Summary of Study ST000373
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000373 |
Study Title | Measure change in metabolites based on diet and feeding status (part II) |
Study Type | Regular |
Study Summary | Flies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates |
Institute | University of Michigan |
Department | Biomedical Research Core |
Laboratory | Facilities Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | Ann Arbor, MI |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2016-04-20 |
Num Groups | 4 |
Total Subjects | 18 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000134 |
Project DOI: | doi: 10.21228/M8HP4Z |
Project Title: | Fly Brain Studies |
Project Summary: | Measure change in metabolites according to diet and feeding state |
Institute: | University of Michigan |
Department: | MCDB LS&A |
Laboratory: | Dus Lab |
Last Name: | Dus |
First Name: | Monica |
Address: | Ann Arbor, MI |
Email: | mdus@umich.edu |
Phone: | 347-891-2287 |
Subject:
Subject ID: | SU000394 |
Subject Type: | Animal |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Species Group: | Insects |
Factors:
Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)
mb_sample_id | local_sample_id | State | Diet |
---|---|---|---|
SA016968 | S00018443 | sated | high sugar |
SA016969 | S00018447 | sated | high sugar |
SA016970 | S00018446 | sated | high sugar |
SA016971 | S00018444 | sated | high sugar |
SA016972 | S00018445 | sated | high sugar |
SA016973 | S00018451 | sated | normal |
SA016974 | S00018449 | sated | normal |
SA016975 | S00018450 | sated | normal |
SA016976 | S00018448 | sated | normal |
SA016977 | S00018458 | starved | high sugar |
SA016978 | S00018459 | starved | high sugar |
SA016979 | S00018460 | starved | high sugar |
SA016980 | S00018457 | starved | high sugar |
SA016981 | S00018452 | starved | normal |
SA016982 | S00018453 | starved | normal |
SA016983 | S00018454 | starved | normal |
SA016984 | S00018455 | starved | normal |
SA016985 | S00018456 | starved | normal |
Showing results 1 to 18 of 18 |
Collection:
Collection ID: | CO000388 |
Collection Summary: | NONE |
Sample Type: | Tissues |
Treatment:
Treatment ID: | TR000408 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000401 |
Sampleprep Summary: | none |
Sampleprep Protocol Filename: | Glycolysis-TCA-nucleotides-20150324.docx |
Combined analysis:
Analysis ID | AN000607 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Unspecified |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410A QQQ |
Ion Mode | NEGATIVE |
Units | pmol/µg tissue protein |
Chromatography:
Chromatography ID: | CH000433 |
Methods ID: | DQM0108 |
Methods Filename: | GlyTCA-ESN-HILIC-6490.m.zip |
Instrument Name: | Unspecified |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS000540 |
Analysis ID: | AN000607 |
Instrument Name: | Agilent 6410A QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |