Summary of Study ST000375
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000295. The data can be accessed directly via it's Project DOI: 10.21228/M8X60B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000375 |
| Study Title | Mice exercise metabolomics (part II) |
| Study Type | Regular |
| Study Summary | Mice were run for 15 minutes on a treadmill and then mice were induced into anesthesia at a dose of 5% isoflurane, then maintained by continuous inhalation of 2% isoflurane. Quadriceps muscle was collected first and flash frozen in liquid nitrogen. Then blood was collected from the left ventricle of the heart and placed into serum separating tubes. Blood was allowed to clot for 40 minutes and centrifuged, the supernatant was frozen in liquid nitrogen |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2016-04-21 |
| Num Groups | 4 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000295 |
| Project DOI: | doi: 10.21228/M8X60B |
| Project Title: | Metabolomics Mice Studies |
| Project Summary: | Analysis of glycolysis/TCA cycle in skeletal muscle + acyl-carnitine in serum of wt and 113Q mice |
| Institute: | University of Michigan |
| Department: | Pathology |
| Laboratory: | Lieberman, Andrew |
| Last Name: | Lieberman |
| First Name: | Andrew |
| Address: | Ann Arbor, MI |
| Email: | liebermn@umich.edu |
| Phone: | 734-936-1889 |
Subject:
| Subject ID: | SU000396 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Wild Type | Mutants |
|---|---|---|---|
| SA017002 | S00018468 | no | yes |
| SA017003 | S00018474 | no | yes |
| SA017004 | S00018473 | no | yes |
| SA017005 | S00018467 | no | yes |
| SA017006 | S00018465 | no | yes |
| SA017007 | S00018475 | no | yes |
| SA017008 | S00018466 | no | yes |
| SA017009 | S00018476 | no | yes |
| SA017010 | S00018472 | yes | no |
| SA017011 | S00018469 | yes | no |
| SA017012 | S00018463 | yes | no |
| SA017013 | S00018462 | yes | no |
| SA017014 | S00018464 | yes | no |
| SA017015 | S00018461 | yes | no |
| SA017016 | S00018470 | yes | no |
| SA017017 | S00018471 | yes | no |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000390 |
| Collection Summary: | NONE |
| Sample Type: | Serum |
Treatment:
| Treatment ID: | TR000410 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000403 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides analysis protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000609 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | QTOF-002-HILIC-35min-1mm-No Insert.m.zip |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410A QQQ |
| Ion Mode | NEGATIVE |
| Units | pmol/mg tissue |
Chromatography:
| Chromatography ID: | CH000435 |
| Methods ID: | DQM0166 |
| Instrument Name: | QTOF-002-HILIC-35min-1mm-No Insert.m.zip |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000542 |
| Analysis ID: | AN000609 |
| Instrument Name: | Agilent 6410A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
