Summary of Study ST000376

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000296. The data can be accessed directly via it's Project DOI: 10.21228/M8SC7G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000376
Study TitleKidney choline acetyltransferase (ChAT)-3
Study TypeRegular
Study SummaryWe use ChAT(BAC)-EGFP mice, which express enhanced green fluorescent protein (EGFP) under the control of transcriptional regulatory elements for choline acetyltransferase (ChAT), the sole enzyme that catalyzes the biosynthesis of acetylcholine (ACh). These mice were treated with inducers of ChAT. Kidney were rapidly removed, frozen on liquid nitrogen and stored at -80C
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-04-16
Num Groups7
Total Subjects25
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SC7G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000296
Project DOI:doi: 10.21228/M8SC7G
Project Title:Role of kidney acetylcholine in protection from kidney injury
Project Summary:ChAT was induced in a model of kidney inflammation to determine its role in kidney protection
Institute:University of Colorado Denver
Department:Medicine
Laboratory:Garcia Lab
Last Name:Garcia
First Name:Gabriela
Address:Denver, CO
Email:Gabriela.garcia@ucdenver.edu
Phone:303-724-4897

Subject:

Subject ID:SU000397
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treated Gender Strain
SA017018S00018102Control Female ChAT
SA017019S00018101Control Female ChAT
SA017020S00018104Control Female WT
SA017021S00018103Control Female WT
SA017022S00018108Control Male ChAT
SA017023S00018109Control Male WT
SA017024S00018085Treated Female ChAT
SA017025S00018106Treated Female ChAT
SA017026S00018107Treated Female ChAT
SA017027S00018088Treated Female ChAT
SA017028S00018087Treated Female ChAT
SA017029S00018091Treated Female ChAT
SA017030S00018089Treated Female ChAT
SA017031S00018090Treated Female ChAT
SA017032S00018086Treated Female ChAT
SA017033S00018105Treated Male ChAT
SA017034S00018096Treated Male ChAT
SA017035S00018093Treated Male ChAT
SA017036S00018092Treated Male ChAT
SA017037S00018095Treated Male ChAT
SA017038S00018098Treated Male ChAT
SA017039S00018099Treated Male ChAT
SA017040S00018100Treated Male ChAT
SA017041S00018097Vehicle Male ChAT
SA017042S00018094Vehicle Male ChAT
Showing results 1 to 25 of 25

Collection:

Collection ID:CO000391
Collection Summary:none
Sample Type:Kidney

Treatment:

Treatment ID:TR000411
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000404
Sampleprep Summary:none
Sampleprep Protocol Filename:2014-10-07-Neurotransmitter.docx

Combined analysis:

Analysis ID AN000610
Analysis type MS
Chromatography type Normal phase
Chromatography system Unspecified
Column Cogent Diamond Hydride
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode POSITIVE
Units pmol/mg sample

Chromatography:

Chromatography ID:CH000436
Methods ID:DQM0150
Methods Filename:QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip
Instrument Name:Unspecified
Column Name:Cogent Diamond Hydride
Chromatography Type:Normal phase

MS:

MS ID:MS000543
Analysis ID:AN000610
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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