000539
Analysis ID:AN000606
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:QM-005-Xbrdg2mm-CoA-6490.m.zip
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