Summary of Study ST000373

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000373
Study TitleMeasure change in metabolites based on diet and feeding status (part II)
Study TypeRegular
Study SummaryFlies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates
Institute
University of Michigan
DepartmentBiomedical Research Core
LaboratoryFacilities Metabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-20
Num Groups4
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HP4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000134
Project DOI:doi: 10.21228/M8HP4Z
Project Title:Fly Brain Studies
Project Summary:Measure change in metabolites according to diet and feeding state
Institute:University of Michigan
Department:MCDB LS&A
Laboratory:Dus Lab
Last Name:Dus
First Name:Monica
Address:Ann Arbor, MI
Email:mdus@umich.edu
Phone:347-891-2287

Subject:

Subject ID:SU000394
Subject Type:Animal
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Species Group:Insect

Factors:

Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id State Diet
SA016968S00018443sated high sugar
SA016969S00018447sated high sugar
SA016970S00018446sated high sugar
SA016971S00018444sated high sugar
SA016972S00018445sated high sugar
SA016973S00018451sated normal
SA016974S00018449sated normal
SA016975S00018450sated normal
SA016976S00018448sated normal
SA016977S00018458starved high sugar
SA016978S00018459starved high sugar
SA016979S00018460starved high sugar
SA016980S00018457starved high sugar
SA016981S00018452starved normal
SA016982S00018453starved normal
SA016983S00018454starved normal
SA016984S00018455starved normal
SA016985S00018456starved normal
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000388
Collection Summary:NONE
Sample Type:Tissues

Treatment:

Treatment ID:TR000408
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000401
Sampleprep Summary:none
Sampleprep Protocol Filename:Glycolysis-TCA-nucleotides-20150324.docx

Combined analysis:

Analysis ID AN000607
Analysis type MS
Chromatography type HILIC
Chromatography system Unspecified
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units pmol/µg tissue protein

Chromatography:

Chromatography ID:CH000433
Methods ID:DQM0108
Methods Filename:GlyTCA-ESN-HILIC-6490.m.zip
Instrument Name:Unspecified
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000540
Analysis ID:AN000607
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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