Summary of Study ST000378

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000297. The data can be accessed directly via it's Project DOI: 10.21228/M8NP5K This work is supported by NIH grant, U2C- DK119886.

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Study IDST000378
Study Title2 hydroxiglutarate with with IDH1-R132H inhibitor in neurospheres
Study TypeRegular
Study SummaryTumor neurospheres carrying genetic lession NPI (NRAS, shP53, IDH1-R132H) colony 1 and 2 (C1 and C2); NPAI (NRAS, shP53, shATRX, IDH1-R132H) colony 1 and 2 (C1 and C2); and NPA (NRAS, shP53, shATRX) (C1 and C2). Was treated with IDH1-R132H inhibitor. By 2HG assay we want to check the effect of the inhibitor in our cells.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-20
Num Groups3
Total Subjects12
Study Comments(shATRX) : ATRX knockdown sequence into a Sleeping Beauty (SB) transposase-responsive plasmid
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8NP5K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000297
Project DOI:doi: 10.21228/M8NP5K
Project Title:IDH1(R132H) mutations activity in neurospheres
Project Summary:assay 2HG procution in neurospheres expressing IDH1 mutated
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000399
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id shATRX plasmid IDH1-R132H mutation
SA017061S00018624no no
SA017062S00018487no yes
SA017063S00018488no yes
SA017064S00018625no yes
SA017065S00018489no yes
SA017066S00018490no yes
SA017067S00018620yes no
SA017068S00018622yes no
SA017069S00018498yes no
SA017070S00018496yes no
SA017071S00018497yes no
SA017072S00018495yes no
SA017073S00018492yes yes
SA017074S00018491yes yes
SA017075S00018493yes yes
SA017076S00018494yes yes
SA017077S00018621yes yes
SA017078S00018623yes yes
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000393
Collection Summary:none
Sample Type:Cell Line, Tumor

Treatment:

Treatment ID:TR000413
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000406
Sampleprep Summary:none
Sampleprep Protocol Filename:15DEC2015_2HG_cells_Castro.docx

Combined analysis:

Analysis ID AN000612
Analysis type MS
Chromatography type Reversed phase
Chromatography system None
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units ┬Ámol/g

Chromatography:

Chromatography ID:CH000438
Chromatography Summary:none
Methods ID:DQM0033
Methods Filename:2015-04-07-2HG.m.zip
Instrument Name:None
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000545
Analysis ID:AN000612
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:EX00452-2HG-QUANT-method.m.zip
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